Expression of adhesion molecules and chemotactic cytokines in cultured human mesothelial cells.
Open accessAbstract
The mesothelium is a flat epithelial lining of serous cavities that could gate the traffic of molecules and cells between the circulation and these body compartments. The present study was designed to elucidate the capacity of mesothelial cells to express adhesion molecules and chemoattractant cytokines, two fundamental mechanisms of regulation of leukocyte recruitment. Cultured human mesothelial cells express appreciable levels of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1), and these were increased by in vitro exposure to tumor necrosis factor (TNF), interferon gamma (IFN-gamma), or TNF and IFN-gamma. Interleukin 1 (IL-1) was a less consistent stimulus for adhesion molecule expression in vitro. Unlike endothelial cells, used as a reference cell population, resting or stimulated mesothelial cells did not express E-selectin and ICAM-2, as assessed by flow cytometry. Analysis of VCAM-1 mRNA by reverse transcriptase and polymerase chain reaction using appropriate primers revealed that mesothelial cells expressed both the seven- and the six-Ig domain transcripts, with predominance of the longer species. Monocytes bound appreciably to "resting" and, to a greater extent, to stimulated mesothelial cells. Monocytes exposed to IFN-gamma and lipopolysaccharide, used as prototypic activation signals, showed increased capacity to bind mesothelial cells. Anti-CD18 monoclonal antibody significantly inhibited binding of monocytes to mesothelial cells, and this blocking effect was amplified by anti-very late antigen 4. Mesothelial cells were able to express the chemotactic cytokines IL-8 and monocyte chemotactic protein 1 at the mRNA and protein levels. These results indicate that mesothelial cells can express a set of adhesion molecules (ICAM-1 and VCAM-1) overlapping with, but distinct from, that expressed in vascular endothelium (ICAM-1, ICAM-2, VCAM-1, E-selectin), and that these are functionally relevant for interacting with mononuclear phagocytes. The regulated expression of adhesion molecules and chemotactic cytokines by mesothelial cells is probably important in inflammatory and immune reactions that involve serous cavities, such as the long-known macrophage appearance and disappearance reactions.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
TAMs induce VCAM-1 expression on mesothelial cells, a step believed key for tumour cell dissemination into the peritoneum.
“TAMs also induce VCAM-1 expression on mesothelial cells, a step also believed to be key for tumour cell dissemination into the peritoneum20.”
From Inflammation and cancer (2002) · cites this paper as [20]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,725 · see citing works
Adhesion receptors of the immune system
Springer, T. A. (1990) · Nature · doi:10.1038/346425a0 · Cited by 6,477 · see citing works
Leukocyte-endothelial cell recognition: Three (or more) steps to specificity and diversity
Butcher, E. C. (1991) · Cell · doi:10.1016/0092-8674(91)90279-8 · Cited by 2,789 · see citing works
Lawrence, M. B., & Springer, T. A. (1991) · Cell · doi:10.1016/0092-8674(91)90393-d · Cited by 2,157 · see citing works
Properties Of The Novel Proinflammatory Supergene Intercrine Cytokine Family
Oppenheim, J. J., Zachariae, C. O. C., Mukaida, N., & Matsushima, K. (1991) · Annual Review of Immunology · doi:10.1146/annurev.iy.09.040191.003153 · Cited by 1,878 · see citing works
Elices, M. J., Osborn, L., Takada, Y., Crouse, C., Luhowskyj, S., Hemler, M. E., & Lobb, R. R. (1990) · Cell · doi:10.1016/0092-8674(90)90661-w · Cited by 1,808 · see citing works
Endothelial Cell Gene Expression of a Neutrophil Chemotactic Factor by TNF-α, LPS, and IL-1β
Strieter, R. M., Kunkel, S. L., Showell, H. J., Remick, D. G., Phan, S. H., Ward, P. A., & Marks, R. M. (1989) · Science · doi:10.1126/science.2648570 · Cited by 775 · see citing works
Cytokine regulation of endothelial cell function
Mantovani, A., Bussolino, F., & Dejana, E. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.8.1592209 · Cited by 677 · see citing works
Self-citationAn Inducible Endothelial Cell Surface Glycoprotein Mediates Melanoma Adhesion
Rice, G. E., & Bevilacqua, M. P. (1989) · Science · doi:10.1126/science.2588007 · Cited by 650 · see citing works
IL-1 stimulates IL-6 production in endothelial cells.
