Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype
Open accessAbstract
We sought to test the hypothesis that dietary long-chain n-3 PUFA (LC n-3 PUFA) in fish oil stimulate the gene expression of lipoprotein lipase (LPL) in human adipose tissue (AT). In a randomized, double blind, placebo-controlled, cross-over study, 51 male subjects expressing an atherogenic lipoprotein phenotype (ALP) had their diets supplemented with fish oil for 6 weeks. As we previously reported for this group, supplementation with LC n-3 PUFA produced a decrease in fasting plasma triglyceride (TG) (-35%, P < 0.05), attenuation of the postprandial TG response (area and incremental area under the curve; AUC and IAUC, P < 0.05), and a decrease in small, dense LDL. The present study extended these observations by showing that these changes were accompanied by a marked increase in the concentration of LPL mRNA in adipose tissue (AT-LPL mRNA, +55%, P = 0.003) and post-heparin LPL activity (PH-LPL, +31%, P = 0.036). There was also evidence of an association between LPL gene expression and polymorphism in the apolipoprotein E gene. We conclude that the favorable influence of dietary n-3 PUFA on the ALP may be mediated, in part, through an increase in the plasma activity and gene expression of lipoprotein lipase in human adipose tissue.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The possibility that fish oil enhances non-LPL-mediated clearance by tissues such as spleen, muscle, adipose tissue, or kidney cannot be excluded.
“The possibility that fish oil enhances non-LPL-mediated clearance by tissues such as spleen, muscle, adipose tissue, or kidney cannot be excluded, however (16,33).”
From Omega-3 fatty acid supplementation accelerates chylomicron triglyceride clearance (2003) · cites this paper as [16]
LPL mRNA expression in adipose tissue increased in patients receiving fish oil supplementation (6 g daily for 6 weeks, men with atherogenic dyslipidemia).
“Interestingly, LPL mRNA expression in adipose tissue increased in patients receiving fish oil supplementation.”
APOE4 carriers respond to fish oil supplementation with a larger increase in LPL mRNA and post-heparin plasma LPL activity than non-APOE4 carriers.
“Furthermore, it has been shown that APOE4 carriers respond to fish oil supplementation with a larger increase in LPL mRNA and post-heparin plasma LPL activity than non-APOE4 carriers [41].”
EPA and DHA are thought to interfere with VLDL assembly and secretion and to promote apolipoprotein B degradation.
“EPA and DHA are thought to interfere with assembly and secretion of very low-density lipoprotein and promote degradation of apolipoprotein B”
From Cardiovascular Impact of Nutritional Supplementation With Omega-3 Fatty Acids (2021) · cites this paper as [46]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczyński, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 47,092 · see citing works
A simple salting out procedure for extracting DNA from human nucleated cells
Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.3.1215 · Cited by 20,341 · see citing works
Low-Density Lipoprotein Subclass Patterns and Risk of Myocardial Infarction
Austin, M. A. (1988) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.1988.03410130125037 · Cited by 1,780 · see citing works
Atherogenic lipoprotein phenotype. A proposed genetic marker for coronary heart disease risk
Austin, M. A., King, M. C., Vranizan, K. M., & Krauss, R. M. (1990) · Circulation · doi:10.1161/01.cir.82.2.495 · Cited by 1,411 · see citing works
Fish oils and plasma lipid and lipoprotein metabolism in humans: a critical review
