The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
The Sequential Activation of the Yeast HOG and SLT2 Pathways Is Required for Cell Survival to Cell Wall Stress
Open accessAbstract
Yeast mitogen-activated protein kinase (MAPK) signaling pathways transduce external stimuli into cellular responses very precisely. The MAPKs Slt2/Mpk1 and Hog1 regulate transcriptional responses of adaptation to cell wall and osmotic stresses, respectively. Unexpectedly, we observe that the activation of a cell wall integrity (CWI) response to the cell wall damage caused by zymolyase (β-1,3 glucanase) requires both the HOG and SLT2 pathways. Zymolyase activates both MAPKs and Slt2 activation depends on the Sho1 branch of the HOG pathway under these conditions. Moreover, adaptation to zymolyase requires essential components of the CWI pathway, namely the redundant MAPKKs Mkk1/Mkk2, the MAPKKK Bck1, and Pkc1, but it does not require upstream elements, including the sensors and the guanine nucleotide exchange factors of this pathway. In addition, the transcriptional activation of genes involved in adaptation to cell wall stress, like CRH1, depends on the transcriptional factor Rlm1 regulated by Slt2, but not on the transcription factors regulated by Hog1. Consistent with these findings, both MAPK pathways are essential for cell survival under these circumstances because mutant strains deficient in different components of both pathways are hypersensitive to zymolyase. Thus, a sequential activation of two MAPK pathways is required for cellular adaptation to cell wall damage.
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“Protein kinase C (PKC), high osmolarity glycerol response (HOG), and Ca+2-calcineurin pathways have been implicated in the response to cell wall damage and chitin synthesis (Figure 1B; Lagorce et al., 2003; Bermejo et al., 2008; Walker et al., 2008; Fortwendel et al., 2009).”
From Fungal Cell Wall: Emerging Antifungals and Drug Resistance. (2019) · cites this paper as Bermejo et al., 2008
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Cell wall remodeling is required for crowding homeostasis during cell growth in S. cerevisiaePreprint
Sakai, K., Kunzi, M., Uliana, F., Willig, L., Lappalainen, R., Kamada, Y., Neurohr, G. E., & Ikui, A. E. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.06.12.731398 · Cited by 1
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García, R., Mascaraque, V., Vozmediano-Peraita, L., Sanz, A. B., Díez-Muñiz, S., Rodríguez-Peña, J. M., & Arroyo, J. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.7506230
Self-citationEngineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular LifespanPreprint
Oz, N., Su, H., Sarı, V., Patnaik, P. K., Hameed, R., Song, J. H., Prosser, D. C., Labunskyy, V. M., Gladyshev, V. N., Hao, N., et al. (2026) · eLife · doi:10.7554/elife.109761.2
Metadata
- Authors
- Bermejo, C., Rodríguez, E., García, R., Rodríguez-Peña, J. M., Rodríguez de la Concepción, M. L., Rivas, C., Arias, P., Nombela, C., Posas, F., & Arroyo, J.
- Affiliations
- Universidad Complutense de Madrid (ES)
- *Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; and
- Cell Signalling Unit, Department de Ciènces Experimentals I de la Salut, Universitat Pompeu Fabra (UPF), Parc de Recerca Biomèdica de Barcelona (PRBB), E-08003 Barcelona, Spain
- Universitat Pompeu Fabra (ES)
- ORCID
- Venue
- Molecular Biology of the Cell
- Published
- 2008-03
- Volume
- 19
- Issue
- 3
- Pages
- 1113-1124
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC2262984
- OpenAlex
- W2103491947
- Cited by
- 204
- References
- 60
- Citations by year
- 2026: 11 · 2025: 4 · 2024: 8 · 2023: 6 · 2022: 9 · 2021: 7 · 2020: 8 · 2019: 9 · 2018: 9 · 2017: 6 · 2016: 16 · 2015: 18 · 2014: 17 · 2013: 7 · 2012: 15
- FWCI
- 7.41
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular Biology of the Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Johns Hopkins University
- Ministerio de Economía y Competitividad
- Universitat de Lleida
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- HOG pathway, cell wall integrity, MAPK signaling, zymolyase, β-1,3-glucanase, cell wall stress, osmotic stress, Sho1, PKC1, Rlm1, Saccharomyces cerevisiae, stress adaptation, cell survival
- MeSH terms
- Adaptation, Physiological, Cell Wall, Enzyme Activation, Hydrolases, Mutation, Phosphorylation, Saccharomyces cerevisiae, Transcriptional Activation, Mitogen-Activated Protein Kinases, MAP Kinase Signaling System, Saccharomyces cerevisiae Proteins, Microbial Viability
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