Cited by 107Proteoglycans and glycosaminoglycans researchNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

Versican V1 Isoform Induces Neuronal Differentiation and Promotes Neurite Outgrowth

Open access
Wu, Yaojiong, Sheng, Wang, Chen, Liwen, Dong, Haiheng, Lee, Vivian, Lu, Fred, Wong, C. Shun, Lu, Wei-Yang +1 more·2004·Molecular Biology of the Cell

Abstract

The chondroitin sulfate proteoglycan versican is one of the major extracellular components in the developing and adult brain. Here, we show that isoforms of versican play different roles in neuronal differentiation and neurite outgrowth. Expression of versican V1 isoform in PC12 cells induced complete differentiation, whereas expression of V2 induced an aborted differentiation accompanied by apoptosis. V1 promoted neurite outgrowth of hippocampal neurons, but V2 failed to do so. V1 transfection enhanced expression of epidermal growth factor receptor and integrins, and facilitated sustained extracellular signal-regulated kinase/MAPK phosphorylation. Blockade of the epidermal growth factor receptor, β1 integrin, or Src significantly inhibited neuronal differentiation. Finally, we demonstrated that versican V1 isoform also promoted differentiation of neural stem cells into neurons. Our results have implications for understanding how versican regulates neuronal development, function, and repair.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.e03-09-0667

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 50 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Wu, Y., Sheng, W., Chen, L., Dong, H., Lee, V., Lu, F., Wong, C. S., Lu, W.-Y., & Yang, B. B.
Affiliations
  • Sunnybrook Health Science Centre (CA)
  • Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario M4N 3M5, Canada
Venue
Molecular Biology of the Cell
Published
2004-05
Volume
15
Issue
5
Pages
2093-2104
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W2146306557
Cited by
107
References
50
Citations by year
2025: 1 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 9 · 2020: 5 · 2019: 4 · 2018: 8 · 2017: 3 · 2016: 3 · 2015: 7 · 2014: 7 · 2013: 2 · 2012: 6
FWCI
3.30
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Heart and Stroke Foundation of Canada
  • Canadian Institutes of Health Research
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
neuronal differentiation, neurite outgrowth, chondroitin sulfate proteoglycans, PC12 cells, hippocampal neurons, epidermal growth factor receptor, beta1 integrin, Src kinase, ERK signaling, neural stem cell differentiation, apoptosis, neuronal development, brain extracellular matrix
MeSH terms
Animals, Carrier Proteins, Cell Differentiation, Genetic Vectors, Glycoproteins, Hippocampus, Nerve Growth Factors, Nerve Tissue Proteins, Neurons, Phosphorylation, Quinones, Transfection, Gene Expression, Integrins, Cyclins, Benzoquinones, Neurites, PC12 Cells, Rifabutin, Integrin beta1, Protein Isoforms, Mitogen-Activated Protein Kinase Kinases, Lactams, Macrocyclic, Rats, Versicans, ErbB Receptors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review