Harris, W. S. (1989) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38310-3 · Cited by 1,127 · see citing works
REGULATION OF GENE EXPRESSION BY DIETARY FAT
Jump, D. Β., & Clarke, S. D. (1999) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.19.1.63 · Cited by 664 · see citing works
Griffin, B. A., Freeman, D. J., Tait, G. W., Thomson, J., Caslake, M. J., Packard, C. J., & Shepherd, J. (1994) · Atherosclerosis · doi:10.1016/0021-9150(94)90129-5 · Cited by 525 · see citing works
Self-citationLipoprotein Heterogeneity and Apolipoprotein B Metabolism
Packard, C. J., & Shepherd, J. (1997) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.17.12.3542 · Cited by 401 · see citing works
Weintraub, M. S., Zechner, R., Brown, A., Eisenberg, S., & Breslow, J. L. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113806 · Cited by 331 · see citing works
Reduction of postprandial triglyceridemia in humans by dietary n-3 fatty acids
Harris, W. S., Connor, W. E., Alam, N., & Illingworth, D. R. (1988) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38424-8 · Cited by 321 · see citing works
Regulation of the synthesis, processing and translocation of lipoprotein lipase
Braun, J. E., & Severson, D. L. (1992) · Biochemical Journal · doi:10.1042/bj2870337 · Cited by 320 · see citing works
Metabolic Regulation: A Human Perspective
Frayn, K. N. (1996) · Cited by 276 · see citing works
Postprandial lipemia, fenofibrate and coronary artery disease
Simpson, H., Williamson, C., Olivecrona, T., Pringle, S., MacLean, J. A., Lorimer, A. R., Bonnefous, F., Bogaievsky, Y., Packard, C. J., & Shepherd, J. (1990) · Atherosclerosis · doi:10.1016/0021-9150(90)90111-u · Cited by 229 · see citing works
ApoE Polymorphism and Fish Oil Supplementation in Subjects With an Atherogenic Lipoprotein Phenotype
Minihane, A. M., Khan, S., Leigh-Firbank, E. C., Talmud, P., Wright, J. W., Murphy, M. C., Griffin, B. A., & Williams, C. M. (2000) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.20.8.1990 · Cited by 215 · see citing works
Self-citationRaclot, T., Groscolas, R., Langin, D., & Ferré, P. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37127-3 · Cited by 213 · see citing works
Exercise induces human lipoprotein lipase gene expression in skeletal muscle but not adipose tissue
Seip, R. L., Angelopoulos, T. J., & Semenkovich, C. F. (1995) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1995.268.2.e229 · Cited by 190 · see citing works
Enerbäck, S., & Gimble, J. M. (1993) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(93)90196-g · Cited by 185 · see citing works
Arai, T., Yamashita, S., Hirano, K., Sakai, N., Kotani, K., Fujioka, S., Nozaki, S., Keno, Y., Yamane, M., & Shinohara, E. (1994) · Arteriosclerosis and Thrombosis A Journal of Vascular Biology · doi:10.1161/01.atv.14.7.1129 · Cited by 174 · see citing works
Zechner, R. (1997) · Current Opinion in Lipidology · doi:10.1097/00041433-199704000-00005 · Cited by 152 · see citing works
Familial combined hyperlipidemia and abnormal lipoprotein lipase.
Babirak, S. P., Brown, B. G., & Brunzell, J. D. (1992) · Arteriosclerosis and Thrombosis A Journal of Vascular Biology · doi:10.1161/01.atv.12.10.1176 · Cited by 146 · see citing works
Relation of components of insulin resistance syndrome to coronary disease risk
rs, J.-P. D., & Marette, A. (1994) · Current Opinion in Lipidology · doi:10.1097/00041433-199408000-00006 · Cited by 142 · see citing works
Influence of n-3 fatty acid supplementation on the endogenous activities of plasma lipases
Harris, W., Lu, G., Rambjør, G., Wõlen, A., Ontko, J., Cheng, Q., & Windsor, S. (1997) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/66.2.254 · Cited by 135 · see citing works
