The DOI matches its Crossref record; 79 of the 79 references it cites were found in a registry or an index.
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
Open accessAbstract
The catalytic activity of the MPS1 kinase is crucial for the spindle assembly checkpoint and for chromosome biorientation on the mitotic spindle. We report that the small molecule reversine is a potent mitotic inhibitor of MPS1. Reversine inhibits the spindle assembly checkpoint in a dose-dependent manner. Its addition to mitotic HeLa cells causes the ejection of Mad1 and the ROD–ZWILCH–ZW10 complex, both of which are important for the spindle checkpoint, from unattached kinetochores. By using reversine, we also demonstrate that MPS1 is required for the correction of improper chromosome–microtubule attachments. We provide evidence that MPS1 acts downstream from the AURORA B kinase, another crucial component of the error correction pathway. Our experiments describe a very useful tool to interfere with MPS1 activity in human cells. They also shed light on the relationship between the error correction pathway and the spindle checkpoint and suggest that these processes are coregulated and are likely to share at least a subset of their catalytic machinery.
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Cites
Checkpoints: Controls That Ensure the Order of Cell Cycle Events
Hartwell, L. H., & Weinert, T. A. (1989) · Science · doi:10.1126/science.2683079 · Cited by 3,021
The spindle-assembly checkpoint in space and time
Musacchio, A., & Salmon, E. D. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2163 · Cited by 2,227
Self-citationDissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
Straight, A. F., Cheung, A., Limouze, J., Chen, I., Westwood, N. J., Sellers, J. R., & Mitchison, T. J. (2003) · Science · doi:10.1126/science.1081412 · Cited by 1,412
Ditchfield, C., Johnson, V. L., Tighe, A., Ellston, R., Haworth, C., Johnson, T., Mortlock, A., Keen, N., & Taylor, S. S. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200208091 · Cited by 1,194
Self-citationHauf, S., Cole, R. W., LaTerra, S., Zimmer, C., Schnapp, G., Walter, R., Heckel, A., van Meel, J., Rieder, C. L., & Peters, J.-M. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200208092 · Cited by 1,155
Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins
Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K., & Koike, T. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t500024-mcp200 · Cited by 1,101
Cleveland, D. W., Mao, Y., & Sullivan, K. F. (2003) · Cell · doi:10.1016/s0092-8674(03)00115-6 · Cited by 1,003
The Conserved KMN Network Constitutes the Core Microtubule-Binding Site of the Kinetochore
Cheeseman, I. M., Chappie, J. S., Wilson-Kubalek, E. M., & Desai, A. (2006) · Cell · doi:10.1016/j.cell.2006.09.039 · Cited by 994
Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles
Glover, D. M., Leibowitz, M. H., McLean, D. A., & Parry, H. (1995) · Cell · doi:10.1016/0092-8674(95)90374-7 · Cited by 836
Chromosomal passengers: conducting cell division
Ruchaud, S., Carmena, M., & Earnshaw, W. C. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2257 · Cited by 811
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Hein, M. Y., Hubner, N. C., Poser, I., Cox, J., Nagaraj, N., Toyoda, Y., Gak, I. A., Weisswange, I., Mansfeld, J., Buchholz, F., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.053 · Cited by 1,270
G&T-seq: parallel sequencing of single-cell genomes and transcriptomes
Macaulay, I. C., Haerty, W., Kumar, P., Li, Y. I., Hu, T. X., Teng, M. J., Goolam, M., Saurat, N., Coupland, P., Shirley, L. M., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3370 · Cited by 750
Mitotic catastrophe: a mechanism for avoiding genomic instability
Vitale, I., Galluzzi, L., Castedo, M., & Kroemer, G. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3115 · Cited by 834
Telomeres in cancer: tumour suppression and genome instability
Maciejowski, J., & de Lange, T. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.171 · Cited by 745
The molecular biology of spindle assembly checkpoint signaling dynamics.
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Metadata
- Authors
- Santaguida, S., Tighe, A., D'Alise, A. M., Taylor, S. S., & Musacchio, A.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-07-12
- Volume
- 190
- Issue
- 1
- Pages
- 73-87
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2171175279
- Cited by
- 531
- References
- 79
- FWCI
- 12.79
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Santaguida, S., Tighe, A., D'Alise, A. M., Taylor, S. S., & Musacchio, A. (2010). Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. Journal of Cell Biology. https://doi.org/10.1083/jcb.201001036
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