Isolation of a New Member of the Ecdysteroid Glycoside Family: 2-Deoxy-20-hydroxyecdysone 22-O- -D-Glucopyranoside
Open accessAbstract
A new ecdysteroid glycoside, 2-deoxy-20-hydroxyecdysone 22-O-beta-D-glucopyranoside, is isolated from the herb Silene italica ssp. nemoralis (Waldst. and Kit.) Nyman. The compound is purified with multistep chromatography, such as classical column chromatography on alumina and droplet countercurrent distribution. Also, it is expanded using twice low-pressure reversed-phase liquid column chromatography. Chromatography in four steps results in the purified 2-deoxy-20-hydroxyecdysone 22-O-beta-D-glucopyranoside. Two other ecdysteroids have also been separated, including the formerly identified integristerone A and 24(28)-dehydromakisterone A.
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DCCC is an effective technique for the purification of ecdysteroids of wide polarity range from complex mixtures.
“DCCC, which is a preparative partition system between immiscible stationary and mobile phases, is an effective technique for the purification of ecdysteroids of wide polarity range from complex mixtures [119-121] since it is non-destructive and all components elute at some point during the chromatography”
From Ecdysteroids (2021) · cites this paper as [120]
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- Authors
- Bathori, M., Pongracz, Z., Toth, G., Simon, A., Kandra, L., Kele, Z., & Ohmacht, R.
- Affiliations
- Budapest University of Technology and Economics (HU)
- University of Debrecen (HU)
- University of Szeged (HU)
- University of Pecs (HU)
- Venue
- Journal of Chromatographic Science
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-08-01
- Volume
- 40
- Issue
- 7
- Pages
- 409-415
- ISSN
- 0021-9665 (ISSN-L)
- 1945-239X
- OpenAlex
- W2115423976
- Cited by
- 23
- References
- 6
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2014: 1 · 2012: 9
- FWCI
- 0.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Chromatographic Science (publishedVersion)
- PubMed (publishedVersion)
- University of Debrecen Electronic Archive (University of Debrecen) (submittedVersion)
- Funders
- Hungarian Scientific Research Fund (T032185)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- natural product isolation, ecdysteroids, plant secondary metabolites, chromatographic purification
- MeSH terms
- Chromatography, Liquid, Glycosides, Monosaccharides, Mass Spectrometry, Steroids, Ecdysteroids, Caryophyllaceae
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