Cited by 30Parasitic Diseases Research and TreatmentHelminth infection and controlParasites and Host Interactions

Anti-Wolbachia Surface Protein Antibodies Are Present in the Urine of Dogs Naturally Infected with Dirofilaria immitis with Circulating Microfilariae But Not in Dogs with Occult Infections

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Morchón, Rodrigo, Carretón, Elena, Grandi, Giulio, González-Miguel, Javier, Montoya-Alonso, J. Alberto, Simón, Fernando, Genchi, Claudio, Kramer, Laura H.·2012·Vector-Borne and Zoonotic Diseases

Abstract

Heartworm infection (Dirofilaria immitis) can cause kidney damage due to the presence of circulating microfilariae (mf) that contribute to the production and deposit of immune complexes. It has been shown that mf are a major source of Wolbachia antigen during active infection. Here the authors compared urine samples from 19 naturally infected dogs with (mf+) and 12 without (mf-) microfilariae for the presence of proteinuria and anti-Wolbachia Surface Protein (-WSP) IgG in ELISA. Kidneys from 6 mf+ and 3 mf- dogs were also examined by anti-WSP immuno-histochemistry. All infected dogs showed proteinuria, but mf+ dogs had significantly higher values compared to mf-dogs. Mf+ dogs had optical density values for anti-WSP IgG consistently higher than established cut-off values and were significantly higher than values for mf- dogs. Kidneys from mf+ dogs showed Wolbachia+ mf in glomerular capillaries. Results strongly suggest that Wolbachia associated with circulating mf may contribute to immune-mediated kidney disease in dogs with heartworm infection.

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Crossref · 10.1089/vbz.2010.0211

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  • Among dogs with glomerulonephritis and proteinuria linked to dirofilariasis, anti-WSP IgG levels in urine are higher in microfilaremic than amicrofilaremic animals.

    “Among dogs with glomerulonephritis and proteinuria linked to dirofilariasis, higher levels of anti-WSP IgG antibodies were detected in the urine of microfilaremic animals than in amicrofilaremic animals (288).”
  • Wolbachia is present in multiple tissues and immune cells of dogs with heartworm disease, as shown by immunohistochemical staining.

    “immunohistochemical staining with polyclonal antibodies against WSP has revealed the presence of Wolbachia in multiple tissues and immune cells of dogs with heartworm disease (215,216,288).”

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Authors
Morchón, R., Carretón, E., Grandi, G., González-Miguel, J., Montoya-Alonso, J. A., Simón, F., Genchi, C., & Kramer, L. H.
Affiliations
  • University of Parma (IT)
  • Universidad de Las Palmas de Gran Canaria (ES)
  • Laboratory of Parasitology, Faculty of Pharmacy, University of Salamanca, Salamanca, Spain.
  • Universidad de Salamanca (ES)
  • University of Milan (IT)
Corresponding author
Kramer, Laura H.
Venue
Vector-Borne and Zoonotic Diseases
Published
2012-01
Volume
12
Issue
1
Pages
17-20
ISSN
  • 1530-3667 (ISSN-L)
  • 1557-7759
OpenAlex
W2041208399
Cited by
30
References
17
Citations by year
2025: 4 · 2024: 4 · 2023: 4 · 2022: 4 · 2020: 6 · 2019: 1 · 2017: 1 · 2015: 1 · 2014: 3 · 2012: 2
FWCI
1.08
Type
journal article
Language
en
Open access
Locations
  • Vector-Borne and Zoonotic Diseases (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Acceda (Universidad de Las Palmas de Gran Canaria) (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
Keywords
Dirofilaria immitis, canine heartworm disease, Wolbachia, occult infection, proteinuria, kidney injury, immune complexes, glomerular deposition
MeSH terms
Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Dirofilaria immitis, Dirofilariasis, Dog Diseases, Dogs, Microfilariae, Wolbachia

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