Lesion bypass in yeast cells: Pol η participates in a multi‐DNA polymerase process
Open accessAbstract
Replication through (6-4)TT and G-AAF lesions was compared in Saccharomyces cerevisiae strains proficient and deficient for the RAD30-encoded DNA polymerase eta (Pol eta). In the RAD30 strain, the (6-4)TT lesion is replicated both inaccurately and accurately 60 and 40% of the time, respectively. Surprisingly, in a rad30 Delta strain, the level of mutagenic bypass is essentially suppressed, while error-free bypass remains unchanged. Therefore, Pol eta is responsible for mutagenic replication through the (6-4)TT photoproduct, while another polymerase mediates its error-free bypass. Deletion of the RAD30 gene also reduces the levels of both accurate and inaccurate bypass of AAF lesions within two different sequence contexts up to 8-fold. These data show that, in contrast to the accurate bypass by Pol eta of TT cyclobutane dimers, it is responsible for the mutagenic bypass of other lesions. In conclusion, this paper shows that, in yeast, translesion synthesis involves the combined action of several polymerases.
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Polη may also be involved in bypass of 6-4PPs, as suggested by plasmid assays in yeast and human cells.
“Pol may also be involved in the bypass of 6-4PPs as suggested by plasmids assays performed both in yeast and human cells [26,27].”
From Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome (2014) · cites this paper as [27]
Some data suggest Polη can also play a role in bypass of 6-4PPs.
“some data suggest that Pol can also play a role in the bypass of 6-4PPs [26,27]”
From Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome (2014) · cites this paper as [27]
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- Authors
- Bresson, A., & Fuchs, R. P. P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Fuchs, Robert P.P.
- ORCID
- Fuchs, Robert P.P.
- Venue
- The EMBO Journal
- Published
- 2002-07-15
- Volume
- 21
- Issue
- 14
- Pages
- 3881-3887
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC126109
- OpenAlex
- W2009214067
- Cited by
- 112
- References
- 43
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 6 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 5 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 10
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA polymerase eta, translesion synthesis, error-free bypass, cyclobutane pyrimidine dimers, DNA repair, Saccharomyces cerevisiae
- MeSH terms
- Y-Family DNA Polymerases, DNA-Directed DNA Polymerase, Saccharomyces cerevisiae
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