The transfer of specific mitochondrial lipids and proteins to lipid droplets contributes to proteostasis upon stress and aging in the eukaryotic model system Saccharomyces cerevisiae
Open accessAbstract
Originally Lipid droplets (LDs) were considered as being droplets for lipid storage only. Increasing evidence, however, demonstrates that LDs fulfill a pleiotropy of additional functions. Among them is the modulation of protein as well as lipid homeostasis. Under unfavorable pro-oxidative conditions, proteins can form aggregates which may exceed the overall proteolytic capacity of the proteasome. After stress termination LDs can adjust and support the removal of these aggregates. Additionally, LDs interact with mitochondria, specifically take over certain proteins and thus prevent apoptosis. LDs, which are loaded with these harmful proteins, are subsequently eliminated via lipophagy. Recently it was demonstrated that this autophagic process is a modulator of longevity. LDs do not only eliminate potentially dangerous proteins, but they are also able to prevent lipotoxicity by storing specific lipids. In the present study we used the model organism Saccharomyces cerevisiae to compare the proteome as well as lipidome of mitochondria and LDs under different conditions: replicative aging, stress and apoptosis. In this context we found an accumulation of proteins at LDs, supporting the role of LDs in proteostasis. Additionally, the composition of main lipid classes such as phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylglycerols, triacylglycerols, ceramides, phosphatidic acids and ergosterol of LDs and mitochondria changed during stress conditions and aging.
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PG is a mitochondrial PL that is increased in stressed or aged microglia and is converted to cardiolipin.
“Phosphatidylglycerol (PG) is a mitochondrial PL that is increased in stressed or aged microglia and is converted to cardiolipin (CL) [42].”
From Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation (2021) · cites this paper as [42]
In Results and discussion, under “Stress and aging change the lipidome of LDs and mitochondria” (page not supplied), the paper reports increased mitochondrial PG in mildly stressed and replicatively aged yeast cells and describes PG as a precursor of cardiolipin. It does not study microglia, so the claimed increase in stressed or aged microglia is not supported by this source. (LLM, conf=0.91)
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Bohnert, M. (2018) · The Journal of Cell Biology · doi:10.1083/jcb.201811021 · Cited by 11 · see citing works
Behead and live long or the tale of cathepsin L
Streubel, M. K., Bischof, J., Weiss, R., Duschl, J., Liedl, W., Wimmer, H., Breitenbach, M., Weber, M., Geltinger, F., Richter, K., et al. (2017) · Yeast · doi:10.1002/yea.3286 · Cited by 7 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 1
- Rodrigues ML (2018) Overexpression or Deletion of Ergosterol Biosynthesis Genes Alters Doubling Time, Response to Stress Agents, and Drug Susceptibility inSaccharomyces cerevisiae. The Multifunctional Fungal Ergosterol Mbio 9. 10.1128/mBio.01291-18 PMC6058291 30042199 · DOI · Paper page
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Sorted by citations
Papsdorf, K., Miklas, J. W., Hosseini, A., Cabruja, M., Morrow, C. S., Savini, M., Yu, Y., Silva-García, C. G., Haseley, N. R., Murphy, L., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01136-6 · Cited by 145 · see citing works
Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation
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“Phosphatidylglycerol (PG) is a mitochondrial PL that is increased in stressed or aged microglia and is converted to cardiolipin (CL) [42].”
