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Kinesin- and Myosin-driven Steps of Vesicle Recruitment for Ca2+-regulated Exocytosis

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Bi, Guo-Qiang, Morris, Robert L., Liao, Guochun, Alderton, Janet M., Scholey, Jonathan M., Steinhardt, Richard A.·1997·The Journal of Cell Biology
DOI 10.1083/jcb.138.5.999
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Abstract

Kinesin and myosin have been proposed to transport intracellular organelles and vesicles to the cell periphery in several cell systems. However, there has been little direct observation of the role of these motor proteins in the delivery of vesicles during regulated exocytosis in intact cells. Using a confocal microscope, we triggered local bursts of Ca2+-regulated exocytosis by wounding the cell membrane and visualized the resulting individual exocytotic events in real time. Different temporal phases of the exocytosis burst were distinguished by their sensitivities to reagents targeting different motor proteins. The function blocking antikinesin antibody SUK4 as well as the stalk-tail fragment of kinesin heavy chain specifically inhibited a slow phase, while butanedione monoxime, a myosin ATPase inhibitor, inhibited both the slow and fast phases. The blockage of Ca2+/calmodulin-dependent protein kinase II with autoinhibitory peptide also inhibited the slow and fast phases, consistent with disruption of a myosin-actin– dependent step of vesicle recruitment. Membrane resealing after wounding was also inhibited by these reagents. Our direct observations provide evidence that in intact living cells, kinesin and myosin motors may mediate two sequential transport steps that recruit vesicles to the release sites of Ca2+-regulated exocytosis, although the identity of the responsible myosin isoform is not yet known. They also indicate the existence of three semistable vesicular pools along this regulated membrane trafficking pathway. In addition, our results provide in vivo evidence for the cargo-binding function of the kinesin heavy chain tail domain.

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Metadata

Authors
Bi, G.-Q., Morris, R. L., Liao, G., Alderton, J. M., Scholey, J. M., & Steinhardt, R. A.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1997-09-08
Volume
138
Issue
5
Pages
999-1008
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.138.5.999
OpenAlex
W2061936950
Cited by
208
References
5
FWCI
8.70
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Kinesin- and Myosin-driven Steps of Vesicle Recruitment for Ca2+-regulated Exocytosis. https://app.cochat.ai/papers/10.1083/jcb.138.5.999
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Bi, G.-Q., Morris, R. L., Liao, G., Alderton, J. M., Scholey, J. M., & Steinhardt, R. A. (1997). Kinesin- and Myosin-driven Steps of Vesicle Recruitment for Ca2+-regulated Exocytosis. The Journal of Cell Biology. https://doi.org/10.1083/jcb.138.5.999

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