Kiens, B., Essén-Gustavsson, B., Gad, P., & Lithell, H. (1987) · Clinical Physiology · doi:10.1111/j.1475-097x.1987.tb00628.x · Cited by 134 · see citing works
Laville, M., Auboeuf, D., Khalfallah, Y., Véga, N., Riou, J. P., & Vidal, H. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118775 · Cited by 121 · see citing works
Luo, J., Rizkalla, S. W., Vidal, H., Oppert, J., Colas, C., Boussairi, A., Guerre-Millo, M., Chapuis, A.-S., Chevalier, A., Durand, G., et al. (1998) · Diabetes Care · doi:10.2337/diacare.21.5.717 · Cited by 114 · see citing works
Bolla, M. K., Haddad, L., Humphries, S. E., WINDER, A. F., & Day, I. N. M. (1995) · Clinical Chemistry · doi:10.1093/clinchem/41.11.1599 · Cited by 90 · see citing works
Henderson, H., Kastelein, J. J. P., Zwinderman, A. H., Gagné, E., Jukema, J. W., Reymer, P. W. A., Groenemeyer, B. E., Lie, K. I., Bruschke, A. V. G., Hayden, M. R., et al. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32153-2 · Cited by 87 · see citing works
Changes of lipolytic enzymes cluster with insulin resistance syndrome
Knudsen, P., Eriksson, J., Lahdenper, S., Kahri, J., Groop, L., Taskinen, M., & The Botnia Study Group (1995) · Diabetologia · doi:10.1007/bf00400640 · Cited by 87 · see citing works
Öhrvall, M., Lithell, H., Johansson, J., & Vessby, B. (1995) · Metabolism · doi:10.1016/0026-0495(95)90267-8 · Cited by 86 · see citing works
Clarke, S. D., Turini, M. E., & Jump, D. Β. (1997) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(97)90494-4 · Cited by 74 · see citing works
Amri, E., Teboul, L., Vannier, C., et al. (1996) · Biochemical Journal · doi:10.1042/bj3140541 · Cited by 73 · see citing works
Nozaki, S., Garg, A., Vega, G., & Grundy, S. M. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/53.3.638 · Cited by 67 · see citing works
Murphy, M. C., Zampelas, A., Puddicombe, S. M., Furlonger, N., Morgan, L., & Williams, C. M. (1993) · British Journal Of Nutrition · doi:10.1079/bjn19930168 · Cited by 40 · see citing works
Self-citationSemb, H., & Olivecrona, T. (1989) · Biochemical Journal · doi:10.1042/bj2620505 · Cited by 38 · see citing works
Kendrick, J. S., & Higgins, J. A. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32455-x · Cited by 34 · see citing works
Murphy, M. C., Brooks, C., Rockett, J. C., Chapman, C. M. L., Lovegrove, J. A., GOULD, B. J., Wright, J., & Williams, C. M. (1999) · European Journal of Clinical Nutrition · doi:10.1038/sj.ejcn.1600774 · Cited by 11 · see citing works
Self-citationRole of N‐6 and N‐3 Fatty Acids in the Dietary Treatment of Metabolic Disorders
Collier, G. R., & Sinclair, A. J. (1993) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1993.tb35723.x · Cited by 10 · see citing works
Zampelas, A., Morgan, L., Murphy, M. C., & Williams, C. M. (1994) · Cited by 4 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Omega-3 fatty acids and coronary heart disease risk: clinical and mechanistic perspectives
Harris, W. S., Miller, M., Tighe, A. P., Davidson, M. H., & Schaefer, E. J. (2008) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2007.11.008 · Cited by 552 · see citing works
Why do omega-3 fatty acids lower serum triglycerides?
Harris, W. S., & Bulchandani, D. (2006) · Current Opinion in Lipidology · doi:10.1097/01.mol.0000236363.63840.16 · Cited by 398 · see citing works
Omega-3 fatty acid supplementation accelerates chylomicron triglyceride clearance
Park, Y., & Harris, W. S. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200282-jlr200 · Cited by 348 · see citing works
Fish oil — How does it reduce plasma triglycerides?
