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Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol.
Abstract
We compared the standard Lipid Research Clinics heparin-Mn2+ (46 mmol/L) method and five improved precipitation methods for quantification of high-density lipoprotein (HDL) cholesterol. Three of these methods--a dextran sulfate-Mg2+ procedure, reported as a Selected Method, a modified heparin-Mn2+ (92 mmol/L) method, and a modified phosphotungstate-Mg2+ procedure--all gave similar results. Three other methods--the standard heparin-Mn2+ (46 mmol/L) method and two polyethylene glycol methods (75 g/L or pH 10 reagent at 100 g/L final concentrations)--gave slightly higher values for HDL cholesterol. Addition of NaCl or glucose to specimens did not significantly change protein precipitation. In terms of sedimentation effectiveness with hypertriglyceridemic specimens, the methods were ranked in the following order: polyethylene glycol (pH 10, 100 g/L) greater than dextran sulfate-Mg2+ greater than heparin-Mn2+ (92 mmol/L) = polyethylene glycol (75 g/L) greater than phosphotungstate-Mg2+ greater than heparin-Mn2+ (46 mmol/L).
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After dextran sulfate precipitation, there were no differences in lipoprotein particle concentrations.
“We repeated the ion mobility analyses in plasma after treatment with dextran sulfate, which precipitates ApoB and ApoE containing particles26, and consistent with the increased ApoE identified in the FPLC fractions, found no differences in particle concentrations (Fig. SVIC).”
From Phosphatidylinositol-(4,5)-Bisphosphate Regulates Plasma Cholesterol Through LDL (Low-Density Lipoprotein) Receptor Lysosomal Degradation (2020) · cites this paper as [26]
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Metadata
- Authors
- Warnick, G. R., Nguyen, T., & Albers, A. A.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1985-02-01
- Volume
- 31
- Issue
- 2
- Pages
- 217-222
- ISSN
- 0009-9147
- 1530-8561
- Cited by
- 303
- References
- 0
- Language
- en
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