Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation
Open accessAbstract
During differentiation, many cells reorganize their microtubule cytoskeleton into noncentrosomal arrays. Although these microtubules are likely organized to meet the physiological roles of their tissues, their functions in most cell types remain unexplored. In the epidermis, differentiation induces the reorganization of microtubules to cell–cell junctions in a desmosome-dependent manner. Here, we recapitulate the reorganization of microtubules in cultured epidermal cells. Using this reorganization assay, we show that cortical microtubules recruit myosin II to the cell cortex in order to engage adherens junctions, resulting in an increase in mechanical integrity of the cell sheets. Cortical microtubules and engaged adherens junctions, in turn, increase tight junction function. In vivo, disruption of microtubules or loss of myosin IIA and B resulted in loss of tight junction–mediated barrier activity. We propose that noncentrosomal microtubules act through myosin II recruitment to potentiate cell adhesion in the differentiating epidermis, thus forming a robust mechanical and chemical barrier against the external environment.
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Myosin-II-mediated junctional tension in the epidermis is required for TJ barrier function.
“The observation that myosin-II-mediated junctional tension in the epidermis was shown to be required for TJ barrier function44”
From E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning (2017) · cites this paper as [44]
The Results section, “Loss of myosin IIA/B in the epidermis leads to tight junction defects” (page unavailable), reports a profound tight-junction functional defect in myosin IIA/B–null epidermis. The Discussion links myosin-dependent junctional strengthening to barrier function, but says the data *suggest* that adherens-junction tension controls tight-junction activity; it does not establish that junctional tension itself is required. (LLM, conf=0.82)
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Cites
Claudin-based tight junctions are crucial for the mammalian epidermal barrier
Furuse, M., Hata, M., Furuse, K., Yoshida, Y., Haratake, A., Sugitani, Y., Noda, T., Kubo, A., & Tsukita, S. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200110122 · Cited by 1,512 · see citing works
Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
Straight, A. F., Cheung, A., Limouze, J., Chen, I., Westwood, N. J., Sellers, J. R., & Mitchison, T. J. (2003) · Science · doi:10.1126/science.1081412 · Cited by 1,412 · see citing works
The MAP1 family of microtubule-associated proteins.
Dehmelt (2004) · Genome biology · doi:10.1186/gb-2004-6-1-204 · Cited by 972 · see citing works
α-Catenin as a tension transducer that induces adherens junction development
Yonemura, S., Wada, Y., Watanabe, T., Nagafuchi, A., & Shibata, M. (2010) · Nature Cell Biology · doi:10.1038/ncb2055 · Cited by 972 · see citing works
Gumbiner, B., Stevenson, B., & Grimaldi, A. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.4.1575 · Cited by 799 · see citing works
Mechanisms of Taxol resistance related to microtubules
Orr, G. A., Verdier-Pinard, P., McDaid, H., & Horwitz, S. B. (2003) · Oncogene · doi:10.1038/sj.onc.1206934 · Cited by 727 · see citing works
Mechanical tugging force regulates the size of cell–cell junctions
Liu, Z., Tan, J. L., Cohen, D. M., Yang, M. T., Sniadecki, N. J., Ruiz, S. A., Nelson, C. M., & Chen, C. S. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914547107 · Cited by 704 · see citing works
le Duc, Q., Shi, Q., Blonk, I., Sonnenberg, A., Wang, N., Leckband, D., & de Rooij, J. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201001149 · Cited by 652 · see citing works
E‐cadherin is essential for in vivo epidermal barrier function by regulating tight junctions
Tunggal, J. A., Helfrich, I., Schmitz, A., Schwarz, H., Günzel, D., Fromm, M., Kemler, R., Krieg, T., & Niessen, C. M. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600605 · Cited by 491 · cited 2× · see citing works
“Because adherens junction components are known to be essential for proper tight junction assembly (Gumbiner et al., 1988; Tunggal et al., 2005), we analyzed the effect of taxol treatment on tight junction function in cultured cells, as measured by transepithelial resistance (TER).”
Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure
Shen, L., Black, E. D., Witkowski, E. D., Lencer, W. I., Guerriero, V., Schneeberger, E. E., & Turner, J. R. (2006) · Journal of Cell Science · doi:10.1242/jcs.02915 · Cited by 467 · see citing works
Patterned acquisition of skin barrier function during development
Hardman, M. J., Sisi, P., Banbury, D. N., & Byrne, C. (1998) · Development · doi:10.1242/dev.125.8.1541 · Cited by 385 · see citing works
Shen, L., Weber, C. R., & Turner, J. R. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200711165 · Cited by 361 · see citing works
Substoichiometric Binding of Taxol Suppresses Microtubule Dynamics
Derry, W. B., Wilson, L. R., & Jordan, M. A. (1995) · Biochemistry · doi:10.1021/bi00007a014 · Cited by 335 · see citing works
Smutny, M., Cox, H. L., Leerberg, J. M., Kovács, É., Conti, M. A., Ferguson, C., Hamilton, N., Parton, R. G., Adelstein, R. S., & Yap, A. S. (2010) · Nature Cell Biology · doi:10.1038/ncb2072 · Cited by 332 · see citing works
Delgehyr, N., Sillibourne, J., & Bornens, M. (2005) · Journal of Cell Science · doi:10.1242/jcs.02302 · Cited by 293 · see citing works
Microtubule Organization and Function in Epithelial Cells
Müsch, A. (2003) · Traffic · doi:10.1111/j.1600-0854.2003.00149.x · Cited by 268 · see citing works
p120-Catenin Mediates Inflammatory Responses in the Skin
Pérez‐Moreno, M., Davis, M. A., Wong, E., Pasolli, H. A., Reynolds, A. B., & Fuchs, E. V. (2006) · Cell · doi:10.1016/j.cell.2005.11.043 · Cited by 264 · see citing works
Molecular Bases of Cell-Cell Junctions Stability and DynamicsPreprint
Cavey, M., & Lecuit, T. (2009) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a002998 · Cited by 257 · see citing works
Yu, D., Marchiando, A. M., Weber, C. R., Raleigh, D. R., Wang, Y., Shen, L., & Turner, J. R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908869107 · Cited by 256 · see citing works
Walsh, S. V., Hopkins, A. M., Chen, J. Z., Narumiya, S., Parkos, C. A., & Nusrat, A. (2001) · Gastroenterology · doi:10.1053/gast.2001.27060 · Cited by 211 · see citing works
Hopkins, A. M., Walsh, S. V., Verkade, P., Boquet, P., & Nusrat, A. (2003) · Journal of Cell Science · doi:10.1242/jcs.00300 · Cited by 202 · see citing works
Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
Lechler, T., & Fuchs, E. V. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200609109 · Cited by 186 · see citing works
Self-citationDynamic microtubules regulate the local concentration of E-cadherin at cell-cell contacts
Stehbens, S. J., Paterson, A. D., Crampton, M. S., Shewan, A. M., Ferguson, C., Akhmanova, A. S., Parton, R. G., & Yap, A. S. (2006) · Journal of Cell Science · doi:10.1242/jcs.02903 · Cited by 185 · see citing works
A Unique Role for Nonmuscle Myosin Heavy Chain IIA in Regulation of Epithelial Apical Junctions
Ivanov, A. I., Bachar, M., Babbin, B. A., Adelstein, R. S., Nusrat, A., & Parkos, C. A. (2007) · PLoS ONE · doi:10.1371/journal.pone.0000658 · Cited by 178 · see citing works
Mechanosensitive EPLIN-dependent remodeling of adherens junctions regulates epithelial reshaping
Taguchi, K., Ishiuchi, T., & Takeichi, M. (2011) · The Journal of Cell Biology · doi:10.1083/jcb.201104124 · Cited by 159 · see citing works
Microtubule cortical array organization and plant cell morphogenesis
Paradez, A., Wright, A. J., & Ehrhardt, D. W. (2006) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2006.09.005 · Cited by 156 · see citing works
New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity
