Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast
Open accessAbstract
Unrepaired DNA lesions in the template strand block the replication fork. In yeast, Mec1 protein kinase-mediated replication checkpoint prevents the breakdown of replication forks and maintains viability in DNA-damaged cells going through the S phase. By ensuring that the replisome does not dissociate from the fork stalled at the lesion site, the replication checkpoint presumably coordinates the action of lesion bypass processes with the replisome. However, it has remained unclear as to which of the lesion bypass processes—translesion synthesis (TLS) and/or template switching—depend on the activation of the replication checkpoint. Here we determine if the Mec1 kinase and the subunits of the checkpoint clamp and the clamp loader are required for TLS. We show that proficient TLS can occur in the absence of these checkpoint proteins in nucleotide excision repair (NER)-proficient cells; however, in the absence of NER, checkpoint protein-mediated Rev1 phosphorylation contributes to increasing the proficiency of DNA polymerase ζ-dependent TLS.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
An active checkpoint leads to Rev1 phosphorylation, possibly increasing TLS activity and reducing RPA-covered ssDNA, promoting checkpoint switch-off.
“An active checkpoint leads to Rev1 phosphorylation [86,87], possibly increasing TLS activity and progressively reducing the amount of RPA-covered ssDNA, thus promoting the switch off of the checkpoint itself.”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [86]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Gietz, R. D., & Sugino, A. (1988) · Gene · doi:10.1016/0378-1119(88)90185-0 · Cited by 2,901 · see citing works
RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
Hoege, C., Pfander, B., Moldovan, G., Pyrowolakis, G., & Jentsch, S. (2002) · Nature · doi:10.1038/nature00991 · Cited by 2,202 · see citing works
Sancar, A. (1996) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.65.070196.000355 · Cited by 1,131 · see citing works
EUKARYOTIC TRANSLESION SYNTHESIS DNA POLYMERASES: Specificity of Structure and Function
Prakash, S., Johnson, R. E., & Prakash, L. (2005) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133250 · Cited by 999 · see citing works
Self-citationControl of spontaneous and damage-induced mutagenesis by SUMO and ubiquitin conjugation
Stelter, P., & Ulrich, H. D. (2003) · Nature · doi:10.1038/nature01965 · Cited by 849 · see citing works
Fork Reversal and ssDNA Accumulation at Stalled Replication Forks Owing to Checkpoint Defects
Sogo, J. M., Lopes, M., & Foiani, M. (2002) · Science · doi:10.1126/science.1074023 · Cited by 824 · see citing works
Toward Maintaining the Genome: DNA Damage and Replication Checkpoints
Nyberg, K. A., Michelson, R. J., Putnam, C. W., & Weinert, T. A. (2002) · Annual Review of Genetics · doi:10.1146/annurev.genet.36.060402.113540 · Cited by 770 · see citing works
Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Polη
Johnson, R. E., Prakash, S., & Prakash, L. (1999) · Science · doi:10.1126/science.283.5404.1001 · Cited by 736 · see citing works
Self-citationRegulation of DNA replication fork progression through damaged DNA by the Mec1/Rad53 checkpoint
Tercero, J. A., & Diffley, J. F. X. (2001) · Nature · doi:10.1038/35087607 · Cited by 667 · see citing works
Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ
Nelson, J. R., Lawrence, C. W., & Hinkle, D. C. (1996) · Science · doi:10.1126/science.272.5268.1646 · Cited by 654 · see citing works
Eukaryotic polymerases ι and ζ act sequentially to bypass DNA lesions
Johnson, R. E., Washington, M. T., Haracska, L., Prakash, S., & Prakash, L. (2000) · Nature · doi:10.1038/35023030 · Cited by 632 · see citing works
Self-citationDeoxycytidyl transferase activity of yeast REV1 protein
Nelson, J. R., Lawrence, C. W., & Hinkle, D. C. (1996) · Nature · doi:10.1038/382729a0 · Cited by 573 · see citing works
Fidelity of Human DNA Polymerase η
