The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Different responses are elicited in cytotoxic T lymphocytes by different levels of T cell receptor occupancy.
Open accessAbstract
We have investigated the level of TCR occupancy required to elicit different biological responses in human CTL clones specific for an influenza matrix peptide. Specific cytotoxicity could be detected at extremely low peptide concentrations (10(-12) to 10(-15) M). However, IFN-gamma production, responsiveness to IL-2 and Ca++ fluxes were observed only at peptide concentrations > 10(-9) M, while autonomous proliferation required even higher peptide concentrations. In parallel experiments we measured TCR downregulation to estimate the number of TCRs triggered. We observed that at low peptide concentrations, where only cytotoxicity is triggered, TCR downregulation was hardly detectable. Conversely, induction of IFN-gamma production and proliferation required triggering of at least 20-50% of TCRs. Taken together these results indicate that a single CTL can graduate different biological responses as a function of antigen concentration and that killing of the specific target does not necessarily result in full activation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Moskophidis, D., Lechner, F., Pircher, H., & Zinkernagel, R. M. (1993) · Nature · doi:10.1038/362758a0 · Cited by 1,240
Serial triggering of many T-cell receptors by a few peptide–MHC complexes
Valitutti, S., Müller, S., Cella, M., Padovan, E., & Lanzavecchia, A. (1995) · Nature · doi:10.1038/375148a0 · Cited by 1,128
Self-citationValitutti, S., Dessing, M., Aktories, K., Gallati, H., & Lanzavecchia, A. (1995) · The Journal of experimental medicine · doi:10.1084/jem.181.2.577 · Cited by 531
Self-citationHarding, C. V., & Unanue, E. R. (1990) · Nature · doi:10.1038/346574a0 · Cited by 453
The Minimal Number of Class II MHC-Antigen Complexes Needed for T Cell Activation
Demotz, S., Grey, H. M., & Sette, A. (1990) · Science · doi:10.1126/science.2118680 · Cited by 400
Roles Of Alpha-Beta And Gamma-Delta T-Cell Subsets In Viral Immunity
Doherty, P. (1992) · Annual Review of Immunology · doi:10.1146/annurev.immunol.10.1.123 · Cited by 354
Enhanced establishment of a virus carrier state in adult CD4+ T-cell-deficient mice
Battegay, M., Moskophidis, D., Rahemtulla, A., Hengartner, H., Mak, T. W., & Zinkernagel, R. M. (1994) · Journal of Virology · doi:10.1128/jvi.68.7.4700-4704.1994 · Cited by 320
Direct activation of CD8+ cytotoxic T lymphocytes by dendritic cells.
Inaba, K., Young, J. W., & Steinman, R. M. (1987) · The Journal of experimental medicine · doi:10.1084/jem.166.1.182 · Cited by 240
Kageyama, S., Tsomides, T. J., Sykulev, Y. K., & Eisen, H. N. (1995) · The Journal of Immunology · doi:10.4049/jimmunol.154.2.567 · Cited by 143
Gotch, F., McMichael, A. J., & Rothbard, J. B. (1988) · The Journal of Experimental Medicine · doi:10.1084/jem.168.6.2045 · Cited by 114
Cited by
HIV-specific CD8(+) T cells produce antiviral cytokines but are impaired in cytolytic function
Appay, V., Nixon, D. F., Donahoe, S. M., Gillespie, G. M. A., Dong, T., King, A., Ogg, G. S., Spiegel, H. M. L., Conlon, C., Spina, C. A., et al. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.192.1.63 · Cited by 871
Regulatory T cells suppress tumor-specific CD8 T cell cytotoxicity through TGF-β signals in vivo
Chen, M.-L., Pittet, M. J., Gorelik, L., Flavell, R. A., Weissleder, R., von Boehmer, H., & Khazaie, K. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408197102 · Cited by 806
The Critical Need for CD4 Help in Maintaining Effective Cytotoxic T Lymphocyte Responses
Kalams, S. A., & Walker, B. D. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.12.2199 · Cited by 660
CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics
van der Merwe, P. A., Bodian, D. L., Daenke, S., Linsley, P., & Davis, S. J. (1997) · The Journal of Experimental Medicine · doi:10.1084/jem.185.3.393 · Cited by 551
T Cell Affinity Maturation by Selective Expansion during Infection
Busch, D. H., & Pamer, E. G. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.4.701 · Cited by 406
Metadata
- Authors
- Valitutti, S., Müller, S., Dessing, M., & Lanzavecchia, A.
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1996-04-01
- Volume
- 183
- Issue
- 4
- Pages
- 1917-1921
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2132156332
- Cited by
- 435
- References
- 12
- FWCI
- 11.81
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Different responses are elicited in cytotoxic T lymphocytes by different levels of T cell receptor occupancy. https://app.cochat.ai/papers/10.1084/jem.183.4.1917
- The paper
- Valitutti, S., Müller, S., Dessing, M., & Lanzavecchia, A. (1996). Different responses are elicited in cytotoxic T lymphocytes by different levels of T cell receptor occupancy. The Journal of experimental medicine. https://doi.org/10.1084/jem.183.4.1917
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