Tinkle, C. L., Pasolli, H. A., Stokes, N., & Fuchs, E. V. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0807374105 · Cited by 134 · see citing works
Ma, X., Jana, S. S., Conti, M. A., Kawamoto, S., Claycomb, W. C., & Adelstein, R. S. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-04-0293 · Cited by 117 · see citing works
Role of aPKC Isoforms and Their Binding Partners Par3 and Par6 in Epidermal Barrier Formation
Helfrich, I., Schmitz, A., Zigrino, P., Michels, C., Haase, I., Bivic, A. L., Leitges, M., & Niessen, C. M. (2006) · Journal of Investigative Dermatology · doi:10.1038/sj.jid.5700621 · Cited by 112 · see citing works
Guo, J., Yang, Z., Song, W., Chen, Q., Wang, F., Zhang, Q., & Zhu, X. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-04-0360 · Cited by 91 · see citing works
Lamellar Bodies of Human Epidermis
Raymond, A.-A., de Peredo, A. G., Stella, A., Ishida‐Yamamoto, A., Bouyssié, D., Serre, G., Monsarrat, B., & Simon, M. (2008) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700334-mcp200 · Cited by 87 · see citing works
Pizon, V., Gerbal, F., Cifuentes-Díaz, C., & Karsenti, E. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600842 · Cited by 82 · see citing works
Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis
Sumigray, K., Chen, H., & Lechler, T. (2011) · The Journal of Cell Biology · doi:10.1083/jcb.201104009 · Cited by 81 · see citing works
Self-citationMicrotubules Tethered at Epithelial Cell Junctions by Dynein Facilitate Efficient Junction Assembly
Ligon, L. A., & Holzbaur, E. L. F. (2007) · Traffic · doi:10.1111/j.1600-0854.2007.00574.x · Cited by 80 · see citing works
Patel, S., Xi, Z. F., Seo, E. Y., McGaughey, D. M., & Segre, J. A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608658103 · Cited by 69 · see citing works
Kee, S., & Steinert, P. M. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.7.1983 · Cited by 31 · see citing works
Mattiuzzo, N. R., Toulza, È., Jonca, N., Serre, G., & Guerrin, M. (2011) · Experimental Dermatology · doi:10.1111/j.1600-0625.2010.01188.x · Cited by 18 · see citing works
Rapid Microwave Fixation of Cell Monolayers Preserves Microtubule-associated Cell Structures
Reipert, S., Kotisch, H., Wysoudil, B., & Wiche, G. (2008) · Journal of Histochemistry & Cytochemistry · doi:10.1369/jhc.7a7370.2008 · Cited by 18 · see citing works
Jonca, N., Masson, D., Viard, I., & French, L. E. (1999) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.1999.00500.x · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Actin–microtubule crosstalk in cell biology
Dogterom, M., & Koenderink, G. H. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0067-1 · Cited by 563 · see citing works
Rübsam, M., Mertz, A. F., Kubo, A., Marg, S., Jüngst, C., Goranci-Buzhala, G., Schauss, A. C., Horsley, V., Dufresne, E. R., Moser, M., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-01170-7 · Cited by 210 · see citing works
Microtubule organization, dynamics and functions in differentiated cells
Muroyama, A., & Lechler, T. (2017) · Development · doi:10.1242/dev.153171 · Cited by 204 · see citing works
Self-citationRemodeling the zonula adherens in response to tension and the role of afadin in this response
Choi, W., Acharya, B. R., Peyret, G., Fardin, M.-A., Mège, R., Ladoux, B., Yap, A. S., Fanning, A. S., & Peifer, M. A. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201506115 · Cited by 191 · see citing works
Desmosome assembly and dynamics
Nekrasova, O. E., & Green, K. J. (2013) · Trends in Cell Biology · doi:10.1016/j.tcb.2013.06.004 · Cited by 177 · see citing works
Integrins and epithelial cell polarity
