Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 822

The DOI matches its Crossref record.

Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells

Open access
Bachem, Annabell, Güttler, Steffen, Hartung, Evelyn, Ebstein, Frédéric, Schaefer, Michael, Tannert, Astrid, Salama, Abdulgabar, Movassaghi, Kamran, Opitz, Corinna, Mages, Hans W., Henn, Volker, Kloetzel, Peter-Michael, Gurka, Stephanie, Kroczek, Richard A.·2010·Journal of Experimental Medicine
DOI 10.1084/jem.20100348
Open this paper in CoChat — free account

Abstract

In recent years, human dendritic cells (DCs) could be subdivided into CD304+ plasmacytoid DCs (pDCs) and conventional DCs (cDCs), the latter encompassing the CD1c+, CD16+, and CD141+ DC subsets. To date, the low frequency of these DCs in human blood has essentially prevented functional studies defining their specific contribution to antigen presentation. We have established a protocol for an effective isolation of pDC and cDC subsets to high purity. Using this approach, we show that CD141+ DCs are the only cells in human blood that express the chemokine receptor XCR1 and respond to the specific ligand XCL1 by Ca2+ mobilization and potent chemotaxis. More importantly, we demonstrate that CD141+ DCs excel in cross-presentation of soluble or cell-associated antigen to CD8+ T cells when directly compared with CD1c+ DCs, CD16+ DCs, and pDCs from the same donors. Both in their functional XCR1 expression and their effective processing and presentation of exogenous antigen in the context of major histocompatibility complex class I, human CD141+ DCs correspond to mouse CD8+ DCs, a subset known for superior antigen cross-presentation in vivo. These data define CD141+ DCs as professional antigen cross-presenting DCs in the human.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1084/jem.20100348

