The DOI matches its Crossref record; 49 of the 50 references it cites were found in a registry or an index.
Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
Abstract
Zinc-finger nucleases (ZFNs) are hybrids between a nonspecific DNA-cleavage domain and a DNA-binding domain composed of Cys2His2 zinc fingers. Because zinc fingers can be manipulated to recognize a broad range of sequences, these enzymes have the potential to direct cleavage to arbitrarily chosen targets. We have tested this idea by designing a pair of ZFNs that recognize a unique site in the yellow (y) gene of Drosophila. When these nucleases were expressed in developing larvae, they led to somatic mutations specifically in the y gene. These somatic mosaics were observed in approximately one-half of the males expressing both nucleases. Germline y mutations were recovered from 5.7% of males, but from none of the females, tested. DNA sequences were determined and showed that all of the mutations were small deletions and/or insertions located precisely at the designed target. These are exactly the types of alterations expected from nonhomologous end joining (NHEJ) following double-strand cleavage of the target. This approach promises to permit generation of directed mutations in many types of cells and organisms.
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In the citing paper
“In NHEJ, protein factors re-ligate the broken DNA strand either directly or by including nucleotide insertions or deletions (indels) (Hefferin & Tomkinson, 2005). This process occurs without a homologous DNA template, regularly leading to mutations and deletions in the repaired strand (Bibikova et al., 2002), as shown in Figure 1(A). NHEJ is, therefore, characterized as error-prone.”
From Delivering CRISPR: a review of the challenges and approaches (2018) · cites this paper as Bibikova et al., 2002
In the citing paper
“The design of a ZFN pair targeting opposite strands with an offset of 6 bp results in a DSB in the targeted DNA and can subsequently be repaired by NHEJ or HDR [88].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [88]
In the citing paper
“For example, if the nuclease action mutates unintended targets that are important for cellular physiology, the treated cells may become dysfunctional or even die [61,62,63].”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [61]
In the citing paper
“The fundamental safety concern for using ZFN mediated gene disruption for human therapy is the specificity of gene targeting.”
From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [61]
Citations
Cites
The Genome Sequence of Drosophila melanogaster
Adams, M. D., Celniker, S., Holt, R. A., Evans, C. A., Gocayne, J. D., Amanatides, P. G., Scherer, S. E., Li, P. W., Hoskins, R. A., Galle, R. F., et al. (2000) · Science · doi:10.1126/science.287.5461.2185 · Cited by 5,902
[12] One-step gene disruption in yeast
Rothstein, R. J. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)01015-0 · Cited by 3,282
Zinc Finger-DNA Recognition: Crystal Structure of a Zif268-DNA Complex at 2.1 Å
Pavletich, N. P., & Pabo, C. O. (1991) · Science · doi:10.1126/science.2028256 · Cited by 2,081
Altering the Genome by Homologous Recombination
Capecchi, M. R. (1989) · Science · doi:10.1126/science.2660260 · Cited by 2,037
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.
Kim, Y. G., Cha, J., & Chandrasegaran, S. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1156 · Cited by 1,889
Mansour, S. L., Thomas, K. R., & Capecchi, M. R. (1988) · Nature · doi:10.1038/336348a0 · Cited by 1,683
Chromosomal stability and the DNA double-stranded break connection
van Gent, D. C., Hoeijmakers, J. H. J., & Kanaar, R. (2001) · Nature Reviews Genetics · doi:10.1038/35056049 · Cited by 1,219
Design and Selection of Novel Cys2His2 Zinc Finger Proteins
Pabo, C. O., Peisach, E., & Grant, R. A. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.313 · Cited by 715
Stimulation of Homologous Recombination through Targeted Cleavage by Chimeric Nucleases
Bibikova, M. V., Carroll, D., Segal, D. J., Trautman, J. K., Smith, J., Kim, Y., & Chandrasegaran, S. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.1.289-297.2001 · Cited by 614
Self-citationGene Targeting by Homologous Recombination in Drosophila
Rong, Y. S., & Golic, K. G. (2000) · Science · doi:10.1126/science.288.5473.2013 · Cited by 557
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Sorted by citations
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Development and Applications of CRISPR-Cas9 for Genome Engineering
Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237
Metadata
- Authors
- Bibikova, M., Golic, M., Golic, K. G., & Carroll, D.
- Affiliations
- University of Utah (US)
- Corresponding author
- Carroll, Dana
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-07-01
- Volume
- 161
- Issue
- 3
- Pages
- 1169-1175
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1906155566
- Cited by
- 860
- References
- 50
- Citations by year
- 2026: 12 · 2025: 30 · 2024: 44 · 2023: 40 · 2022: 50 · 2021: 46 · 2020: 42 · 2019: 41 · 2018: 49 · 2017: 50 · 2016: 70 · 2015: 64 · 2014: 65 · 2013: 47 · 2012: 45
- FWCI
- 3.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (7r01gm060700-12)
- National Institute of General Medical Sciences (R01GM60700, R01 GM58504, R01GM058504)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- zinc finger nucleases, targeted mutagenesis, Drosophila, yellow gene, germline mutations, non-homologous end joining, double-strand DNA cleavage, C2H2 zinc finger, directed mutagenesis
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Crosses, Genetic, Deoxyribonucleases, DNA Transposable Elements, Drosophila, Female, Genetic Vectors, Male, Ribonucleases, Transformation, Genetic, Mutagenesis, Insertional, Zinc Fingers
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