Lee, J. L., & Streuli, C. H. (2014) · Journal of Cell Science · doi:10.1242/jcs.146142 · Cited by 157 · see citing works
Mechanical Forces in the Skin: Roles in Tissue Architecture, Stability, and Function
Biggs, L. C., Kim, C. S. K., Miroshnikova, Y. A., & Wickström, S. A. (2019) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2019.06.137 · Cited by 151 · see citing works
Rübsam, M., Broussard, J. A., Wickström, S. A., Nekrasova, O. E., Green, K. J., & Niessen, C. M. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a029207 · Cited by 148 · see citing works
Villeneuve, C., Hashmi, A., Ylivinkka, I., Lawson-Keister, E., Miroshnikova, Y. A., Gonzalez, C. P., Myllymäki, S.-M., Bertillot, F., Yadav, B., Zhang, T., et al. (2024) · Nature Cell Biology · doi:10.1038/s41556-023-01332-4 · Cited by 121 · see citing works
Wang, S., Wu, D., Quintin, S., Green, R. A., Cheerambathur, D. K., Ochoa, S. D., Desai, A. B., & Oegema, K. (2015) · eLife · doi:10.7554/elife.08649 · Cited by 115 · see citing works
Cell Adhesion in Epidermal Development and Barrier Formation
Sumigray, K., & Lechler, T. (2015) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2014.11.027 · Cited by 110 · see citing works
Self-citationRevenu, C., Streichan, S. J., Doná, E., Lecaudey, V., Hufnagel, L., & Gilmour, D. T. (2014) · Development · doi:10.1242/dev.101675 · Cited by 109 · see citing works
Desmosomes: Essential contributors to an integrated intercellular junction network
Green, K. J., Jaiganesh, A., & Broussard, J. A. (2019) · F1000Research · doi:10.12688/f1000research.20942.1 · Cited by 94 · see citing works
Divergent regulation of functionally distinct γ-tubulin complexes during differentiation
Muroyama, A., Seldin, L., & Lechler, T. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201601099 · Cited by 82 · see citing works
Self-citationDifferentiated Daughter Cells Regulate Stem Cell Proliferation and Fate through Intra-tissue Tension
Ning, W., Muroyama, A., Li, H., & Lechler, T. (2020) · Cell stem cell · doi:10.1016/j.stem.2020.11.002 · Cited by 79 · see citing works
Self-citationZhou, K., Muroyama, A., Underwood, J., Leylek, R. A., Ray, S., Soderling, S. H., & Lechler, T. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1308419110 · Cited by 78 · see citing works
Self-citationDynamic microtubules produce an asymmetric E-cadherin–Bazooka complex to maintain segment boundaries
Bulgakova, N. A., Grigoriev, I., Yap, A. S., Akhmanova, A. S., & Brown, N. H. (2013) · The Journal of Cell Biology · doi:10.1083/jcb.201211159 · Cited by 73 · see citing works
Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions
Stewart, R. M., Zubek, A., Rosowski, K. A., Schreiner, S. M., Horsley, V., & King, M. C. (2015) · The Journal of Cell Biology · doi:10.1083/jcb.201502024 · Cited by 69 · see citing works
β-Catenin protects the epidermis from mechanical stresses
Ray, S., Foote, H. P., & Lechler, T. (2013) · The Journal of Cell Biology · doi:10.1083/jcb.201212140 · Cited by 51 · see citing works
Self-citationCLASP2 interacts with p120-catenin and governs microtubule dynamics at adherens junctions
Shahbazi, M. N., Megı́as, D., Epifano, C., Akhmanova, A. S., Gundersen, G. G., Fuchs, E. V., & Pérez‐Moreno, M. (2013) · The Journal of Cell Biology · doi:10.1083/jcb.201306019 · Cited by 42 · see citing works
Muroyama, A., & Lechler, T. (2017) · eLife · doi:10.7554/elife.29834 · Cited by 42 · see citing works
Self-citationQuintin, S., Wang, S., Pontabry, J., Bender, A., Robin, F., Hyenne, V., Landmann, F., Gally, C., Oegema, K., & Labouesse, M. (2015) · Development · doi:10.1242/dev.126615 · Cited by 39 · see citing works
