The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
pH-dependent Perforation of Macrophage Phagosomes by Listeriolysin O from Listeria monocytogenes
Open accessAbstract
The pore-forming toxin listeriolysin O (LLO) is a major virulence factor implicated in escape of Listeria monocytogenes from phagocytic vacuoles. Here we describe the pH-dependence of vacuolar perforation by LLO, using the membrane-impermeant fluorophore 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) to monitor the pH and integrity of vacuoles in mouse bone marrow–derived macrophages. Perforation was observed when acidic vacuoles containing wild-type L. monocytogenes displayed sudden increases in pH and release of HPTS into the cytosol. These changes were not seen with LLO-deficient mutants. Perforation occurred at acidic vacuolar pH (4.9–6.7) and was reduced in frequency or prevented completely when macrophages were treated with the lysosomotropic agents ammonium chloride or bafilomycin A1. We conclude that acidic pH facilitates LLO activity in vivo.
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Cited by
Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH
Cotter, P. D., & Hill, C. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.3.429-453.2003 · Cited by 1,379
Antimicrobial mechanisms of phagocytes and bacterial evasion strategies
Flannagan, R. S., Cosío, G., & Grinstein, S. (2009) · Nature Reviews Microbiology · doi:10.1038/nrmicro2128 · Cited by 947
Apoptosis facilitates antigen presentation to T lymphocytes through MHC-I and CD1 in tuberculosis
Schaible, U. E., Winau, F., Sieling, P. A., Fischer, K., Collins, H. L., Hagens, K., Modlin, R. L., Brinkmann, V., & Kaufmann, S. H. E. (2003) · Nature Medicine · doi:10.1038/nm906 · Cited by 546
Current status of pH-sensitive liposomes in drug delivery
Drummond, D. C., Zignani, M., & Leroux, J.-C. (2000) · Progress in Lipid Research · doi:10.1016/s0163-7827(00)00011-4 · Cited by 477
Ito Cells Are Liver-Resident Antigen-Presenting Cells for Activating T Cell Responses
Winau, F., Hegasy, G., Weiskirchen, R., Weber, S., Cassan, C., Sieling, P. A., Modlin, R. L., Liblau, R. S., Gressner, A. M., & Kaufmann, S. H. E. (2007) · Immunity · doi:10.1016/j.immuni.2006.11.011 · Cited by 410
Metadata
- Authors
- Beauregard, K. E., Lee, K.-D., Collier, R. J., & Swanson, J. A.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1997-10-06
- Volume
- 186
- Issue
- 7
- Pages
- 1159-1163
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2037559791
- Cited by
- 260
- References
- 29
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: pH-dependent Perforation of Macrophage Phagosomes by Listeriolysin O from Listeria monocytogenes. https://app.cochat.ai/papers/10.1084/jem.186.7.1159
- The paper
- Beauregard, K. E., Lee, K.-D., Collier, R. J., & Swanson, J. A. (1997). pH-dependent Perforation of Macrophage Phagosomes by Listeriolysin O from Listeria monocytogenes. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.186.7.1159
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
