The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis
Open accessAbstract
Individual metastatic tumor cells exhibit two interconvertible modes of cell motility during tissue invasion that are classified as either mesenchymal or amoeboid. The molecular mechanisms by which invasive breast cancer cells regulate this migratory plasticity have yet to be fully elucidated. Herein we show that the focal adhesion adaptor protein, paxillin, and the closely related Hic-5 have distinct and unique roles in the regulation of breast cancer cell lung metastasis by modulating cell morphology and cell invasion through three-dimensional extracellular matrices (3D ECMs). Cells depleted of paxillin by RNA interference displayed a highly elongated mesenchymal morphology, whereas Hic-5 knockdown induced an amoeboid phenotype with both cell populations exhibiting reduced plasticity, migration persistence, and velocity through 3D ECM environments. In evaluating associated signaling pathways, we determined that Rac1 activity was increased in cells devoid of paxillin whereas Hic-5 silencing resulted in elevated RhoA activity and associated Rho kinase–induced nonmuscle myosin II activity. Hic-5 was essential for adhesion formation in 3D ECMs, and analysis of adhesion dynamics and lifetime identified paxillin as a key regulator of 3D adhesion assembly, stabilization, and disassembly.
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Cites
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Genes that mediate breast cancer metastasis to lung
Minn, A. J., Gupta, G. P., Siegel, P. M., Bos, P. D., Shu, W., Giri, D. D., Viale, A., Olshen, A. B., Gerald, W. L., & Massagué, J. (2005) · Nature · doi:10.1038/nature03799 · Cited by 2,922
Rapid leukocyte migration by integrin-independent flowing and squeezing
Lämmermann, T., Bader, B. L., Monkley, S. J., Worbs, T., Wedlich-Söldner, R., Hirsch, K., Keller, M., Förster, R., Critchley, D. R., Fässler, R., et al. (2008) · Nature · doi:10.1038/nature06887 · Cited by 1,397
Strategies for MMP inhibition in cancer: innovations for the post-trial era
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Long-term human breast carcinoma cell lines of metastatic origin: Preliminary characterization
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PDK1 Regulates Cancer Cell Motility by Antagonising Inhibition of ROCK1 by RhoE
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Di Vizio, D., Morello, M., Dudley, A. C., Schow, P. W., Adam, R. M., Morley, S., Mulholland, D., Rotinen, M., Hager, M. H., Insabato, L., et al. (2012) · The American Journal of Pathology · doi:10.1016/j.ajpath.2012.07.030 · Cited by 422
Nonpolarized signaling reveals two distinct modes of 3D cell migration
Petrie, R. J., Gavara, N., Chadwick, R. S., & Yamada, K. M. (2012) · Journal of Cell Biology · doi:10.1083/jcb.201201124 · Cited by 375
At the leading edge of three-dimensional cell migration
Petrie, R. J., & Yamada, K. M. (2012) · Journal of Cell Science · doi:10.1242/jcs.093732 · Cited by 317
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Metadata
- Authors
- Deakin, N. O., & Turner, C. E.
- Venue
- Molecular Biology of the Cell
- Published
- 2011-02
- Volume
- 22
- Issue
- 3
- Pages
- 327-341
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2006187225
- Cited by
- 165
- References
- 7
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- green
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- Deakin, N. O., & Turner, C. E. (2011). Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e10-09-0790
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