Molecular Characteristics and Immunomodulatory Activities of Water-Soluble Sulfated Polysaccharides from Ulva pertusa
Abstract
Sulfated polysaccharides isolated from Ulva pertusa and fractionated using anion-exchange chromatography were investigated to determine their molecular characteristics and bioactivities. The crude and fractionated polysaccharides (F(1), F(2), and F(3)) were mainly composed of carbohydrates (59.9-65.9%), sulfates (11.6-15.3%), and uronic acid (7.30-16.4%) with small amounts of proteins (1.40-4.80%). Rhamnose (62.5-80.7%) was the major monosaccharide unit of these polysaccharides, with different levels of glucose (13.5-27.4%) and xylose (2.74-11.5%). The polysaccharides contained one or two major subfractions with weight-average molecular mass ranging from 51.1×10(3) to 1,690×10(3) g/mol. The relatively low in vitro anticancer activity of the polysaccharides (22.3-42.4%) suggested that they had little cytotoxicity against the cancer cell line used (AGS). On the other hand, the polysaccharides significantly stimulated Raw 264.7 cells, inducing considerable amounts of nitric oxide and various cytokines production, which suggested that they could be strong immunostimulators.
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Ulvans from Ulva pertusa had little cytotoxicity against tumor cells.
“Ulvans from Ulva pertusa had little cytotoxicity against tumor cells, but significantly stimulated immunity, inducing considerable amounts of nitric oxide and cytokine production [111].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [111]
Ulvans from Ulva pertusa significantly stimulated immunity, inducing considerable amounts of nitric oxide and cytokine production.
“Ulvans from Ulva pertusa had little cytotoxicity against tumor cells, but significantly stimulated immunity, inducing considerable amounts of nitric oxide and cytokine production [111].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [111]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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- Authors
- Tabarsa, M., Han, J. H., Kim, C. Y., & You, S. G.
- Affiliations
- Gangneung–Wonju National University (KR)
- PepsiCo Advanced Research, Plano, Texas, USA.
- Gangneung Techno Valley, Gangneung, Gangwon-do, Korea.
- Corresponding author
- You, Sang Guan
- Venue
- Journal of Medicinal Food
- Publisher
- SAGE Publications
- Published
- 2012-02
- Volume
- 15
- Issue
- 2
- Pages
- 135-144
- ISSN
- 1096-620X (ISSN-L)
- 1557-7600
- OpenAlex
- W1998645387
- Cited by
- 113
- References
- 32
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 8 · 2023: 8 · 2022: 10 · 2021: 10 · 2020: 16 · 2019: 12 · 2018: 6 · 2017: 7 · 2016: 1 · 2015: 9 · 2014: 6 · 2013: 3 · 2012: 2
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medicinal Food (publishedVersion)
- PubMed (publishedVersion)
- The Royal Society of Chemistry’s Journals, Books and Databases (The Royal Society of Chemistry) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
- Keywords
- Ulva pertusa, sulfated polysaccharides, seaweed polysaccharides, uronic acid, rhamnose, polysaccharide monosaccharide composition, molecular weight distribution, anticancer activity, cytotoxicity, immunostimulation, nitric oxide production, cytokine production, RAW 264.7 macrophages, green macroalgae
- MeSH terms
- Animals, Humans, Immunologic Factors, Molecular Weight, Polysaccharides, Seaweed, Cytokines, Ulva, Cell Line, Tumor, Cell Proliferation, Mice
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