Cited by 644Genomics and Chromatin DynamicsNuclear Structure and FunctionChromosomal and Genetic Variations

Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.

Open access
James, T C, Elgin, S C·1986·Molecular and Cellular Biology

Abstract

Monoclonal antibodies were prepared against a fraction of nuclear proteins of Drosophila melanogaster identified as tightly binding to DNA. Four of these antibodies were directed against a 19-kilodalton nuclear protein; immunofluorescence staining of the polytene chromosomes localized the antigen to the alpha, beta, and intercalary heterochromatic regions. Screening of a lambda gt11 cDNA expression library with one of the monoclonal antibodies identified a recombinant DNA phage clone that produced a fusion protein immunologically similar to the heterochromatin-associated protein. Polyclonal sera directed against the bacterial lacZ fusion protein recognized the same nuclear protein on Western blots. A full-length cDNA clone was isolated from a lambda gt10 library, and its DNA sequence was obtained. Analysis of the open reading frame revealed an 18,101-dalton protein encoded by this cDNA. Two overlapping genomic DNA clones were isolated from a Charon 4 library of D. melanogaster with the cDNA clone, and a restriction map was obtained. In situ hybridization with these probes indicated that the gene maps to a single chromosome location at 29A on the 2L chromosome. This general strategy should be effective for cloning the genes and identifying the genetic loci of chromosomal proteins which cannot be readily assayed by other means.

Integrity signals

Registry

Crossref · 10.1128/mcb.6.11.3862

Cites

58 of 58 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
James, T. C., & Elgin, S. C.
Venue
Molecular and Cellular Biology
Published
1986-11
Volume
6
Issue
11
Pages
3862-3872
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2101092089
Cited by
643
References
58
Citations by year
2026: 2 · 2025: 10 · 2024: 21 · 2023: 7 · 2022: 13 · 2021: 37 · 2020: 16 · 2019: 13 · 2018: 12 · 2017: 10 · 2016: 12 · 2015: 21 · 2014: 26 · 2013: 14 · 2012: 14
FWCI
2.74
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Cancer Society
  • National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
non-histone proteins, heterochromatin, Drosophila melanogaster, monoclonal antibodies, polytene chromosomes, cDNA cloning, gene mapping, chromosome 2L, chromatin proteins
MeSH terms
Amino Acid Sequence, Animals, Antibodies, Base Sequence, Chromosomal Proteins, Non-Histone, Chromosomes, DNA, Recombinant, Drosophila melanogaster, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Genes, Heterochromatin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review