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Actin-dependent endosomal receptor recycling
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SNX17-dependent recycling requires the Retriever complex, the CCC complex, and the actin-regulatory WASH complex.
“SNX17-dependent recycling requires the Retriever complex (VPS35L, VPS26C, and VPS29), the CCC complex (COMMD/CCDC22/CCDC93), and the actin-regulatory WASH complex”
From PI(3)P coordinates SNX17- and SNX27-dependent protein recycling for long-term synaptic plasticity (2025) · cites this paper as Simonetti and Cullen, 2019
The Introduction (p. 3) identifies Retriever and CCC as regulators of SNX17-associated cargo recycling, and Figure 2 (p. 21) says Retriever binds SNX17 and associates with CCC and WASH. The paper describes these relationships but does not establish that all three complexes are required for SNX17-dependent recycling; Figure 2 also names two Retriever subunits as C16orf62 and DSCR3 rather than the aliases in the citing sentence. (LLM, conf=0.85)
Experimental — accuracy not yet measuredEndosomal recycling by Commander requires dynamic organization of actin-rich domains on the endosomal surface.
“Endosomal recycling by Commander requires dynamic organization of actin-rich domains on the endosomal surface.4”
From Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome (2023) · cites this paper as [4]
The Figure 4 caption (p. 23) says regulated receptor recycling depends on actin dynamics at the endosomal surface and involves actin-enriched subdomains. The section “Actin and WASH in the organization of the retrieval subdomains” (p. 6) links WASH-mediated domain organization to the retriever complex via the CCC complex. The paper does not name Commander or establish that recycling by Commander specifically requires this organization. (LLM, conf=0.83)
Experimental — accuracy not yet measuredEndosome-associated branched actin is nucleated by Arp2/3 following activation by the WASH complex, which interacts with Commander subunits.
“Endosome-associated branched actin is nucleated by Arp2/3 following activation by the WASH complex, which interacts with Commander subunits.4,6,13,21,65,78”
From Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome (2023) · cites this paper as [4]
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Retromer: A Master Conductor of Endosome Sorting
Burd, C., & Cullen, P. J. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a016774 · Cited by 435
Self-citationRetriever is a multiprotein complex for retromer-independent endosomal cargo recycling
McNally, K. E., Faulkner, R., Steinberg, F., Gallon, M., Ghai, R., Pim, D., Langton, P., Pearson, N., Danson, C. M., Nägele, H., et al. (2017) · Nature Cell Biology · doi:10.1038/ncb3610 · Cited by 346
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Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome
Healy, M. D., McNally, K. E., Butkovič, R., Chilton, M., Kato, K., Sacharz, J., McConville, C., Moody, E. R. R., Shaw, S., Planelles-Herrero, V. J., et al. (2023) · Cell · doi:10.1016/j.cell.2023.04.003 · Cited by 91
Self-citationRecruitment of the SNX17-Retriever recycling pathway regulates synaptic function and plasticity
Rivero-Ríos, P., Tsukahara, T., Uygun, T., Chen, A., Chavis, G. D., Giridharan, S. S. P., Iwase, S., Sutton, M. A., & Weisman, L. S. (2023) · Journal of Cell Biology · doi:10.1083/jcb.202207025 · Cited by 17
Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation
Antón-Plágaro, C., Chen, K.-E., Guo, Q., Liu, M., Evans, A. J., Lewis, P. A., Heesom, K. J., Wilkinson, K. A., Collins, B. M., & Cullen, P. J. (2025) · Nature Communications · doi:10.1038/s41467-025-61802-1 · Cited by 14
Self-citationPI(3)P coordinates SNX17- and SNX27-dependent protein recycling for long-term synaptic plasticity
Rivero-Ríos, P., Uygun, T., Chavis, G. D., Lee, H., Duan, B., Sutton, M. A., & Weisman, L. S. (2025) · Journal of Cell Biology · doi:10.1083/jcb.202411198 · Cited by 1
Chavis, G. D., Rivero-Ríos, P., Uygun, T., Tsukahara, T., Hayami, T., Lee, G. Y., Lee, M., Pace, C., Czesak, F. N., Hilde, K. L., et al. (2025) · Neuroscience · doi:10.1016/j.neuroscience.2025.11.009
Metadata
- Authors
- Simonetti, B., & Cullen, P. J.
- Venue
- Current Opinion in Cell Biology
- Publisher
- Elsevier BV
- Published
- 2019-02
- Volume
- 56
- Pages
- 22-33
- ISSN
- 0955-0674
- Cited by
- 139
- References
- 2
- Language
- en
- Licence
- Funders
- MRC (MR/L007363/1, MR/P018807/1)
- Wellcome Trust (104568/Z/14/2)
- Wellcome Trust PhD Studentship (083474)
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