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Kinesin-1–syntaphilin coupling mediates activity-dependent regulation of axonal mitochondrial transport
Open accessAbstract
Axonal mitochondria are recruited to synaptic terminals in response to neuronal activity, but the mechanisms underlying activity-dependent regulation of mitochondrial transport are largely unknown. In this paper, using genetic mouse model combined with live imaging, we demonstrate that syntaphilin (SNPH) mediates the activity-dependent immobilization of axonal mitochondria through binding to KIF5. In vitro analysis showed that the KIF5–SNPH coupling inhibited the motor adenosine triphosphatase. Neuronal activity further recruited SNPH to axonal mitochondria. This motor-docking interplay was induced by Ca2+ and synaptic activity and was necessary to establish an appropriate balance between motile and stationary axonal mitochondria. Deleting snph abolished the activity-dependent immobilization of axonal mitochondria. We propose an “Engine-Switch and Brake” model, in which SNPH acts both as an engine off switch by sensing mitochondrial Rho guanosine triphosphatase-Ca2+ and as a brake by anchoring mitochondria to the microtubule track. Altogether, our study provides new mechanistic insight into the molecular interplay between motor and docking proteins, which arrests axonal mitochondrial transport in response to changes in neuronal activity.
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“it has been largely described that Ca2+ is a key regulator of mitochondria motility and docking44–49,107.”
From CYFIP1 governs the development of cortical axons by modulating calcium availability (2025) · cites this paper as [44]
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Metadata
- Authors
- Chen, Y., & Sheng, Z.-H.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Sheng, Zu-Hang
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2013-07-15
- Volume
- 202
- Issue
- 2
- Pages
- 351-364
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC3718985
- OpenAlex
- W2059473497
- Cited by
- 250
- References
- 48
- Citations by year
- 2026: 15 · 2025: 18 · 2024: 15 · 2023: 19 · 2022: 16 · 2021: 28 · 2020: 25 · 2019: 18 · 2018: 13 · 2017: 24 · 2016: 17 · 2015: 18 · 2014: 20 · 2013: 4
- FWCI
- 8.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- syntaphilin, KIF5, kinesin-1, activity-dependent regulation, synaptic activity, calcium signaling, Rho GTPases, microtubule anchoring, synaptic mitochondria
- MeSH terms
- Animals, Axons, Biological Transport, Calcium, Carrier Proteins, Chlorocebus aethiops, Humans, Membrane Proteins, Mice, Inbred C57BL, Microtubule-Associated Proteins, Mitochondria, Nerve Tissue Proteins, Synaptic Transmission, Protein Binding, Kinesins, COS Cells, rho GTP-Binding Proteins, Protein Interaction Mapping, Mitochondrial Membranes, Mice, HEK293 Cells
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