The DOI matches its Crossref record; 91 of the 92 references it cites were found in a registry or an index.
Identification of cell cycle-regulated genes periodically expressed in U2OS cells and their regulation by FOXM1 and E2F transcription factors
Open accessAbstract
We identify the cell cycle-regulated mRNA transcripts genome-wide in the osteosarcoma-derived U2OS cell line. This results in 2140 transcripts mapping to 1871 unique cell cycle-regulated genes that show periodic oscillations across multiple synchronous cell cycles. We identify genomic loci bound by the G2/M transcription factor FOXM1 by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) and associate these with cell cycle-regulated genes. FOXM1 is bound to cell cycle-regulated genes with peak expression in both S phase and G2/M phases. We show that ChIP-seq genomic loci are responsive to FOXM1 using a real-time luciferase assay in live cells, showing that FOXM1 strongly activates promoters of G2/M phase genes and weakly activates those induced in S phase. Analysis of ChIP-seq data from a panel of cell cycle transcription factors (E2F1, E2F4, E2F6, and GABPA) from the Encyclopedia of DNA Elements and ChIP-seq data for the DREAM complex finds that a set of core cell cycle genes regulated in both U2OS and HeLa cells are bound by multiple cell cycle transcription factors. These data identify the cell cycle-regulated genes in a second cancer-derived cell line and provide a comprehensive picture of the transcriptional regulatory systems controlling periodic gene expression in the human cell division cycle.
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“We compared these machine-reported gene lists with four published datasets for experimentally characterized cell cycle genes (including the HeLa data we used as seeds) (Grant et al., 2013)”
From Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing (2019) · cites this paper as Grant et al., 2013
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Metadata
- Authors
- Grant, G. D., Brooks, L., Zhang, X., Mahoney, J. M., Martyanov, V., Wood, T. A., Sherlock, G., Cheng, C., & Whitfield, M. L.
- Affiliations
- Dartmouth College (US)
- Stanford Medicine (US)
- Corresponding author
- Whitfield, Michael L.
- ORCID
- Venue
- Molecular Biology of the Cell
- Published
- 2013-12
- Volume
- 24
- Issue
- 23
- Pages
- 3634-3650
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC3842991
- OpenAlex
- W2102123859
- Cited by
- 179
- References
- 92
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 13 · 2023: 6 · 2022: 10 · 2021: 21 · 2020: 19 · 2019: 17 · 2018: 14 · 2017: 20 · 2016: 17 · 2015: 14 · 2014: 12
- FWCI
- 6.42
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- Foundation for the National Institutes of Health
- V Foundation for Cancer Research
- National Human Genome Research Institute (R01HG004499)
- National Cancer Institute (R25CA134286, R01CA130795)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- cell cycle-regulated genes, periodic gene expression, FOXM1, E2F transcription factors, G2/M phase, S phase, osteosarcoma, U2OS cells, transcriptional regulation, DREAM complex, GABPA, E2F1, E2F4, E2F6, promoter activation
- MeSH terms
- Forkhead Box Protein M1, Cell Cycle, Multigene Family, HeLa Cells, Humans, Protein Binding, RNA, Messenger, Time Factors, Transcription, Genetic, Protein Biosynthesis, Gene Expression Regulation, Neoplastic, Genes, cdc, Gene Expression Profiling, Chromatin Immunoprecipitation, E2F Transcription Factors, Forkhead Transcription Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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