Sironi, M., Breviario, F., Proserpio, P., Biondi, A., Vecchi, A., Damme, J. V., Dejana, E., & Mantovani, A. (1989) · The Journal of Immunology · doi:10.4049/jimmunol.142.2.549 · Cited by 522 · see citing works
Self-citationWu, Y.-J., Parker, L. M., Binder, N., Beckett, M. A., Sinard, J. H., Griffiths, C. T., & Rheinwald, J. G. (1982) · Cell · doi:10.1016/0092-8674(82)90324-5 · Cited by 513 · see citing works
Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
de Fougerolles, A. R., Stacker, S. A., Schwarting, R., & Springer, T. A. (1991) · The Journal of Experimental Medicine · doi:10.1084/jem.174.1.253 · Cited by 495 · see citing works
Rollins, B. J., Yoshimura, T., Leonard, E. J., & Pober, J. S. (1990) · Europe PMC (PubMed Central) · Cited by 492 · see citing works
Dixit, V. M., Green, S., Sarma, V. J., Holzman, L. B., Wolf, F. W., O’Rourke, K. M., Ward, P. A., Prochownik, E. V., & Marks, R. M. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39896-5 · Cited by 375 · see citing works
Effect of venous shear stress on CD18-mediated neutrophil adhesion to cultured endothelium
Lawrence, M., Smith, C., Eskin, S. G., et al. (1990) · Blood · doi:10.1182/blood.v75.1.227.227 · Cited by 357 · see citing works
Sica, A., Wang, J. M., Colotta, F., Dejana, E., Mantovani, A., Oppenheim, J. J., Larsen, C. G., Zachariae, C. O. C., & Matsushima, K. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.144.8.3034 · Cited by 343 · see citing works
Self-citationCarlos, T. M., Kovach, N. L., Schwartz, B. R., Rosa, M., Newman, B., Wayner, E. A., Benjamin, C. D., Osborn, L., Lobb, R. R., & Harlan, J. (1991) · Blood · doi:10.1182/blood.v77.10.2266.2266 · Cited by 318 · see citing works
Colotta, F., Peri, G., Villa, A., & Mantovani, A. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.132.2.936 · Cited by 261 · see citing works
Self-citationInterleukin 1-induced augmentation of experimental metastases from a human melanoma in nude mice.
Giavazzi, R., Garofalo, A., Bani, M. R., Abbate, M., Ghezzi, P., Boraschi, D., Mantovani, A., & Dejana, E. (1990) · PubMed · Cited by 201 · see citing works
Self-citationSchröder, J. M., & Christophers, E. (1989) · The Journal of Immunology · doi:10.4049/jimmunol.142.1.244 · Cited by 182 · see citing works
IL-1 transcriptionally activates the neutrophil chemotactic factor/IL-8 gene in endothelial cells.
Sica, A., Matsushima, K., Damme, J. V., Wang, J. M., Polentarutti, N., Dejana, E., Colotta, F., & Mantovani, A. (1990) · PubMed Central · Cited by 165 · see citing works
Self-citationMolecules and structures involved in the adhesion of natural killer cells to vascular endothelium.
Allavena, P., Paganin, C., Martín-Padura, I., et al. (1991) · The Journal of Experimental Medicine · doi:10.1084/jem.173.2.439 · Cited by 161 · see citing works
Demetri, G. D., Zenzie, B. W., Rheinwald, J. G., & Griffin, J. D. (1989) · Blood · doi:10.1182/blood.v74.3.940.940 · Cited by 145 · see citing works
Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells.
Dejana, E., Bertocchi, F., Bortolami, M., Regonesi, A., Tonta, A., Breviario, F., & Giavazzi, R. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113753 · Cited by 145 · see citing works
Doherty, D. E., Haslett, C., Tonnesen, M. G., & Henson, P. M. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.6.1762 · Cited by 125 · see citing works
Alternative splicing of human VCAM-1 in activated vascular endothelium.
Cybulsky, M. I., Fries, J. W. U., Williams, A. J., et al. (1991) · PubMed · Cited by 123 · see citing works
Cloning of an alternate form of vascular cell adhesion molecule-1 (VCAM1).
Hession, C. A., Tizard, R., Vassallo, C., et al. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)89551-9 · Cited by 123 · see citing works
Nelson, D. S., & BOYDEN, S. V. (1963) · PubMed · Cited by 106 · see citing works
Nortamo, P., Salcedo, R., Timonen, T. T., et al. (1991) · The Journal of Immunology · doi:10.4049/jimmunol.146.8.2530 · Cited by 92 · see citing works
Elliott, M. J., Vadas, M. A., Cleland, L. G., et al. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.145.1.167 · Cited by 74 · see citing works
Molecules involved in the adhesion and cytotoxicity of activated monocytes on endothelial cells
Jonjić, N., Jílek, P., Bernasconi, S. M., et al. (1992) · The Journal of Immunology · doi:10.4049/jimmunol.148.7.2080 · Cited by 67 · see citing works
Self-citationCytotoxicity of activated monocytes on endothelial cells.