Glotfelty, L., Zahs, A., Hodges, K., Shan, K., Alto, N. M., & Hecht, G. A. (2014) · Cellular Microbiology · doi:10.1111/cmi.12323 · Cited by 39 · see citing works
FACEts of mechanical regulation in the morphogenesis of craniofacial structures
Du, W. T., Bhojwani, A., & Hu, J. K. (2021) · International Journal of Oral Science · doi:10.1038/s41368-020-00110-4 · Cited by 33 · see citing works
Hereditary barrier-related diseases involving the tight junction: lessons from skin and intestine
Brandner, J. M., & Schulzke, J. D. (2015) · Cell and Tissue Research · doi:10.1007/s00441-014-2096-1 · Cited by 33 · see citing works
Epidermal Polarity Genes in Health and Disease
Tellkamp, F., Vorhagen, S., & Niessen, C. M. (2014) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a015255 · Cited by 33 · see citing works
Perl, A. L., Pokorny, J. L., & Green, K. J. (2024) · Journal of Cell Science · doi:10.1242/jcs.261899 · Cited by 32 · see citing works
Microtubule architecture connects AMOT stability to YAP/TAZ mechanotransduction and Hippo signalling
Vanni, G., Citron, A., Suli, A., Contessotto, P., Caire, R., Gandin, A., Mantovan, G., Zanconato, F., Brusatin, G., Palma, M. D., et al. (2025) · Nature Cell Biology · doi:10.1038/s41556-025-01773-z · Cited by 32 · see citing works
Cell-Cell Adhesions and Cell Contractility Are Upregulated upon Desmosome Disruption
Sumigray, K., Zhou, K., & Lechler, T. (2014) · PLoS ONE · doi:10.1371/journal.pone.0101824 · Cited by 32 · see citing works
Self-citationThymosin β4 is essential for adherens junction stability and epidermal planar cell polarity
Padmanabhan, K., Grobe, H., Cohen, J., Soffer, A., Mahly, A., Adir, O., Zaidel‐Bar, R., & Luxenburg, C. (2020) · Development · doi:10.1242/dev.193425 · Cited by 32 · see citing works
Foote, H. P., Sumigray, K., & Lechler, T. (2013) · PLoS ONE · doi:10.1371/journal.pone.0071491 · Cited by 32 · see citing works
Self-citationProteomic analysis of desmosomes reveals novel components required for epidermal integrity
Badu-Nkansah, K. A., & Lechler, T. (2020) · Molecular Biology of the Cell · doi:10.1091/mbc.e19-09-0542 · Cited by 27 · see citing works
Self-citationZhang, X., Jin, J. Y., WU, J. M., Qin, X., Streilein, R. D., Hall, R. P., & Zhang, J. Y. (2014) · Journal of Investigative Dermatology · doi:10.1038/jid.2014.519 · Cited by 27 · see citing works
An ELMO2-RhoG-ILK network modulates microtubule dynamics
Jackson, B. C., Ivanova, I. A., & Dagnino, L. (2015) · Molecular Biology of the Cell · doi:10.1091/mbc.e14-10-1444 · Cited by 26 · see citing works
Sayedyahossein, S., Rudkouskaya, A., Leclerc, V., & Dagnino, L. (2015) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2015.10.056 · Cited by 24 · see citing works
AJUBA: A regulator of epidermal homeostasis and cancer
Schleicher, K., & Schramek, D. (2020) · Experimental Dermatology · doi:10.1111/exd.14272 · Cited by 21 · see citing works
Cytoskeleton, intercellular junctions, planar cell polarity, and cell movement in amelogenesis
Nishikawa, S. (2017) · Journal of Oral Biosciences · doi:10.1016/j.job.2017.07.002 · Cited by 20 · see citing works
An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis
Laurin, M., Gomez, N. C., Levorse, J. M., Sendoel, A., Sribour, M., & Fuchs, E. V. (2019) · eLife · doi:10.7554/elife.50226 · Cited by 20 · see citing works
Crish, J. F., Conti, M. A., Sakai, T., Adelstein, R. S., & Egelhoff, T. T. (2013) · Developmental Biology · doi:10.1016/j.ydbio.2013.07.017 · Cited by 20 · see citing works
