Brain derived versican V2 is a potent inhibitor of axonal growth
Abstract
In this paper, we identify the chondroitin sulfate proteoglycan versican V2 as a major inhibitor of axonal growth in the extracellular matrix of the mature central nervous system. In immunohistochemical and in situ hybridization experiments we show that this tissue-specific splice variant of versican is predominantly present in myelinated fiber tracts of the brain and in the optic nerve, most likely being expressed by oligodendrocytes. We demonstrate that isolated versican V2 strongly inhibits neurite outgrowth of central and peripheral neurons in stripe-choice assays using laminin-1 as permissive substrate. The inhibitory character of versican V2 is maintained after removal of chondroitin sulfate and N- and O-linked oligosaccharide side chains, but it is abolished after core protein digestion with proteinase-K. Our data support the notion, that intact versican V2 prevents excessive axonal growth during late phases of development and hereby participates in the structural stabilization of the mature central nervous system.
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Versican V2 and Hapln2/Bral1 are most ubiquitously located at the nodes.
“Versican V2 and Hapln2/Bral1 are most ubiquitously located at the nodes (Schmalfeldt et al., 2000;Oohashi et al., 2002).”
From The hyaluronan and proteoglycan link proteins: Organizers of the brain extracellular matrix and key molecules for neuronal function and plasticity (2015) · cites this paper as Schmalfeldt et al., 2000
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- Authors
- Schmalfeldt, M., Bandtlow, C. E., Dours-Zimmermann, M. T., Winterhalter, K. H., & Zimmermann, D. R.
- Affiliations
- University of Zurich (CH)
- University of Zurich, 8091 Zürich 1 Molecular Biology Laboratory, Department of Pathology , , Switzerland
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2000-03-01
- Volume
- 113
- Issue
- 5
- Pages
- 807-816
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2118008848
- Cited by
- 197
- References
- 62
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 9 · 2015: 7 · 2014: 10 · 2013: 4 · 2012: 9
- FWCI
- 4.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Keywords
- axon growth inhibition, neurite outgrowth, chondroitin sulfate proteoglycans, extracellular matrix, oligodendrocytes, optic nerve, central nervous system maturation, laminin-1, proteoglycan core protein
- MeSH terms
- Animals, Axons, Brain, Cattle, Growth Inhibitors, Neural Inhibition, Chondroitin Sulfate Proteoglycans, Neurites, Lectins, C-Type, Mice, Versicans
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