Blood Consumption by the Cat Flea, Ctenocephalides felis (Siphonaptera: Pulicidae)
Abstract
The volume of blood consumed by actively reproducing female cat fleas, Ctenocephalides felis (Bouché), while feeding in confinement feeding chambers on cats for 3 h, was investigated using two radionuclide blood tags (51Cr-erythrocyte and 125I-albumin) and the gravimetric method. Female fleas consumed an average of 0.110 ml (+/- 0.026 ml) of blood per 100 females in 3 h as determined using the dual radionuclide system. The single 51Cr-erythrocyte tag overestimated blood consumption by 11.3% and the single 125I-albumin tag underestimated blood consumption by 6.4%. The gravimetric method underestimated blood consumption by 72.2% compared with the dual radionuclide value. Investigations of blood consumption of nonconfined female fleas were conducted with cats housed in metabolic cages and restricted from grooming. These investigations were conducted using the single 51Cr-erythrocyte tag, and the data obtained were corrected to compensate for using a single erythrocyte tag. Female cat fleas consumed an average of 13.6 microliters (+/- 2.7 microliters) of blood per day, which was equivalent to 15.15 times their body weight. In an additional study, direct exposure to 40 kHz ultrasound did not reduce blood consumption by the fleas.
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Blood-feeding adults of Ctenocephalides spp. may cause iron deficiency anaemia especially in puppies and kittens.
“Blood-feeding adults of Ctenocephalides spp. may cause iron deficiency anaemia especially in puppies and kittens [15,20].”
From Fleas infesting pets in the era of emerging extra-intestinal nematodes (2013) · cites this paper as [20]
In long-lasting infections, adult animals may suffer blood loss and chronic anaemia.
“In the case of long-lasting infections, also adult animals may suffer of blood loss and chronic anaemia [15,20].”
From Fleas infesting pets in the era of emerging extra-intestinal nematodes (2013) · cites this paper as [20]
Adult C. felis begins feeding almost immediately once on an animal, with many fleas feeding within minutes.
“Adult C. felis begins feeding almost immediately once on an animal, with many fleas feeding within minutes [20].”
From Fleas infesting pets in the era of emerging extra-intestinal nematodes (2013) · cites this paper as [20]
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Zellner, P. N., Njoroge, E., Lynch, A., et al. (2026) · Journal of Medical Entomology · doi:10.1093/jme/tjag037 · Cited by 2 · see citing works
Little, S. E. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-50934-3.00106-3 · Cited by 2 · see citing works
Koop, K., Kruis, S., Poelman, E., et al. (2013) · The Journal of Pediatrics · doi:10.1016/j.jpeds.2013.08.059 · Cited by 1 · see citing works
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Fellin, E., & Schulte‐Hostedde, A. I. (2021) · Parasitology · doi:10.1017/s0031182021001700 · Cited by 1 · see citing works
“, 2015), and can have severe negative effects on the physiology and fitness of their vertebrate hosts (Dryden and Gaafar, 1991; Carleton, 2008; Pfäffle et al., 2009; Bordes et al., 2007; Godinho et al., 2013; Hersh et al., 2014; Jones et al., 2019).”
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Mackensen, H. (2006) · Elektronische Hochschulschriften der LMU München (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.5847 · Cited by 1 · see citing works
Bi‐song, W., Fang-dong, Z., Qi‐zhi, S., et al. (2002) · Insect Science · doi:10.1111/j.1744-7917.2002.tb00467.x · Cited by 1 · see citing works
Seguela, M. J.-P., Franc, M., Bret-Bennis, M. L., & Bonneau, M. S. (2007)
Bartonella and other plagues - New insights on fleas
Krämer, F. (2009)
Epidemiologische Untersuchungen zum Flohbefall bei Hunden und Katzen im Raum Leipzig
Pfister, K., & Beck, W. (2008)
against induced infestations of Ctenocephalides felis on dogs
Six, R. H., Liebenberg, J. E., Honsberger, N. A., & Mahabir, S. P. (2016)
Bowman, D. D. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-54396-5.00011-8
Englar, D. R. E. (2019) · Wiley eBooks · doi:10.1002/9781119414612.ch6
(2004) · CRC Press eBooks · doi:10.1201/9780203461730.ch6
(2019) · Elsevier eBooks · doi:10.1016/b978-0-12-813890-8.00003-1
Pesenato, I. P. (2023) · doi:10.11606/t.10.2023.tde-22022024-104748
(2000) · Time to knit · doi:10.1016/s1283-0828(05)42128-6
Miranda, F. R., Bonfim, I. V., Fernandes, A. J. B., et al. (2026) · Veterinary Parasitology · doi:10.1016/j.vetpar.2026.110793
Rodrigues, P. H. C., Filho, A. A. P., Alves, J. P. S., et al. (2026) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2026.104613
Lynch, A., Mauricio, M., Palomino, Y., et al. (2026) · Insects · doi:10.3390/insects17090952
“Adult C. felis begin feeding immediately after acquiring a host, mate shortly thereafter, and females become reproductively active within the first few days [48,49].”
- Authors
- Dryden, M. W., & Gaafar, S. M.
- Affiliations
- Purdue University West Lafayette (US)
- Venue
- Journal of Medical Entomology
- Publisher
- Oxford University Press (OUP)
- Published
- 1991-05-01
- Volume
- 28
- Issue
- 3
- Pages
- 394-400
- ISSN
- 0022-2585 (ISSN-L)
- 1938-2928
- OpenAlex
- W2095540646
- Cited by
- 66
- References
- 0
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 2 · 2023: 3 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 7 · 2015: 1 · 2014: 3 · 2013: 5 · 2012: 2
- FWCI
- 0.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medical Entomology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Yersinia bacterium, plague, ectoparasites research
- Health Sciences › Medicine › Infectious Diseases › Dermatological diseases and infestations
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Keywords
- Ctenocephalides felis, blood consumption, feeding behavior, 125I-albumin, gravimetric method, ultrasound exposure
- MeSH terms
- Animals, Cats, Feeding Behavior, Female, Siphonaptera, Male
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