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 36

  1. Andrews, 2008, Cancer vaccines for established cancer: how to make them better?, Immunol. Rev., 222, 242, 10.1111/j.1600-065X.2008.00612.x · DOI
  2. Bevan, 2006, Cross-priming, Nat. Immunol., 7, 363, 10.1038/ni0406-363 · DOI
  3. Caminschi, 2008, The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement, Blood., 112, 3264, 10.1182/blood-2008-05-155176 · DOI · Paper page
  4. Caminschi, 2009, Enhancing immune responses by targeting antigen to DC, Eur. J. Immunol., 39, 931, 10.1002/eji.200839035 · DOI
  5. Colonna, 2004, Plasmacytoid dendritic cells in immunity, Nat. Immunol., 5, 1219, 10.1038/ni1141 · DOI · Paper page
  6. den Haan, 2000, CD8+ but not CD8− dendritic cells cross-prime cytotoxic T cells in vivo, J. Exp. Med., 192, 1685, 10.1084/jem.192.12.1685 · DOI · Paper page
  7. Di Pucchio, 2008, Direct proteasome-independent cross-presentation of viral antigen by plasmacytoid dendritic cells on major histocompatibility complex class I, Nat. Immunol., 9, 551, 10.1038/ni.1602 · DOI · Paper page
  8. Dorner, 2002, MIP-1α, MIP-1β, RANTES, and ATAC/lymphotactin function together with IFN-γ as type 1 cytokines, Proc. Natl. Acad. Sci. USA., 99, 6181, 10.1073/pnas.092141999 · DOI · Paper page
  9. Dorner, 2009, Selective expression of the chemokine receptor XCR1 on cross-presenting dendritic cells determines cooperation with CD8+ T cells, Immunity., 31, 823, 10.1016/j.immuni.2009.08.027 · DOI · Paper page
  10. Dzionek, 2000, BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood, J. Immunol., 165, 6037, 10.4049/jimmunol.165.11.6037 · DOI · Paper page
  11. Esmon, 1982, Isolation of a membrane-bound cofactor for thrombin-catalyzed activation of protein C, J. Biol. Chem., 257, 859, 10.1016/S0021-9258(19)68276-1 · DOI · Paper page
  12. Flavell, 1995, Preclinical studies with the anti-CD19-saporin immunotoxin BU12-SAPORIN for the treatment of human-B-cell tumours, Br. J. Cancer., 72, 1373, 10.1038/bjc.1995.517 · doi:10.1038/bjc.1995.517
  13. Fonteneau, 2001, Generation of high quantities of viral and tumor-specific human CD4+ and CD8+ T-cell clones using peptide pulsed mature dendritic cells, J. Immunol. Methods., 258, 111, 10.1016/S0022-1759(01)00477-X · DOI
  14. Fonteneau, 2003, Characterization of the MHC class I cross-presentation pathway for cell-associated antigens by human dendritic cells, Blood., 102, 4448, 10.1182/blood-2003-06-1801 · DOI
  15. Galibert, 2005, Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule, J. Biol. Chem., 280, 21955, 10.1074/jbc.M502095200 · DOI · Paper page
  16. Heath, 2009, Dendritic cell subsets in primary and secondary T cell responses at body surfaces, Nat. Immunol., 10, 1237, 10.1038/ni.1822 · DOI · Paper page
  17. Heath, 2004, Cross-presentation, dendritic cell subsets, and the generation of immunity to cellular antigens, Immunol. Rev., 199, 9, 10.1111/j.0105-2896.2004.00142.x · DOI · Paper page
  18. Hoeffel, 2007, Antigen crosspresentation by human plasmacytoid dendritic cells, Immunity., 27, 481, 10.1016/j.immuni.2007.07.021 · DOI · Paper page
  19. Iyoda, 2002, The CD8+ dendritic cell subset selectively endocytoses dying cells in culture and in vivo, J. Exp. Med., 195, 1289, 10.1084/jem.20020161 · DOI · Paper page
  20. Ju, 2010, Review of human DC subtypes, Methods Mol. Biol., 595, 3, 10.1007/978-1-60761-421-0_1 · DOI
  21. Lenz, 2002, Ca2+-controlled competitive diacylglycerol binding of protein kinase C isoenzymes in living cells, J. Cell Biol., 159, 291, 10.1083/jcb.200203048 · DOI
  22. Lin, 2008, The cell biology of cross-presentation and the role of dendritic cell subsets, Immunol. Cell Biol., 86, 353, 10.1038/icb.2008.3 · DOI · Paper page
  23. Lindstedt, 2005, Gene family clustering identifies functionally associated subsets of human in vivo blood and tonsillar dendritic cells, J. Immunol., 175, 4839, 10.4049/jimmunol.175.8.4839 · DOI · Paper page
  24. Liu, 2005, IPC: professional type 1 interferon-producing cells and plasmacytoid dendritic cell precursors, Annu. Rev. Immunol., 23, 275, 10.1146/annurev.immunol.23.021704.115633 · DOI · Paper page
  25. MacDonald, 2002, Characterization of human blood dendritic cell subsets, Blood., 100, 4512, 10.1182/blood-2001-11-0097 · DOI · Paper page
  26. Piccioli, 2007, Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets, Blood., 109, 5371, 10.1182/blood-2006-08-038422 · DOI · Paper page
  27. Robbins, 2008, Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling, Genome Biol., 9, R17, 10.1186/gb-2008-9-1-r17 · DOI · Paper page
  28. Robinson, 1999, Human peripheral blood contains two distinct lineages of dendritic cells, Eur. J. Immunol., 29, 2769, 10.1002/(SICI)1521-4141(199909)29:09<2769::AID-IMMU2769>3.0.CO;2-2 · DOI · Paper page
  29. Schnurr, 2005, Tumor antigen processing and presentation depend critically on dendritic cell type and the mode of antigen delivery, Blood., 105, 2465, 10.1182/blood-2004-08-3105 · DOI · Paper page
  30. Schulz, 2002, Cross-presentation of cell-associated antigens by CD8α+ dendritic cells is attributable to their ability to internalize dead cells, Immunology., 107, 183, 10.1046/j.1365-2567.2002.01513.x · DOI · Paper page
  31. Segura, 2009, Antigen presentation by dendritic cells in vivo, Curr. Opin. Immunol., 21, 105, 10.1016/j.coi.2009.03.011 · DOI · Paper page
  32. Shen, 2006, Priming of T cells by exogenous antigen cross-presented on MHC class I molecules, Curr. Opin. Immunol., 18, 85, 10.1016/j.coi.2005.11.003 · doi:10.1016/j.coi.2005.11.003
  33. Shortman, 2010, The CD8+ dendritic cell subset, Immunol. Rev., 234, 18, 10.1111/j.0105-2896.2009.00870.x · DOI · Paper page
  34. Steinman, 2007, Taking dendritic cells into medicine, Nature., 449, 419, 10.1038/nature06175 · DOI · Paper page
  35. Villadangos, 2008, Antigen-presentation properties of plasmacytoid dendritic cells, Immunity., 29, 352, 10.1016/j.immuni.2008.09.002 · DOI · Paper page
  36. Villadangos, 2007, Outside looking in: the inner workings of the cross-presentation pathway within dendritic cells, Trends Immunol., 28, 45, 10.1016/j.it.2006.12.008 · doi:10.1016/j.it.2006.12.008

Cited by

See all 822

Metadata

Authors
Bachem, A., Güttler, S., Hartung, E., Ebstein, F., Schaefer, M., Tannert, A., Salama, A., Movassaghi, K., Opitz, C., Mages, H. W., Henn, V., Kloetzel, P.-M., Gurka, S., & Kroczek, R. A.
Venue
Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
2010-05-17
Volume
207
Issue
6
Pages
1273-1281
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.20100348
OpenAlex
W2120315525
Cited by
822
References
0
FWCI
27.05
Type
journal article
Language
en
Open access
hybrid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells. https://app.cochat.ai/papers/10.1084/jem.20100348
The paper
Bachem, A., Güttler, S., Hartung, E., Ebstein, F., Schaefer, M., Tannert, A., Salama, A., Movassaghi, K., Opitz, C., Mages, H. W., Henn, V., Kloetzel, P.-M., Gurka, S., & Kroczek, R. A. (2010). Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells. Journal of Experimental Medicine. https://doi.org/10.1084/jem.20100348

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.