Cited by 26Lipid metabolism and biosynthesisGenetics, Aging, and Longevity in Model OrganismsAutophagy in Disease and Therapy

The transfer of specific mitochondrial lipids and proteins to lipid droplets contributes to proteostasis upon stress and aging in the eukaryotic model system Saccharomyces cerevisiae

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Geltinger, Florian, Tevini, Julia, Briza, Peter, Geiser, Amrito, Bischof, Johannes, Richter, Klaus, Felder, Thomas, Rinnerthaler, Mark·2020·GeroScience

Abstract

Originally Lipid droplets (LDs) were considered as being droplets for lipid storage only. Increasing evidence, however, demonstrates that LDs fulfill a pleiotropy of additional functions. Among them is the modulation of protein as well as lipid homeostasis. Under unfavorable pro-oxidative conditions, proteins can form aggregates which may exceed the overall proteolytic capacity of the proteasome. After stress termination LDs can adjust and support the removal of these aggregates. Additionally, LDs interact with mitochondria, specifically take over certain proteins and thus prevent apoptosis. LDs, which are loaded with these harmful proteins, are subsequently eliminated via lipophagy. Recently it was demonstrated that this autophagic process is a modulator of longevity. LDs do not only eliminate potentially dangerous proteins, but they are also able to prevent lipotoxicity by storing specific lipids. In the present study we used the model organism Saccharomyces cerevisiae to compare the proteome as well as lipidome of mitochondria and LDs under different conditions: replicative aging, stress and apoptosis. In this context we found an accumulation of proteins at LDs, supporting the role of LDs in proteostasis. Additionally, the composition of main lipid classes such as phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylglycerols, triacylglycerols, ceramides, phosphatidic acids and ergosterol of LDs and mitochondria changed during stress conditions and aging.

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Crossref · 10.1007/s11357-019-00103-0

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  • PG is a mitochondrial PL that is increased in stressed or aged microglia and is converted to cardiolipin.

    “Phosphatidylglycerol (PG) is a mitochondrial PL that is increased in stressed or aged microglia and is converted to cardiolipin (CL) [42].”

    From Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation (2021) · cites this paper as [42]

    In Results and discussion, under “Stress and aging change the lipidome of LDs and mitochondria” (page not supplied), the paper reports increased mitochondrial PG in mildly stressed and replicatively aged yeast cells and describes PG as a precursor of cardiolipin. It does not study microglia, so the claimed increase in stressed or aged microglia is not supported by this source. (LLM, conf=0.91)

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Research works printed but not linked: 1

  1. Rodrigues ML (2018) Overexpression or Deletion of Ergosterol Biosynthesis Genes Alters Doubling Time, Response to Stress Agents, and Drug Susceptibility inSaccharomyces cerevisiae. The Multifunctional Fungal Ergosterol Mbio 9. 10.1128/mBio.01291-18 PMC6058291 30042199 · DOI · Paper page

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Authors
Geltinger, F., Tevini, J., Briza, P., Geiser, A., Bischof, J., Richter, K., Felder, T., & Rinnerthaler, M.
Affiliations
  • University of Salzburg (AT)
  • Paracelsus Medical University (AT)
Corresponding author
  • Felder, Thomas
  • Rinnerthaler, Mark
Venue
GeroScience
Published
2019-11-01
Volume
42
Issue
1
Pages
19-38
ISSN
  • 2152-4041 (ISSN-L)
  • 2509-2715
  • 2509-2723
OpenAlex
W2988566186
Cited by
26
References
90
Citations by year
2026: 3 · 2025: 2 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 5 · 2020: 1
FWCI
1.15
Type
journal article
Language
en
Open access
Locations
  • GeroScience (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
PMU (PMU-FFF E-16/23/117-FEA)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
lipid droplets, mitochondria, proteostasis, aging, stress response, apoptosis, lipophagy, protein aggregation, lipotoxicity, lipidome, mitochondrial lipid composition, Saccharomyces cerevisiae, replicative senescence
MeSH terms
Proteostasis, Lipids, Mitochondria, Saccharomyces cerevisiae, Lipid Metabolism, Lipid Droplets

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