Shearer, G. C., Savinova, O. V., & Harris, W. S. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2011.10.011 · Cited by 285 · see citing works
Severe hypertriglyceridemia and pancreatitis: presentation and management
Ewald, N., Hardt, P. D., & Kloer, H.-U. (2009) · Current Opinion in Lipidology · doi:10.1097/mol.0b013e3283319a1d · Cited by 273 · see citing works
Kastelein, J. J. P., Maki, K. C., Susekov, A., Ezhov, M., Nordestgaard, B. G., Machielse, B. N., Kling, D., & Davidson, M. H. (2014) · Journal of Clinical Lipidology · doi:10.1016/j.jacl.2013.10.003 · Cited by 233 · see citing works
The clinical relevance of omega-3 fatty acids in the management of hypertriglyceridemia
Backes, J., Anzalone, D., Hilleman, D., & Catini, J. (2016) · Lipids in Health and Disease · doi:10.1186/s12944-016-0286-4 · Cited by 195 · see citing works
Severe hypertriglyceridemia in pregnancy
Goldberg, A. S., & Hegele, R. A. (2012) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2012-1250 · Cited by 187 · see citing works
Oscarsson, J., & Hurt-Camejo, E. (2017) · Lipids in Health and Disease · doi:10.1186/s12944-017-0541-3 · Cited by 158 · see citing works
Buckley, R., Shewring, B., Turner, R., Yaqoob, P., & Minihane, A. M. (2004) · British Journal of Nutrition · doi:10.1079/bjn20041235 · Cited by 118 · see citing works
Self-citationOmega-3 fatty acid concentrates in the treatment of moderate hypertriglyceridemia
Skulas-Ray, A. C., West, S. G., Davidson, M. H., & Kris-Etherton, P. M. (2008) · Expert Opinion on Pharmacotherapy · doi:10.1517/14656566.9.7.1237 · Cited by 91 · see citing works
Olano-Martin, E., Anil, E., Caslake, M. J., Packard, C. J., Bedford, D., Stewart, G., Peiris, D., Williams, C. M., & Minihane, A. M. (2010) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2009.08.024 · Cited by 83 · see citing works
Self-citationCardiovascular Impact of Nutritional Supplementation With Omega-3 Fatty Acids
Weinberg, R. L., Brook, R. D., Rubenfire, M., & Eagle, K. A. (2021) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2020.11.060 · Cited by 63 · see citing works
Bays, H. E., McKenney, J., Maki, K. C., Doyle, R. T., Carter, R. N., & Stein, E. (2010) · Mayo Clinic Proceedings · doi:10.4065/mcp.2009.0397 · Cited by 54 · see citing works
Park, Y., Jones, P. G., & Harris, W. S. (2004) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/80.1.45 · Cited by 41 · see citing works
Rudkowska, I., Caron‐Dorval, D., Verreault, M., Couture, P., Deshaies, Y., Barbier, O., & Vohl, M. (2010) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200900085 · Cited by 36 · see citing works
Ouguerram, K., Maugeais, C., Gardette, J., Magot, T., & Krempf, M. (2006) · British Journal of Nutrition · doi:10.1079/bjn20061806 · Cited by 31 · see citing works
Controversies in the Use of Omega-3 Fatty Acids to Prevent Atherosclerosis
Quispe, R., Alfaddagh, A., Kazzi, B., Zghyer, F., Marvel, F. A., Blumenthal, R. S., Sharma, G., & Martin, S. S. (2022) · Current Atherosclerosis Reports · doi:10.1007/s11883-022-01031-9 · Cited by 17 · see citing works
<p>Spotlight on Icosapent Ethyl for Cardiovascular Risk Reduction: Evidence to Date</p>
Jia, X., Koh, S., Al Rifai, M., Blumenthal, R. S., & Virani, S. S. (2020) · Vascular Health and Risk Management · doi:10.2147/vhrm.s210149 · Cited by 14 · see citing works
- Authors
- Khan, S., Minihane, A.-M., Talmud, P. J., Wright, J. W., Murphy, M. C., Williams, C. M., & Griffin, B. A.
- Affiliations
- University of Surrey (GB)
- University of Reading (GB)
- University College London (GB)
- Corresponding author
- Griffin, Bruce A.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2002-06
- Volume
- 43
- Issue
- 6
- Pages
- 979-985
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2151651725
- Cited by
- 145
- References
- 39
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 3 · 2022: 5 · 2021: 9 · 2020: 9 · 2019: 7 · 2018: 7 · 2017: 4 · 2016: 5 · 2015: 5 · 2014: 4 · 2013: 5 · 2012: 11
- FWCI
- 4.35
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- UEA Digital Repository (University of East Anglia) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- n-3 long-chain polyunsaturated fatty acids, fish oil supplementation, lipoprotein lipase, adipose tissue gene expression, atherogenic lipoprotein phenotype, APOE polymorphism, plasma triglycerides, postprandial triglyceride response, small dense LDL, dietary fatty acids
- MeSH terms
- Adipose Tissue, Apolipoproteins E, Blood Platelets, Cell Membrane, Dietary Fats, Double-Blind Method, Fatty Acids, Fatty Acids, Unsaturated, Fish Oils, Humans, Lipids, Lipoprotein Lipase, Lipoproteins, RNA, Messenger, Cross-Over Studies, Lipid Metabolism
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
