Evolutionary Biology and Gene Editing of Cat Allergen, Fel d 1
Open accessAbstract
Allergy to domestic cat affects up to 15% of the population, and sensitization to cat allergen is associated with asthma. Despite the pervasiveness of cat allergic disease, current treatments have limited impact. Here, we present a bioinformatics analysis of the major cat allergen, Fel d 1, and demonstrate proof of principle for CRISPR gene editing of the allergen. Sequence and structural analyses of Fel d 1 from 50 domestic cats identified conserved coding regions in genes CH1 and CH2 suitable for CRISPR editing. Comparative analyses of Fel d 1 and orthologous sequences from eight exotic felid species determined relatively low-sequence identities for CH1 and CH2 , and implied that the allergen may be nonessential for cats, given the apparent lack of evolutionary conservation. In vitro knockouts of domestic cat Fel d 1 using CRISPR-Cas9 yielded editing efficiencies of up to 55% and found no evidence of editing at predicted potential off-target sites. Taken together, our data indicate that Fel d 1 is both a rational and viable candidate for gene deletion, which may profoundly benefit cat allergy sufferers by removing the major allergen at the source.
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Using CRISPR-Cas9, exons CH1 and CH2 were deleted in CRFK cells with an efficiency of 55%.
“Using CRISPR-Cas9, exons CH1 and CH2 were deleted in CRFK cells with an efficiency of 55% [119,120].”
From Advance in Managing Indoor Cat Allergen Proteins: Molecular Insights, Detection, and Control (2025) · cites this paper as [119]
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- Authors
- Brackett, N. F., Davis, B. W., Adli, M., Pomés, A., & Chapman, M. D.
- Affiliations
- Northwestern University (US)
- Corresponding author
- Brackett, Nicole F.
- Venue
- The CRISPR Journal
- Publisher
- SAGE Publications
- Published
- 2022-04-01
- Volume
- 5
- Issue
- 2
- Pages
- 213-223
- ISSN
- 2573-1599 (ISSN-L)
- 2573-1602
- OpenAlex
- W4220811559
- Cited by
- 31
- References
- 52
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 8 · 2023: 6 · 2022: 5
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The CRISPR Journal (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- Fel d 1, cat allergen, cat allergy, CRISPR/Cas9 genome editing, cats, Felidae, off-target analysis, asthma
- MeSH terms
- Gene Editing, Allergens, Animals, Biology, Cats, Glycoproteins, Hypersensitivity, CRISPR-Cas Systems
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