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Self-citationFunctional interplay of lipid droplets and mitochondria
Enkler, L., & Spang, A. (2024) · FEBS Letters · doi:10.1002/1873-3468.14809 · Cited by 50 · see citing works
The Janus-Faced Role of Lipid Droplets in Aging: Insights from the Cellular Perspective
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Self-citationKrawczyk, H. E., Sun, S., Doner, N. M., Yan, Q., Lim, M. S. S., Scholz, P., Niemeyer, P. W., Schmitt, K., Valerius, O., Pleskot, R., et al. (2022) · The Plant Cell · doi:10.1093/plcell/koac095 · Cited by 27 · see citing works
Circulating Mitochondrial DNA and Inter-Organelle Contact Sites in Aging and Associated Conditions
Picca, A., Guerra, F., Calvani, R., Romanò, R., Coelho‐Júnior, H. J., Damiano, F. P., Bucci, C., & Marzetti, E. (2022) · Cells · doi:10.3390/cells11040675 · Cited by 26 · see citing works
Lipids and the hallmarks of ageing: From pathology to interventions
Sharma, R., & Diwan, B. (2023) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2023.111858 · Cited by 26 · see citing works
Eigenfeld, M., Kerpes, R., & Becker, T. M. (2021) · Frontiers in Fungal Biology · doi:10.3389/ffunb.2021.665490 · Cited by 24 · see citing works
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Valencia‐Olvera, A. C., Balu, D., Faulk, N., Amiridis, A., Wang, Y., Pham, C., Avila-Munoz, E., York, J. M., Thatcher, G. R. J., & LaDu, M. J. (2023) · Neurotherapeutics · doi:10.1007/s13311-023-01375-3 · Cited by 22 · see citing works
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Kovacs, M., Geltinger, F., Verwanger, T., Weiss, R., Richter, K., & Rinnerthaler, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.774985 · Cited by 21 · see citing works
Self-citationChiariello, A., Rossetti, L. M., Valente, S., Pasquinelli, G., Sollazzo, M., Iommarini, L., Porcelli, A. M., Tognocchi, M., Conte, G., Santoro, A., et al. (2024) · Aging Cell · doi:10.1111/acel.14111 · Cited by 17 · see citing works
Schürmanns, L., Hamann, A., & Osiewacz, H. D. (2022) · Cells · doi:10.3390/cells11030519 · Cited by 10 · see citing works
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Felder, T. K., Geltinger, F., & Rinnerthaler, M. (2021) · Aging · doi:10.18632/aging.202883 · Cited by 6 · see citing works
Self-citationKovacs, M., Geltinger, F., Schartel, L., Pöschl, S., Briza, P., Paschinger, M., Boros, K., Felder, T. K., Wimmer, H., Duschl, J., et al. (2023) · Journal of Lipid Research · doi:10.1016/j.jlr.2023.100473 · Cited by 5 · see citing works
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Characterization of Tgl2, a putative lipase in yeast mitochondriaPreprint
Tiku, V., Tatsuta, T., Jung, M., Rapaport, D., & Dimmer, K. S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.05.08.593122 · Cited by 1 · see citing works
SLDP and LIPA mediate lipid droplet-plasma membrane tethering in Arabidopsis thalianaPreprint
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Deolal, P., Ramalingam, K., Das, B., & Mishra, K. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.03.31.535026
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Lipid droplets at the interface of plant defense and pathogen exploitation
Arvy, N., Batsale, M., Shakir, S., Jambou, L., Bréhélin, C., & German‐Retana, S. (2026) · Frontiers in Plant Science · doi:10.3389/fpls.2026.1911736
- Authors
- Geltinger, F., Tevini, J., Briza, P., Geiser, A., Bischof, J., Richter, K., Felder, T., & Rinnerthaler, M.
- Affiliations
- University of Salzburg (AT)
- Paracelsus Medical University (AT)
- Corresponding author
- Felder, Thomas
- Rinnerthaler, Mark
- Venue
- GeroScience
- Published
- 2019-11-01
- Volume
- 42
- Issue
- 1
- Pages
- 19-38
- ISSN
- 2152-4041 (ISSN-L)
- 2509-2715
- 2509-2723
- PMC
- PMC7031196
- OpenAlex
- W2988566186
- Cited by
- 26
- References
- 90
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 5 · 2020: 1
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- GeroScience (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- PMU (PMU-FFF E-16/23/117-FEA)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- lipid droplets, mitochondria, proteostasis, aging, stress response, apoptosis, lipophagy, protein aggregation, lipotoxicity, lipidome, mitochondrial lipid composition, Saccharomyces cerevisiae, replicative senescence
- MeSH terms
- Proteostasis, Lipids, Mitochondria, Saccharomyces cerevisiae, Lipid Metabolism, Lipid Droplets
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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