Triacylglycerol-rich lipoprotein margination: a potential surrogate for whole-body lipoprotein lipase activity and effects of eicosapentaenoic and docosahexaenoic acids
Open accessAbstract
Background Margination occurs when blood borne particles attach to the vessel wall. Triacylglycerol-rich lipoprotein (TRL) particles marginate when they bind to endothelial lipoprotein lipase (LpL).Objective This study was undertaken to determine whether TRL margination reflects in vivo LpL activity and whether n-3 fatty acids affect fasting and fed TRL margination.Design Healthy subjects (n = 33) began with a 4-wk, placebo (olive oil; 4 g/d) run-in period and were then randomly assigned to 4 wk of treatment with 4 g/d of ethyl esters of either safflower oil (SAF; control), eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA). Margination volume (MV) was calculated by subtracting true from apparent plasma volume.Results MVs were 3 times as great during the fasting state as during the fed state (P < 0.0001). In both the fasting and the fed states, MV was significantly correlated with plasma triacylglycerol and TRL half-lives. In the fed state, MV was also correlated with preheparin LpL, whereas in the fasting state it was not. There was no significant correlation between preheparin LpL and postheparin LpL in the fasting state. Relative to SAF, EPA and DHA supplementation resulted in higher MVs by 64% and 53% (both P < 0.001), respectively, in the fasting state, without significantly reducing fasting triacylglycerol concentrations. In the fed state, DHA doubled the MV (P < 0.05), whereas EPA had no significant effect.Conclusions The correlations between MV and TRL half-lives and preheparin LpL suggest that MV could be a reflection of whole-body LpL binding capacity. The increases in MVs with EPA and DHA supplementation suggest that these fatty acids may increase the amount of endothelial-bound LpL or its affinity for TRL.
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Increased margination of TG-rich lipoproteins with DHA and EPA supplementation indicated that the treatment increased either the amount of endothelial bound LPL or the affinity of lipoproteins to LPL.
“The increased margination of TG-rich lipoproteins with DHA and EPA supplementation indicated that the treatment increased either the amount of endothelial bound LPL or the affinity of lipoproteins to LPL [39].”
Lowered postprandial TG levels are explained by increased chylomicron clearance.
“Lowered postprandial TG levels are also explained by increased chylomicron clearance.”
Pre-heparin LPL activity is better associated with margination of TG-rich lipoproteins as an estimation of lipoprotein activity than post-heparin activity.
“Pre-heparin activity is a measure of LPL released from endothelial cells, including endogenous plasma activators and inhibitors, and might therefore explain why pre- rather than post-heparin LPL activity is better associated with margination of TG-rich lipoproteins as an estimation of lipoprotein activity [39].”
Comparing pre-heparin and post-heparin LPL activity showed consistently increased pre-heparin activity, while post-heparin activity was unchanged by OM3FA treatment in most studies but not all.
“Comparing pre-heparin and post-heparin LPL activity showed consistently increased pre-heparin activity, while post-heparin activity was unchanged by OM3FA treatment in most studies [38, 39, 43] but not all [41].”
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- Authors
- Park, Y., Jones, P. G., & Harris, W. S.
- Affiliations
- Saint Luke's Hospital (US)
- Venue
- The American Journal of Clinical Nutrition
- Publisher
- Elsevier BV
- Published
- 2004-07
- Volume
- 80
- Issue
- 1
- Pages
- 45-50
- ISSN
- 0002-9165 (ISSN-L)
- OpenAlex
- W2159158906
- Cited by
- 41
- References
- 55
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- American Journal of Clinical Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl047468-04)
- National Heart, Lung, and Blood Institute (HL-47468)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- lipoprotein lipase, eicosapentaenoic acid, docosahexaenoic acid, omega-3 fatty acids, safflower oil, plasma volume, fasting and fed state, triglycerides, omega-3 supplementation
- MeSH terms
- Administration, Oral, Adult, Aged, Blood Volume, Docosahexaenoic Acids, Double-Blind Method, Fasting, Female, Heparin, Humans, Hyperlipidemias, Lipoprotein Lipase, Lipoproteins, Male, Metabolic Clearance Rate, Middle Aged, Safflower Oil, Triglycerides, Eicosapentaenoic Acid, Fatty Acids, Omega-3, Cross-Over Studies, Postprandial Period, Dietary Supplements
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