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Interorganellar Regulation of Lysosome Positioning by the Golgi Apparatus through Rab34 Interaction with Rab-interacting Lysosomal Protein
Open accessAbstract
We present evidence to suggest the existence of a regulatory pathway for the Golgi apparatus to modulate the spatial positioning of otherwise distantly located lysosomes. Rab34, a new member of the Rab GTPase family, is associated primarily with the Golgi apparatus. Expression of wild-type or GTP-restricted but not GDP-restricted versions of Rab34 causes spatial redistribution of lysosomes from the periphery to the peri-Golgi region. The regulation of lysosomal positioning by Rab34 depends on its association with the membrane mediated by prenylation and its direct interaction with Rab-interacting lysosomal protein (RILP). This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region. Our results have uncovered a novel mechanism for the Golgi apparatus to regulate the spatial distribution of another organelle.
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“We found a very high location similarity between lysosomes and Golgi, consistent with their enrichment in location in the apical cytoplasm and the known role of the Golgi in regulating lysosome localization20,21.”
From Integrated intracellular organization and its variations in human iPS cells (2023) · cites this paper as [20]
In the citing paper
“overexpression of Rab34, Rab36, Rabring7, or Rapsyn causes clustering of lysosomes in the perinuclear area.”
From TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes (2017) · cites this paper as [31]
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Metadata
- Authors
- Wang, T., & Hong, W.
- Affiliations
- Institute of Molecular and Cell Biology (SG)
- Venue
- Molecular Biology of the Cell
- Published
- 2002-12
- Volume
- 13
- Issue
- 12
- Pages
- 4317-4332
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC138636
- OpenAlex
- W2137315893
- Cited by
- 147
- References
- 48
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 11 · 2023: 6 · 2022: 8 · 2021: 9 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 13 · 2016: 6 · 2015: 5 · 2014: 8 · 2013: 3 · 2012: 6
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National University of Singapore
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- lysosome positioning, Golgi apparatus, Rab34, Rab GTPases, RILP, prenylation, switch I region
- MeSH terms
- Amino Acid Sequence, Animals, Axons, Base Sequence, Carrier Proteins, Cells, Cultured, Cloning, Molecular, Cytoskeleton, Glutathione Transferase, Golgi Apparatus, HeLa Cells, Humans, Lysine, Lysosomes, Microscopy, Fluorescence, Models, Genetic, Molecular Sequence Data, Plant Proteins, Protein Binding, Transfection, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, DNA, Complementary, Fluorescent Antibody Technique, Indirect, rab GTP-Binding Proteins, Two-Hybrid System Techniques, Adaptor Proteins, Signal Transducing, Mice, Rats
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