Circulating Skeletal Stem Cells
Open accessAbstract
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, “mesenchymal stem cells”). The osteogenic potential of the blood-borne cells was proven by an in vivo transplantation assay in which either polyclonal or single colony–derived strains were transplanted into the subcutis of immunocompromised mice, and the donor origin of the fully differentiated bone cells was proven using species-specific probes. This is the first definitive proof of the existence of circulating skeletal stem cells in mammals.
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Cells with true osteogenic potential can circulate.
“Cells with true osteogenic potential can also circulate.278”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [278]
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- Authors
- Kuznetsov, S. A., Mankani, M. H., Gronthos, S., Satomura, K., Bianco, P., & Robey, P. G.
- Affiliations
- National Institutes of Health (US)
- Tokushima University (JP)
- Sapienza University of Rome (IT)
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-05-28
- Volume
- 153
- Issue
- 5
- Pages
- 1133-1140
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2174322
- OpenAlex
- W2147155975
- Cited by
- 685
- References
- 21
- Citations by year
- 2025: 3 · 2024: 7 · 2023: 5 · 2022: 10 · 2021: 11 · 2020: 19 · 2019: 12 · 2018: 20 · 2017: 15 · 2016: 18 · 2015: 30 · 2014: 25 · 2013: 39 · 2012: 41
- FWCI
- 14.84
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- osteogenic differentiation, adipogenesis, fibroblast-like cells, clonogenic cells, bone regeneration, species-specific probes, mammalian blood
- MeSH terms
- Animals, Bone and Bones, Bone Development, Cell Adhesion, Cell Differentiation, Cells, Cultured, Clone Cells, Fibroblasts, Guinea Pigs, Hematopoietic Stem Cells, Humans, Immunohistochemistry, Mesoderm, Rabbits, Repetitive Sequences, Nucleic Acid, Skin, Species Specificity, Immunophenotyping, Stromal Cells, Adipocytes, Hematopoietic Stem Cell Transplantation, Cell Lineage, Cell Size, Mice
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