The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Mechanism of filopodia initiation by reorganization of a dendritic network
Open accessAbstract
Afilopodium protrudes by elongation of bundled actin filaments in its core. However, the mechanism of filopodia initiation remains unknown. Using live-cell imaging with GFP-tagged proteins and correlative electron microscopy, we performed a kinetic-structural analysis of filopodial initiation in B16F1 melanoma cells. Filopodial bundles arose not by a specific nucleation event, but by reorganization of the lamellipodial dendritic network analogous to fusion of established filopodia but occurring at the level of individual filaments. Subsets of independently nucleated lamellipodial filaments elongated and gradually associated with each other at their barbed ends, leading to formation of cone-shaped structures that we term Λ-precursors. An early marker of initiation was the gradual coalescence of GFP-vasodilator-stimulated phosphoprotein (GFP-VASP) fluorescence at the leading edge into discrete foci. The GFP-VASP foci were associated with Λ-precursors, whereas Arp2/3 was not. Subsequent recruitment of fascin to the clustered barbed ends of Λ-precursors initiated filament bundling and completed formation of the nascent filopodium. We propose a convergent elongation model of filopodia initiation, stipulating that filaments within the lamellipodial dendritic network acquire privileged status by binding a set of molecules (including VASP) to their barbed ends, which protect them from capping and mediate association of barbed ends with each other.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Control of actin dynamics in cell motility
Carlier, M.-F., & Pantaloni, D. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1997.1062 · Cited by 351
Cited by
RHO GTPASES: Biochemistry and Biology
Jaffe, A. B., & Hall, A. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.020604.150721 · Cited by 3,009
The actin cytoskeleton and actin-based motility
Svitkina, T. (2018) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a018267 · Cited by 761
Self-citationLamellipodial Versus Filopodial Mode of the Actin Nanomachinery
Mejillano, M. R., Kojima, S.-I., Applewhite, D. A., Gertler, F. B., Svitkina, T. M., & Borisy, G. G. (2004) · Cell · doi:10.1016/j.cell.2004.07.019 · Cited by 420
Self-citationNiessen, C. M., Leckband, D., & Yap, A. S. (2011) · Physiological Reviews · doi:10.1152/physrev.00004.2010 · Cited by 427
Korobova, F., & Svitkina, T. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-07-0596 · Cited by 399
Self-citationThe Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration
Suraneni, P., Rubinstein, B., Unruh, J. R., Durnin, M., Hanein, D., & Li, R. (2012) · Journal of Cell Biology · doi:10.1083/jcb.201112113 · Cited by 337
Organization of Actin Networks in Intact Filopodia
Medalia, O., Beck, M., Ecke, M., Weber, I., Neujahr, R., Baumeister, W., & Gerisch, G. (2007) · Current Biology · doi:10.1016/j.cub.2006.11.022 · Cited by 170
Molecular basis for SH3 domain regulation of F-BAR–mediated membrane deformation
Rao, Y., Ma, Q., Vahedi-Faridi, A., Sundborger, A., Pechstein, A., Puchkov, D., Luo, L., Shupliakov, O., Saenger, W., & Haucke, V. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1003478107 · Cited by 149
Harris, A. R., Jreij, P., & Fletcher, D. A. (2018) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-070816-033547 · Cited by 140
Synaptic Actin Dysregulation, a Convergent Mechanism of Mental Disorders?
Yan, Z., Kim, E., Datta, D., Lewis, D. A., & Soderling, S. H. (2016) · The Journal of Neuroscience · doi:10.1523/jneurosci.2360-16.2016 · Cited by 120
Cytoskeletal and signaling mechanisms of neurite formation
Sainath, R., & Gallo, G. (2015) · Cell and Tissue Research · doi:10.1007/s00441-014-1955-0 · Cited by 116
Unravelling the actin cytoskeleton: a new competitive edge?
Davidson, A. J., & Wood, W. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.04.001 · Cited by 110
Coro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis
Ho, C. T., Evans, E. B., Lukasik, K., O’Shaughnessy, E. C., Shah, A., Hsu, C.-H., Temple, B., Bear, J. E., & Gupton, S. L. (2025) · Journal of Cell Biology · doi:10.1083/jcb.202503068 · Cited by 1
Metadata
- Authors
- Svitkina, T. M., Bulanova, E. A., Chaga, O. Y., Vignjevic, D. M., Kojima, S.-I., Vasiliev, J. M., & Borisy, G. G.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-02-03
- Volume
- 160
- Issue
- 3
- Pages
- 409-421
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2146894615
- Cited by
- 770
- References
- 1
- FWCI
- 17.64
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