Simard‐Bisson, C., Bidoggia, J., Larouche, D., Guérin, S. L., Blouin, R., Hirai, S.-I., & Germain, L. (2016) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2016.07.035 · Cited by 19 · see citing works
Epidermal development requires ninein for spindle orientation and cortical microtubule organization
Lecland, N., Hsu, C., Chemin, C., Merdes, A., & Bierkamp, C. (2019) · Life Science Alliance · doi:10.26508/lsa.201900373 · Cited by 19 · see citing works
Hsu, C., Lecland, N., Pendaries, V., Viodé, C., Redoules, D., Paul, C. F., Merdes, A., Simon, M., & Bierkamp, C. (2018) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2018.04.008 · Cited by 17 · see citing works
Nguyen-Ngoc, K.-V., Silvestri, V. L., Georgess, D., Fairchild, A. N., & Ewald, A. J. (2017) · Journal of Cell Science · doi:10.1242/jcs.208546 · Cited by 15 · see citing works
The third dimension of the actin cortex
Jawahar, A., Vermeil, J., Heuvingh, J., du Roure, O., & Piel, M. (2024) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2024.102381 · Cited by 11 · see citing works
Youssef, G., Gerner, L., Naeem, A. S., Ralph, O. G., Ono, M., O’Neill, C. A., & O’Shaughnessy, R. F. L. (2013) · Developmental Biology · doi:10.1016/j.ydbio.2013.04.034 · Cited by 11 · see citing works
Kasai, Y., Takeda, N., Kobayashi, S., Takagi, R., & Yamato, M. (2017) · FEBS Open Bio · doi:10.1002/2211-5463.12213 · Cited by 11 · see citing works
Roles for microtubules in the proliferative and differentiated cells of stratified epithelia
Hlavaty, D., & Lechler, T. (2020) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2020.10.002 · Cited by 11 · see citing works
Self-citationNon-centrosomal Microtubule Organization in Differentiated Cells
Dyachuk, V. A., Bierkamp, C., & Merdes, A. (2016) · Springer eBooks · doi:10.1007/978-3-7091-1903-7_2 · Cited by 9 · see citing works
Xu, G., Yang, T., Wang, D., Li, J., Liu, X., Wu, X., & Shen, H. (2018) · PLoS ONE · doi:10.1371/journal.pone.0196252 · Cited by 9 · see citing works
Spectrin coordinates cell shape and signaling essential for epidermal differentiation
Soffer, A., Bhosale, A., Ghodrat, R., Peskoller, M., Matsui, T., Niessen, C. M., Luxenburg, C., & Rübsam, M. (2025) · The Journal of Cell Biology · doi:10.1083/jcb.202502071 · Cited by 6 · see citing works
Noncentrosomal microtubules in C. elegans epithelia
Quintin, S., Gally, C., & Labouesse, M. (2016) · genesis · doi:10.1002/dvg.22921 · Cited by 6 · see citing works
Arp2/3 complex function in the epidermis
Lechler, T. (2014) · Tissue Barriers · doi:10.4161/21688362.2014.944445 · Cited by 6 · see citing works
Self-citationPrado-Mantilla, A., Ning, W., & Lechler, T. (2024) · eLife · doi:10.7554/elife.100961 · Cited by 6 · see citing works
Self-citationClasp2 ensures mitotic fidelity and prevents differentiation of epidermal keratinocytes
Shahbazi, M. N., Peña-Jiménez, D., Antonucci, F., Drosten, M., & Pérez‐Moreno, M. (2017) · Journal of Cell Science · doi:10.1242/jcs.194787 · Cited by 4 · see citing works
Kobori, M., Abe, J., Saito, R., & Hirai, Y. (2024) · Experimental Cell Research · doi:10.1016/j.yexcr.2024.113927 · Cited by 4 · see citing works
Organization of cortical microtubules in differentiated cells
Zhou, P., Yang, G., & Xie, W. D. (2023) · Journal of Cellular Physiology · doi:10.1002/jcp.31011 · Cited by 4 · see citing works
A conformational change in α-catenin’s actin-binding domain governs adherens junction maturation
Windgasse, L., & Grashoff, C. (2025) · Communications Biology · doi:10.1038/s42003-025-08785-3 · Cited by 3 · see citing works
Microtubule organization: A complex solution
