The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed
Open accessAbstract
How focal adhesions (FAs) convert retrograde filamentous actin (F-actin) flow into traction stress on the extracellular matrix to drive cell migration is unknown. Using combined traction force and fluorescent speckle microscopy, we observed a robust biphasic relationship between F-actin speed and traction force. F-actin speed is inversely related to traction stress near the cell edge where FAs are formed and F-actin motion is rapid. In contrast, larger FAs where the F-actin speed is low are marked by a direct relationship between F-actin speed and traction stress. We found that the biphasic switch is determined by a threshold F-actin speed of 8–10 nm/s, independent of changes in FA protein density, age, stress magnitude, assembly/disassembly status, or subcellular position induced by pleiotropic perturbations to Rho family guanosine triphosphatase signaling and myosin II activity. Thus, F-actin speed is a fundamental regulator of traction force at FAs during cell migration.
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Metadata
- Authors
- Gardel, M. L., Sabass, B., Ji, L., Danuser, G., Schwarz, U. S., & Waterman, C. M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2008-12-15
- Volume
- 183
- Issue
- 6
- Pages
- 999-1005
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2153626442
- Cited by
- 490
- References
- 25
- FWCI
- 8.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Gardel, M. L., Sabass, B., Ji, L., Danuser, G., Schwarz, U. S., & Waterman, C. M. (2008). Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200810060
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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