The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
mito-QC illuminates mitophagy and mitochondrial architecture in vivo
Open accessAbstract
Autophagic turnover of mitochondria, termed mitophagy, is proposed to be an essential quality-control (QC) mechanism of pathophysiological relevance in mammals. However, if and how mitophagy proceeds within specific cellular subtypes in vivo remains unclear, largely because of a lack of tractable tools and models. To address this, we have developed “mito-QC,” a transgenic mouse with a pH-sensitive fluorescent mitochondrial signal. This allows the assessment of mitophagy and mitochondrial architecture in vivo. Using confocal microscopy, we demonstrate that mito-QC is compatible with classical and contemporary techniques in histochemistry and allows unambiguous in vivo detection of mitophagy and mitochondrial morphology at single-cell resolution within multiple organ systems. Strikingly, our model uncovers highly enriched and differential zones of mitophagy in the developing heart and within specific cells of the adult kidney. mito-QC is an experimentally advantageous tool of broad relevance to cell biology researchers within both discovery-based and translational research communities.
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Cites
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“Furthermore, our findings provoke a reassessment of how mitophagy is viewed as simply a protective response to mitotoxic damage or that it only occurs in specific physiological contexts such as reticulocyte development (Schweers et al., 2007; Mortensen et al., 2010; Novak et al., 2010).”
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Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationMitophagy and Quality Control Mechanisms in Mitochondrial Maintenance
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Mitochondrial Dynamics and Its Involvement in Disease
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Monitoring and Measuring Autophagy
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The mammalian ULK1 complex and autophagy initiation
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Metadata
- Authors
- McWilliams, T. G., Prescott, A. R., Allen, G. F. G., Tamjar, J., Munson, M. J., Thomson, C., Muqit, M. M. K., & Ganley, I. G.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2016-07-25
- Volume
- 214
- Issue
- 3
- Pages
- 333-345
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2477472785
- Cited by
- 538
- References
- 40
- FWCI
- 29.71
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- McWilliams, T. G., Prescott, A. R., Allen, G. F. G., Tamjar, J., Munson, M. J., Thomson, C., Muqit, M. M. K., & Ganley, I. G. (2016). mito-QC illuminates mitophagy and mitochondrial architecture in vivo. Journal of Cell Biology. https://doi.org/10.1083/jcb.201603039
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