Péri, G., Chiaffarino, F., Bernasconi, S. M., et al. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.144.4.1444 · Cited by 57 · see citing works
Self-citationThe macrophage disappearance reaction: Mediation by a soluble lymphocyte-derived factor
Sonozaki, H., & Cohen, S. (1971) · Cellular Immunology · doi:10.1016/0008-8749(71)90069-4 · Cited by 47 · see citing works
Differential responses to growth factors by normal human mesothelial cultures from individual donors
Lechner, J. F., LaVeck, M. A., Gerwin, B. I., et al. (1989) · Journal of Cellular Physiology · doi:10.1002/jcp.1041390211 · Cited by 35 · see citing works
Inhibition of human monocyte adhesion by interleukin-4
Elliott, M., Gamble, J. R., Park, L., et al. (1991) · Blood · doi:10.1182/blood.v77.12.2739.2739 · Cited by 34 · see citing works
Sonozaki, H., Papermaster, V., Yoshida, T., et al. (1975) · The Journal of Immunology · doi:10.4049/jimmunol.115.6.1657 · Cited by 32 · see citing works
Marker profile of mesothelial cells versus ovarian carcinoma cells
van Niekerk, C. C., Jap, P. H. K., Thomas, C. M. G., et al. (1989) · International Journal of Cancer · doi:10.1002/ijc.2910430619 · Cited by 32 · see citing works
The Effect of Sensitized Lymphocytes on Peritoneal Exudate Macrophages in the Guinea Pig
Sonozaki, H., & Cohen, S. (1971) · The Journal of Immunology · doi:10.4049/jimmunol.106.5.1404 · Cited by 19 · see citing works
The effect of lymphokines on macrophage accumulation and disappearance in the peritoneal cavity.
D’Silva, H., Yoshida, T., & Cohen, S. (1983) · PubMed · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Coussens, L. M., & Werb, Z. (2002) · Nature · doi:10.1038/nature01322 · Cited by 15,111 · see citing works
McLoughlin, R. M., Witowski, J., Robson, R. L., Wilkinson, T. S., Hurst, S. M., Williams, A. S., Williams, J. D., Rose-John, S., Jones, S. A., & Topley, N. (2003) · Journal of Clinical Investigation · doi:10.1172/jci17129 · Cited by 281 · see citing works
Tumors and inflammatory infiltrates: Friends or foes?
Brigati, C., Noonan, D. M., Albini, A., & Benelli, R. (2002) · Clinical & Experimental Metastasis · doi:10.1023/a:1015587423262 · Cited by 230 · see citing works
Dietary Oleic Acid Increases M2 Macrophages in the Mesenteric Adipose Tissue
Camell, C., & Smith, C. W. (2013) · PLoS ONE · doi:10.1371/journal.pone.0075147 · Cited by 76 · see citing works
Pathogenesis of endometriosis--current research
Witz, C. A., Allsup, K. T., Montoya-Rodriguez, I. A., Vaughan, S. L., Centonze, V. E., & Schenken, R. S. (2003) · Human Fertility · doi:10.1080/1464770312331368973 · Cited by 24 · see citing works
- Authors
- Jonjić, N., Peri, G., Bernasconi, S., Sciacca, F. L., Colotta, F., Pelicci, P., Lanfrancone, L., & Mantovani, A.
- Affiliations
- Mario Negri Institute for Pharmacological Research (IT)
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1992-10-01
- Volume
- 176
- Issue
- 4
- Pages
- 1165-1174
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2119405
- OpenAlex
- W1980790429
- Cited by
- 290
- References
- 39
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 8 · 2022: 6 · 2021: 11 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 7 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 7
- FWCI
- 4.43
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
- Keywords
- mesothelial cells, adhesion molecules, chemokines, ICAM-1, VCAM-1, monocyte adhesion, leukocyte recruitment, TNF-α, interferon-gamma, interleukin-1, interleukin-8, MCP-1, CD18, VLA-4, serous cavities, inflammation
- MeSH terms
- Base Sequence, Cell Adhesion, Cells, Cultured, Chemotaxis, Epithelial Cells, Epithelium, Humans, Leukocytes, Molecular Sequence Data, Monocytes, Oligodeoxyribonucleotides, RNA, Antigens, CD, Cell Adhesion Molecules, Polymerase Chain Reaction, Cytokines, Vascular Cell Adhesion Molecule-1, E-Selectin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
