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Cited by 11Cellular transport and secretionIon Channels and ReceptorsLipid Membrane Structure and Behavior

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A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts

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Johnson, Ben, Iuliano, Maria, Lam, TuKiet T., Biederer, Thomas, De Camilli, Pietro V.·2024·Journal of Cell Biology
DOI 10.1083/jcb.202311137PMC PMC11334333

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Abstract

Junctions between the ER and plasma membrane (PM) are implicated in calcium homeostasis, non-vesicular lipid transfer, and other cellular functions. Two ER proteins that function both as tethers to the PM via a polybasic C-terminus motif and as phospholipid transporters are brain-enriched TMEM24 (C2CD2L) and its paralog C2CD2. We report that both proteins also form a complex with band 4.1 family members, which in turn bind PM proteins including cell adhesion molecules such as SynCAM 1. This complex enriches TMEM24 and C2CD2 containing ER/PM junctions at sites of cell contacts. Dynamic properties of TMEM24-dependent ER/PM junctions are impacted when band 4.1 is part of the junction, as TMEM24 at cell-adjacent ER/PM junctions is not shed from the PM by calcium rise, unlike TMEM24 at non-cell adjacent junctions. Lipid transport between the ER and the PM by TMEM24 and C2CD2 at sites where cells, including neurons, contact other cells may participate in adaptive responses to cell contact-dependent signaling.

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Metadata

Authors
Johnson, B., Iuliano, M., Lam, T. T., Biederer, T., & De Camilli, P. V.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Tufts University (US)
  • Yale University (US)
  • Yale University School of Medicine 1 Department of Neuroscience, , New Haven, CT, USA
ORCID
Venue
Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2024-08-19
Volume
223
Issue
11
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.202311137
PMC
PMC11334333
OpenAlex
W4401700569
Cited by
11
References
3
Citations by year
2026: 3 · 2025: 6 · 2024: 1
FWCI
2.24
Type
journal article
Language
en
Open access
Licence
Locations
  • The Journal of Cell Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion, cc-by)
Funders
  • National Institutes of Health (R01 NS036251, DA018343, R01 DA018928, S10OD02365101A1, S10OD019967, S10OD018034)
  • Kavli Institute for Neuroscience
  • Yale School of Medicine
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
Keywords
band 4.1, ER-plasma membrane junctions, lipid transport, cell-cell contact, calcium homeostasis, protein complex formation, neurons

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