Conduit, P. T. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201606008 · Cited by 3 · see citing works
Desmosomes compartmentalize mRNA and translation in the skin
D’Alessandro, A., Badu-Nkansah, K. A., Link, S., Hlavaty, D., Bjerke, G., Nicchitta, C. V., Yi, R., & Lechler, T. (2026) · Developmental Cell · doi:10.1016/j.devcel.2026.02.013 · Cited by 2 · see citing works
Self-citationMechanisms of cortical microtubule organization in epidermal keratinocytes
Guo, K., & Merdes, A. (2025) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-025-05714-9 · Cited by 2 · see citing works
Matsunaga, H., Nozaki, S., Abe, J., & Hirai, Y. (2026) · Experimental Cell Research · doi:10.1016/j.yexcr.2026.114925 · Cited by 1 · see citing works
Only skin deep, thanks to microtubules
Leslie, M. (2012) · The Journal of Cell Biology · doi:10.1083/jcb.1993iti2 · Cited by 1 · see citing works
The role of the centrosome in mitotic spindle formation.
Tas, R. P. (2014) · Utrecht University Repository (Utrecht University)
Distinct Microtubule Subsets Organize Around The Newly Forming Lumen During Epithelial Polarization
Smith, W. (2015) · eCommons (Cornell University)
Unravelling gene regulation in the epidermis during differentiation
Rahim, A. B. A. (2018) · doi:10.32657/10356/75891
Muroyama, A., & Lechler, T. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/155994
Self-citationThe Myriad Ways Enteropathogenic Escherichia coli (EPEC) Alters Tight Junctions
Tapia, R., & Hecht, G. A. (2022) · Springer eBooks · doi:10.1007/978-3-030-97204-2_7
Molecular and Mechanical Signatures Contributing to Epidermal Differentiation and Barrier FormationPreprint
Prado-Mantilla, A., Ning, W., & Lechler, T. (2024) · eLife · doi:10.7554/elife.100961.1
Self-citationMolecular and Mechanical Signatures Contributing to Epidermal Differentiation and Barrier FormationPreprint
Prado-Mantilla, A., Ning, W., & Lechler, T. (2025) · eLife · doi:10.7554/elife.100961.2
Self-citationBouguenina, M. E. H. (2016) · doi:10.70675/75827ac7zf324z484czb3a7zdfe17ea901ef
Epidermal cells sculpt sensory nerve endings through actomyosin contractilityPreprint
Lee, M., Underwood, J., Xu, J., Ji, R., & Lechler, T. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.08.28.747813
Self-citation
- Authors
- Sumigray, K. D., Foote, H. P., & Lechler, T.
- Affiliations
- Duke Medical Center (US)
- Corresponding author
- Lechler, Terry
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2012-10-22
- Volume
- 199
- Issue
- 3
- Pages
- 513-525
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC3483132
- OpenAlex
- W2121533701
- Cited by
- 77
- References
- 39
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 8 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 6 · 2019: 5 · 2018: 4 · 2017: 9 · 2016: 7 · 2015: 8 · 2014: 8 · 2013: 8 · 2012: 1
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- March of Dimes Foundation
- Whitehead Foundation
- National Institutes of Health
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR055926)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- myosin II, myosin IIA, myosin IIB, epidermal differentiation, cell adhesion, skin barrier, adherens junctions, tight junctions, desmosomes, cortical microtubules, epidermal keratinocytes
- MeSH terms
- Epidermal Cells, Animals, Cell Adhesion, Cell Differentiation, Cells, Cultured, Desmosomes, Epidermis, Fluorescent Antibody Technique, Microtubules, Blotting, Western, Keratinocytes, Tight Junctions, Adherens Junctions, Myosin Type II, Immunoprecipitation, Mice
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