Johnson, R. E., Washington, M. T., Prakash, S., & Prakash, L. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.11.7447 · Cited by 399 · see citing works
Self-citationA Central Role for DNA Replication Forks in Checkpoint Activation and Response
Tercero, J. A., Longhese, M. P., & Diffley, J. F. X. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00169-2 · Cited by 394 · see citing works
Developmental Control of Cell Cycle Regulators: A Fly's Perspective
Edgar, B. A., & Lehner, C. F. (1996) · Science · doi:10.1126/science.274.5293.1646 · Cited by 389 · see citing works
DNA polymerase stabilization at stalled replication forks requires Mec1 and the RecQ helicase Sgs1
Cobb, J. A., Bjergbæk, L., Shimada, K., Frei, C., & Gasser, S. M. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg391 · Cited by 333 · see citing works
You, Y.-H., Lee, D.-H., Yoon, J.-H., Nakajima, S., Yasui, A., & Pfeifer, G. P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m107696200 · Cited by 314 · see citing works
McDonald, J. P., Levine, A. S., & Woodgate, R. (1997) · Genetics · doi:10.1093/genetics/147.4.1557 · Cited by 314 · see citing works
Prakash, L. (1981) · Molecular and General Genetics MGG · doi:10.1007/bf00352525 · Cited by 291 · see citing works
Self-citationBlastyák, A., Pintér, L., Unk, I., Prakash, L., Prakash, S., & Haracska, L. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.07.030 · Cited by 285 · see citing works
Self-citationThe ATR pathway: Fine-tuning the fork
PAULSEN, R., & Cimprich, K. A. (2007) · DNA repair · doi:10.1016/j.dnarep.2007.02.015 · Cited by 256 · see citing works
Yeast Rad17/Mec3/Ddc1: A sliding clamp for the DNA damage checkpoint
Majka, J., & Burgers, P. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0437148100 · Cited by 255 · see citing works
Haracska, L., Torres‐Ramos, C. A., Johnson, R. E., Prakash, S., & Prakash, L. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.10.4267-4274.2004 · Cited by 201 · see citing works
Self-citationInteraction with PCNA Is Essential for Yeast DNA Polymerase η Function
Haracska, L., Kondratick, C. M., Unk, I., Prakash, S., & Prakash, L. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00319-7 · Cited by 198 · see citing works
Self-citationCobb, J. A., Schleker, T., Rojas, V., Bjergbæk, L., Tercero, J. A., & Gasser, S. M. (2005) · Genes & Development · doi:10.1101/gad.361805 · Cited by 197 · see citing works
Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase eta
Washington, M. T., Johnson, R. E., Prakash, S., & Prakash, L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.050491997 · Cited by 173 · see citing works
Self-citationTorres‐Ramos, C. A., Prakash, S., & Prakash, L. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.7.2419-2426.2002 · Cited by 170 · see citing works
Self-citationHuman DINB1 -encoded DNA polymerase κ is a promiscuous extender of mispaired primer termini
Washington, M. T., Johnson, R. E., Prakash, L., & Prakash, S. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.032594399 · Cited by 165 · see citing works
Self-citationYeast Rev1 Protein Is a G Template-specific DNA Polymerase
Haracska, L., Prakash, S., & Prakash, L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112146200 · Cited by 164 · see citing works
Self-citationCheckpoint activation regulates mutagenic translesion synthesisPreprint
Kai, M., & Wang, T. S. ‐. (2003) · Genes & Development · doi:10.1101/gad.1043203 · Cited by 157 · see citing works
Paulovich, A. G., Armour, C. D., & Hartwell, L. H. (1998) · Genetics · doi:10.1093/genetics/150.1.75 · Cited by 133 · see citing works
Requirement of DNA Polymerase η for Error-Free Bypass of UV-Induced CC and TC Photoproducts
Yu, S.-L., Johnson, R. E., Prakash, S., & Prakash, L. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.1.185-188.2001 · Cited by 133 · see citing works
Self-citationAcharya, N., Johnson, R. E., Prakash, S., & Prakash, L. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.01671-06 · Cited by 128 · see citing works
Self-citationZhuang, Z., Johnson, R. E., Haracska, L., Prakash, L., Prakash, S., & Benkovic, S. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801310105 · Cited by 121 · see citing works
