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Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition

Marek, Magdalena, Vincenzetti, Vincent, Martin, Sophie G.·2020·Journal of Cell Biology
DOI 10.1083/jcb.202001147PMC PMC7265315

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Abstract

Sterols are crucial components of biological membranes, which are synthetized in the ER and accumulate in the plasma membrane (PM). Here, by applying a genetically encoded sterol biosensor (D4H), we visualize a sterol flow between PM and endosomes in the fission yeast Schizosaccharomyces pombe. Using time-lapse and correlative light-electron microscopy, we found that inhibition of Arp2/3-dependent F-actin assembly promotes the reversible relocalization of D4H from the PM to internal sterol-rich compartments (STRIC) labeled by synaptobrevin Syb1. Retrograde sterol internalization to STRIC is independent of endocytosis or an intact Golgi, but depends on Ltc1, a LAM/StARkin-family protein localized to ER-PM contact sites. The PM in ltc1Δ cells over-accumulates sterols and upon Arp2/3 inhibition forms extended ER-interacting invaginations, indicating that sterol transfer contributes to PM size homeostasis. Anterograde sterol movement from STRIC is independent of canonical vesicular trafficking but requires Arp2/3, suggesting a novel role for this complex. Thus, transfer routes orthogonal to vesicular trafficking govern the flow of sterols in the cell.

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  • Yeast mutants that lack GRAMD1 homologs (Lam/Ltc proteins) are highly susceptible to treatment with the polyene antibiotic Amphotericin B compared to WT cells.

    “Yeast mutants that lack GRAMD1 homologs (Lam/Ltc proteins) are highly susceptible to treatment with the polyene antibiotic, Amphotericin B, compared to WT cells (Gatta et al, 2015; Marek et al, 2020).”

    From Molecular basis of accessible plasma membrane cholesterol recognition by the GRAM domain of GRAMD1b (2021) · cites this paper as Marek et al, 2020

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Metadata

Authors
Marek, M., Vincenzetti, V., & Martin, S. G.
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2020-04-22
Volume
219
Issue
6
ISSN
  • 0021-9525
  • 1540-8140
DOI
10.1083/jcb.202001147
PMC
PMC7265315
Cited by
50
References
2
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
  • European Research Council
  • Swiss National Science Foundation (310030B_176396)

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