Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

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Multiplex Genome Engineering Using CRISPR/Cas Systems

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RNA-Guided Human Genome Engineering via Cas9

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Genome-scale CRISPR-Cas9 knockout screening in human cells

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Search-and-replace genome editing without double-strand breaks or donor DNA

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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

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Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

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High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells

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In vivo genome editing using Staphylococcus aureus Cas9

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CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III

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Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

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The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

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Crystal structure of Cas9 in complex with guide RNA and target DNA

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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

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Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

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CRISPR–Cas9 Structures and Mechanisms

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Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

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Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

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Targeted genome modification of crop plants using a CRISPR-Cas system

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CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

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A CRISPR/Cas9 toolkit for multiplex genome editing in plants

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Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation

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Diversity, classification and evolution of CRISPR-Cas systems

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Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

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DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

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Global Warming, Climate Change, and Environmental Pollution: Recipe for a Multifactorial Stress Combination Disaster

Zandalinas, S. I., Fritschi, F., & Mittler, R. (2021) · Trends in Plant Science · doi:10.1016/j.tplants.2021.02.011 · Cited by 1,193

Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

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The Biology of CRISPR-Cas: Backward and Forward

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Climate change impacts on crop yield, crop water productivity and food security—A review

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Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

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ARGOS 8 variants generated by CRISPR ‐Cas9 improve maize grain yield under field drought stress conditions

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Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

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Redesigning photosynthesis to sustainably meet global food and bioenergy demand

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Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

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Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

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Applications of CRISPR–Cas in agriculture and plant biotechnology

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Broad-spectrum resistance to bacterial blight in rice using genome editing

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Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

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CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

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Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

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De novo domestication of wild tomato using genome editing

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Development of broad virus resistance in non‐transgenic cucumber using CRISPR/Cas9 technology

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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

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The CRISPR / C as9 system produces specific and homozygous targeted gene editing in rice in one generation

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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

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Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

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Engineering canker‐resistant plants through CRISPR /Cas9‐targeted editing of the susceptibility gene Cs LOB 1 promoter in citrus

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In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni

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Principle and application of plant mutagenesis in crop improvement: a review

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Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

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Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

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Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

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High-frequency, precise modification of the tomato genome

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Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

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Developing climate‐resilient crops: improving plant tolerance to stress combination

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Conformational control of DNA target cleavage by CRISPR–Cas9

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A Multifunctional AAV-CRISPR-Cas9 and Its Host Response

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Application of the CRISPR–Cas System for Efficient Genome Engineering in Plants

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Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice

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A Cas9–guide RNA complex preorganized for target DNA recognition

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Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

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Directed evolution of adenine base editors with increased activity and therapeutic application

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Simultaneous modification of three homoeologs of Ta EDR 1 by genome editing enhances powdery mildew resistance in wheat

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Rapid improvement of domestication traits in an orphan crop by genome editing

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Functionally diverse type V CRISPR-Cas systems

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CRISPR/Cas9-mediated viral interference in plants

Ali, Z., Abulfaraj, A. A., Idris, A. M., Ali, S., Tashkandi, M., & Mahfouz, M. M. (2015) · Genome biology · doi:10.1186/s13059-015-0799-6 · Cited by 519

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1443-z · Cited by 507

Genome editing of the disease susceptibility gene Cs LOB 1 in citrus confers resistance to citrus canker

Jia, H., Zhang, Y., Orbović, V., Xu, J. H., White, F. F., Jones, J. B., & Wang, N. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12677 · Cited by 500

A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds

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Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

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Am I ready for CRISPR? A user's guide to genetic screens

Doench, J. G. (2018) · Nature Reviews Genetics · doi:10.1038/nrg.2017.97 · Cited by 452

Novel alleles of rice eIF4G generated by CRISPR/Cas9‐targeted mutagenesis confer resistance to Rice tungro spherical virus

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Specific elimination of mutant mitochondrial genomes in patient-derived cells by mitoTALENs

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OsSAPK2 Confers Abscisic Acid Sensitivity and Tolerance to Drought Stress in Rice

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Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

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Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

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De Novo Domestication: An Alternative Route toward New Crops for the Future

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Precise plant genome editing using base editors and prime editors

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CRISPR/Cas: A powerful tool for gene function study and crop improvement

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CRISPR/cas9 genome editing in wheat

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Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes

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CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications

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Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice

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Gene Drives on the Horizon: Advancing Science, Navigating Uncertainty, and Aligning Research with Public Values

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Design of a bacterial speck resistant tomato by CRISPR /Cas9‐mediated editing of Sl JAZ 2

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Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

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Methodologies for Improving HDR Efficiency

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CRISPR plants now subject to tough GM laws in European Union

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