Self-citationSabbioneda, S., Minesinger, B. K., Giannattasio, M., Plevani, P., Muzi-Falconi, M., & Jinks-Robertson, S. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m507638200 · Cited by 116 · see citing works
Gangavarapu, V., Haracska, L., Unk, I., Johnson, R. E., Prakash, S., & Prakash, L. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.01260-06 · Cited by 114 · see citing works
Self-citationLesion bypass in yeast cells: Pol η participates in a multi‐DNA polymerase process
Bresson, A., & Fuchs, R. P. P. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf363 · Cited by 112 · see citing works
Acharya, N., Yoon, J.-H., Gali, H., Unk, I., Haracska, L., Johnson, R. E., Hurwitz, J., Prakash, L., & Prakash, S. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0809844105 · Cited by 111 · see citing works
Self-citationNeecke, H., Lucchini, G., & Pia Longhese, M. (1999) · The EMBO Journal · doi:10.1093/emboj/18.16.4485 · Cited by 94 · see citing works
Gangavarapu, V., Prakash, S., & Prakash, L. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01331-07 · Cited by 92 · see citing works
Self-citationMechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase η
Washington, M. T., Prakash, L., & Prakash, S. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2134223100 · Cited by 76 · see citing works
Self-citationSabbioneda, S., Bortolomai, I., Giannattasio, M., Plevani, P., & Muzi-Falconi, M. (2007) · DNA Repair · doi:10.1016/j.dnarep.2006.09.002 · Cited by 65 · see citing works
Nair, D. T., Johnson, R. E., Prakash, L., Prakash, S., & Aggarwal, A. K. (2008) · Structure · doi:10.1016/j.str.2007.12.009 · Cited by 64 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Y-family DNA polymerases and their role in tolerance of cellular DNA damage
Sale, J. E., Lehmann, A. R., & Woodgate, R. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3289 · Cited by 665 · see citing works
Translesion DNA Synthesis and Mutagenesis in Eukaryotes
Sale, J. E. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a012708 · Cited by 282 · see citing works
Karras, G. I., & Jentsch, S. (2010) · Cell · doi:10.1016/j.cell.2010.02.028 · Cited by 282 · see citing works
Despras, E., Daboussi, F., Hyrien, O., Marheineke, K., & Kannouche, P. L. (2010) · Human Molecular Genetics · doi:10.1093/hmg/ddq046 · Cited by 80 · see citing works
Mind the gap: keeping UV lesions in check
Novarina, D., Amara, F., Lazzaro, F., Plevani, P., & Muzi-Falconi, M. (2011) · DNA Repair · doi:10.1016/j.dnarep.2011.04.030 · Cited by 43 · see citing works
“An active checkpoint leads to Rev1 phosphorylation [86,87], possibly increasing TLS activity and progressively reducing the amount of RPA-covered ssDNA, thus promoting the switch off of the checkpoint itself.”
Background
- Authors
- Pagès, V., Santa Maria, S. R., Prakash, L., & Prakash, S.
- Affiliations
- The University of Texas Medical Branch at Galveston (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2009-06-15
- Volume
- 23
- Issue
- 12
- Pages
- 1438-1449
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC2701570
- OpenAlex
- W2156568122
- Cited by
- 55
- References
- 43
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 1 · 2016: 3 · 2015: 6 · 2014: 1 · 2013: 4 · 2012: 7
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Institutes of Health (CA107650)
- National Cancer Institute (R01CA107650, CA107650)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- DNA damage, S-phase checkpoint, translesion synthesis, lesion bypass, Mec1 kinase, clamp loader, DNA polymerase zeta, nucleotide excision repair, template switching, replication fork stalling, yeast
- MeSH terms
- Y-Family DNA Polymerases, DNA Damage, DNA Repair, DNA Replication, DNA, Bacterial, Nucleotidyltransferases, Phosphoproteins, Phosphorylation, Saccharomyces cerevisiae, Ultraviolet Rays, Mutagenesis, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins, Adaptor Proteins, Signal Transducing
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
