The DOI matches its Crossref record; 38 of the 39 references it cites were found in a registry or an index.
The heterochromatin-associated protein HP-1 is an essential protein in Drosophila with dosage-dependent effects on position-effect variegation.
Abstract
Chromosome rearrangements which place euchromatic genes adjacent to a heterochromatic breakpoint frequently result in gene repression (position-effect variegation). This repression is thought to reflect the spreading of a heterochromatic structure into neighboring euchromatin. Two allelic dominant suppressors of position-effect variegation were found to contain mutations within the gene encoding the heterochromatin-specific chromosomal protein HP-1. The site of mutation for each allele is given: one converts Lys169 into a nonsense (ochre) codon, while the other is a frameshift after Ser10. In flies heterozygous for one of the mutant alleles (Su(var)2-504), a truncated HP-1 protein was detectable by Western blot analysis. An HP-1 minigene, consisting of HP-1 cDNA under the control of an Hsp70 heat-inducible promoter, was transduced into flies by P element-mediated germ line transformation. Heat-shock driven expression of this minigene results in elevated HP-1 protein level and enhancement of position-effect variegation. Levels of variegating gene expression thus appear to depend upon the level of expression of a heterochromatin-specific protein. The implications of these observations for mechanism of heterochromatic position effects and heterochromatin function are discussed.
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“Reduced dosage of Drosophila HP1 and Su(var)3-9 results in suppression of position-effect variegation (PEV) [19–21].”
From Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling (2014) · cites this paper as [20]
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., & Green, M. R. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.18.7035 · Cited by 6,324
Wahl, G. M., Stern, M., & Stark, G. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.8.3683 · Cited by 3,159
Genetic Transformation of Drosophila with Transposable Element Vectors
Rubin, G. M., & Spradling, A. C. (1982) · Science · doi:10.1126/science.6289436 · Cited by 2,996
Genetic variations of Drosophila melanogaster.
Lindsley, D. L., & Grell, E. H. (1968) · Biodiversity Heritage Library (Smithsonian Institution) · doi:10.5281/zenodo.10755360 · Cited by 2,623
Drosophila: A laboratory handbook
Ashburner, M. (1990) · Trends in Ecology & Evolution · doi:10.1016/0169-5347(90)90121-s · Cited by 1,751
Analysis of P transposable element functions in drosophila
Karess, R. E., & Rubin, G. M. (1984) · Cell · doi:10.1016/0092-8674(84)90534-8 · Cited by 731
James, T. C., & Elgin, S. C. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.11.3862 · Cited by 643
Functional cDNA libraries from Drosophila embryos
Brown, N. H., & Kafatos, F. C. (1988) · Journal of Molecular Biology · doi:10.1016/0022-2836(88)90010-1 · Cited by 602
Eissenberg, J. C., James, T. C., Foster-Hartnett, D. M., Hartnett, T., Ngan, V, & Elgin, S. C. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.24.9923 · Cited by 588
Self-citationParo, R., & Hogness, D. S. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.1.263 · Cited by 562
James, T. C., Eissenberg, J. C., Craig, C., Dietrich, Hobson, A., & Elgin, S. C. R. (1989) · PubMed · Cited by 407
Self-citationLOCKE, J. L., Kotarski, M. A., & Tartof, K. D. (1988) · Genetics · doi:10.1093/genetics/120.1.181 · Cited by 313
In vivo binding pattern of a trans-regulator of homoeotic genes in Drosophila melanogaster
Zink, B., & Paro, R. (1989) · Nature · doi:10.1038/337468a0 · Cited by 304
A transposable P vector that confers selectable G418 resistance to Drosophila larvae
Steller, H., & Pirrotta, V. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb02332.x · Cited by 286
Reuter, G., Giarrè, M., Farah, J. A., Gausz, J., Spierer, A., & Spierer, P. (1990) · Nature · doi:10.1038/344219a0 · Cited by 223
Self-citationThe genetics of position — effect variegation modifying loci inDrosophila melanogaster
Wustmann, G., Szidonya, J., Taubert, H., & Reuter, G. (1989) · Molecular and General Genetics MGG · doi:10.1007/bf02464926 · Cited by 169
Self-citationMolecular organization of a Drosophila puff site that responds to ecdysone
