Immunomodulating activities of acidic sulphated polysaccharides obtained from the seaweed Ulva rigida C. Agardh
Abstract
Water-soluble acidic polysaccharides from the cell walls of Ulva rigida are mainly composed of disaccharides that contain glucuronic acid and sulphated rhamnose. The structure of disaccharides resembles that of glycosaminoglycans (GAGs) as they both contain glucuronic acid and sulphated sugars. Glycosaminoglycans occur in the extracellular matrix of animal connective tissues but can also be produced by leucocytes at inflammatory sites. Certain types of GAGs can even activate macrophages and therefore the acidic polysaccharides from U. rigida probably modulate macrophage activity. In the present study, we evaluated the effects of U. rigida polysaccharides on several RAW264.7 murine macrophage activities, including expression of inflammatory cytokines and receptors, nitric oxide and prostaglandin E2 (PGE(2)) production, and nitric oxide synthase 2 (NOS-2) and cyclooxygenase-2 (COX-2) gene expression. U. rigida acidic polysaccharides induced a more than two-fold increase in the expression of several chemokines (chemokine (C motif) ligand 1, chemokine (C-X-C motif) ligand 12, chemokine (C-C motif) ligand 22 and chemokine (C-X-C motif) ligand 14 (Cxcl14)) and in the expression of IL6 signal transducer and IL12 receptor beta 1. Incubation of macrophages with U. rigida polysaccharides also induced an increase in nitrite production, although this effect decreased considerably after desulphation of polysaccharides, suggesting that the sulphate group is important for the stimulatory capacity of these molecules. U. rigida polysaccharides also stimulated macrophage secretion of PGE(2) and induced an increase in COX-2 and NOS-2 expression. The results indicate that U. rigida acid polysaccharide can be used as an experimental immunostimulant for analysing inflammatory responses related to macrophage functions. In addition, these polysaccharides may also be of clinical interest for modifying certain macrophage activities in diseases where macrophage function is impaired or needs to be boosted.
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Ulvan from Ulva rigida induced more than a two times increase in the expression of some cytokines.
“The ulvan from Ulva rigida induced more than a two times increase in the expression of some cytokines, stimulated the secretion and activity of murine macophages as well as inducing an increase in COX-2 and NOS-2 expression [110].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [110]
Ulvan from Ulva rigida induced an increase in COX-2 and NOS-2 expression.
“The ulvan from Ulva rigida induced more than a two times increase in the expression of some cytokines, stimulated the secretion and activity of murine macophages as well as inducing an increase in COX-2 and NOS-2 expression [110].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [110]
Ulvan from Ulva rigida stimulated the secretion and activity of murine macrophages.
“The ulvan from Ulva rigida induced more than a two times increase in the expression of some cytokines, stimulated the secretion and activity of murine macophages as well as inducing an increase in COX-2 and NOS-2 expression [110].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [110]
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- Authors
- Leiro, J. M., Castro, R., Arranz, J. A., & Lamas, J.
- Affiliations
- Universidade de Santiago de Compostela (ES)
- Corresponding author
- Lamas, Jesús
- Venue
- International Immunopharmacology
- Publisher
- Elsevier BV
- Published
- 2007-07
- Volume
- 7
- Issue
- 7
- Pages
- 879-888
- ISSN
- 1567-5769 (ISSN-L)
- OpenAlex
- W2044335811
- Cited by
- 316
- References
- 52
- Citations by year
- 2026: 4 · 2025: 12 · 2024: 20 · 2023: 15 · 2022: 20 · 2021: 26 · 2020: 31 · 2019: 18 · 2018: 22 · 2017: 24 · 2016: 18 · 2015: 24 · 2014: 18 · 2013: 12 · 2012: 17
- FWCI
- 10.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Immunopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Keywords
- Ulva rigida, seaweed polysaccharides, sulfated polysaccharides, glycosaminoglycans, macrophage activation, RAW 264.7 macrophages, inflammatory cytokines, chemokines, nitric oxide, prostaglandin E2, cyclooxygenase-2, NOS2, sulfate groups, immunomodulation, desulphation
- MeSH terms
- Animals, Cell Line, Gene Expression Regulation, Immunologic Factors, Inflammation, Macrophage Activation, Macrophages, Nitric Oxide, Polysaccharides, Sulfuric Acids, Dinoprostone, Chemokines, Ulva, Mice, Cyclooxygenase 2, Nitric Oxide Synthase Type II
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