Roles of Saccharomyces cerevisiae DNA polymerases Pol and Pol in response to irradiation by simulated sunlight
Open accessAbstract
Sunlight causes lesions in DNA that if unrepaired and inaccurately replicated by DNA polymerases yield mutations that result in skin cancer in humans. Two enzymes involved in translesion synthesis (TLS) of UV-induced photolesions are DNA polymerase eta (Poleta) and polymerase zeta (Polzeta), encoded by the RAD30A and REV3 genes, respectively. Previous studies have investigated the TLS roles of these polymerases in human and yeast cells irradiated with monochromatic, short wavelength UVC radiation (254 nm). However, less is known about cellular responses to solar radiation, which is of higher and mixed wavelengths (310-1100 nm) and produces a different spectrum of DNA lesions, including Dewar photoproducts and oxidative lesions. Here we report on the comparative cytotoxic and mutagenic effects of simulated sunlight (SSL) and UVC radiation on yeast wild-type, rad30Delta, rev3Delta and rev3Delta rad30Delta strains. The results with SSL support several previous interpretations on the roles of these two polymerases in TLS of photodimers and (6-4) photoproducts derived from studies with UVC. They further suggest that Poleta participates in the non-mutagenic bypass of SSL-dependent cytosine-containing Dewar photoproducts and 8-oxoguanine, while Polzeta is mainly responsible for the mutagenic bypass of all types of Dewar photoproducts. They also suggest that in the absence of Polzeta, Poleta contributes to UVC- and SSL-induced mutagenesis, possibly by the bypass of photodimers containing deaminated cytosine.
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A previously published study in Saccharomyces cerevisiae exposed to simulated sunlight supports the role of Pol eta in TLS of CPDs and 6-4PPs.
“A previously published study in which Saccharomyces cerevisiae was exposed to simulated sunlight supports the role of Pol eta in TLS of CPDs and 6-4PPs [52].”
From Sunlight damage to cellular DNA: focus on oxidatively generated lesions (2017) · cites this paper as [52]
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- Authors
- Kozmin, S. G.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Kozmin, S. G.
- ORCID
- Kozmin, S. G.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-08-01
- Volume
- 31
- Issue
- 15
- Pages
- 4541-4552
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC169879
- OpenAlex
- W2131412000
- Cited by
- 78
- References
- 60
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 5 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 5 · 2012: 1
- FWCI
- 1.73
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nucleic Acids Research (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Association pour la Recherche sur le Cancer
- Centre National de la Recherche Scientifique
- National Institutes of Health
- National Institute of Environmental Health Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- Saccharomyces cerevisiae, DNA polymerase eta, DNA polymerase zeta, translesion synthesis, simulated sunlight, UV-C radiation, photodimers, 8-oxoguanine, mutagenesis, cytotoxicity, DNA repair, skin cancer, REV3
- MeSH terms
- Y-Family DNA Polymerases, Base Sequence, Cell Survival, Cytosine, DNA Damage, DNA-Directed DNA Polymerase, DNA Repair, DNA, Fungal, Genes, Fungal, Guanine, Light, Molecular Sequence Data, Mutation, Pyrimidine Dimers, Saccharomyces cerevisiae, Mutagenesis, Gene Deletion, Saccharomyces cerevisiae Proteins
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