Meyerowitz, E. M., & Hogness, D. S. (1982) · Cell · doi:10.1016/0092-8674(82)90386-5 · Cited by 166
Reuter, G., & Wolff, I. (1981) · Molecular and General Genetics MGG · doi:10.1007/bf00293947 · Cited by 137
Self-citationPosition effect variegation in Drosophila: Towards a genetics of chromatin assembly
Eissenberg, J. C. (1989) · BioEssays · doi:10.1002/bies.950110105 · Cited by 131
Self-citationKGB: a single buffer for all restriction endonucleases
McClelland, M., Hanish, J., Nelson, M. B., & Patel, Y. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.1.364 · Cited by 128
Genes which suppress position-effect variegation in Drosophila melanogaster are clustered
Sinclair, D. A. R., Mottus, R. C., & Grigliatti, T. A. (1983) · Molecular and General Genetics MGG · doi:10.1007/bf00334834 · Cited by 127
Ephrussi, B., & Herold, J. L. (1944) · Genetics · doi:10.1093/genetics/29.2.148 · Cited by 118
Phillips, M., & Shearn, A. (1990) · Genetics · doi:10.1093/genetics/125.1.91 · Cited by 111
Genetic Control of Body Segment Differentiation in Drosophila
Duncan, I., & Lewis, E. B. (2007) · Springer eBooks · doi:10.1007/978-1-4020-6345-9_11 · Cited by 91
Position-effect variegation and chromosome structure of a heat shock puff in Drosophila
Henikoff, S. (1981) · Chromosoma · doi:10.1007/bf00327360 · Cited by 75
ON BIOLOGICAL FUNCTIONS MAPPING TO THE HETEROCHROMATIN OF DROSOPHILA MELANOGASTER
Pimpinelli, S., Sullivan, W., Prout, M., & Sandler, L. (1985) · Genetics · doi:10.1093/genetics/109.4.701 · Cited by 75
Mutants affecting position-effect heterochromatinization in Drosophila melanogaster
Reuter, G., Werner, W., & Hoffmann, H. J. (1982) · Chromosoma · doi:10.1007/bf00327349 · Cited by 74
Self-citationSandler, L. (1977) · Genetics · doi:10.1093/genetics/86.3.567 · Cited by 60
Tartof, K. D., & Bremer, M. (1990) · Development · doi:10.1242/dev.108.supplement.35 · Cited by 47
DNase I hypersensitive sites of the chromatin for Drosophila melanogaster ribosomal protein 49 gene
Wong, Y., O'Connell, P., Rosbash, M., & Elgin, S. C. R. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.24.6749 · Cited by 33
Variegated expression of the Sgs-4 locus in Drosophila melanogaster
Kornher, J. S., & Kauffman, S. A. (1986) · Chromosoma · doi:10.1007/bf00288495 · Cited by 25
Kraus, K. W., Lee, Y., Lis, J. T., & Wolfner, M. F. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.11.4756 · Cited by 19
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Peters, A. H. F. M., O'Carroll, D., Scherthan, H., Mechtler, K., Sauer, S., Schöfer, C., Weipoltshammer, K., Pagani, M., Lachner, M., Kohlmaier, A., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00542-6 · Cited by 1,766
Schotta, G., Ebert, A., Krauss, V., Fischer, A., Hoffmann, J., Rea, S., Jenuwein, T., Dorn, R., & Reuter, G. (2002) · The EMBO Journal · doi:10.1093/emboj/21.5.1121 · Cited by 714
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Rice, J. C., & Allis, C. D. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00208-8 · Cited by 701
Tschiersch, B., Hofmann, A., Krauss, V., Dorn, R., Korge, G., & Reuter, G. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06693.x · Cited by 567
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Metadata
- Authors
- Eissenberg, J. C., Morris, G. D., Reuter, G., & Hartnett, T.
- Affiliations
- Saint Louis University (US)
- ORCID
- Reuter, G
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-06-01
- Volume
- 131
- Issue
- 2
- Pages
- 345-352
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1856601345
- Cited by
- 315
- References
- 39
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 12 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 6 · 2019: 3 · 2018: 10 · 2017: 1 · 2016: 6 · 2015: 7 · 2014: 7 · 2013: 7 · 2012: 6
- FWCI
- 3.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01gm040732-02)
- National Institute of General Medical Sciences (GM40732)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Keywords
- heterochromatin, position effect variegation, dose-dependent effects, heterochromatin spreading, gene repression, Drosophila
- MeSH terms
- Chromobox Protein Homolog 5, Alleles, Animals, Chromosomal Proteins, Non-Histone, DNA, Drosophila melanogaster, Epistasis, Genetic, Gene Expression Regulation, Genes, Lethal, Heterochromatin, Mutation, Recombinant Fusion Proteins, Genes, Suppressor
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