The DOI matches its Crossref record; 61 of the 63 references it cites were found in a registry or an index.
A Vegetable/Fruit Concentrate with High Antioxidant Capacity Has No Effect on Biomarkers of Antioxidant Status in Male Smokers
Open accessAbstract
The potential benefits of a high fruit and vegetable intake on the antioxidant status and on relevant biomarkers of oxidative damage to lipids, proteins and DNA and on (functional) markers of oxidative stress were evaluated. A randomized, free living, open placebo-controlled cross-over trial of 3 wk, with a 2-wk washout period between treatments, was performed in a group of 22 male smokers with a relatively low vegetable and fruit intake using a vegetable burger and fruit drink. The vegetable burger and fruit drink increased serum levels of vitamin C, alpha-carotene, beta-carotene, beta-cryptoxanthin and zeaxanthin and plasma total antioxidant capacity. However, no effects were demonstrated on any marker of oxidative damage to lipids (malondialdehyde F(2)-isoprostane) proteins (carbonyls) and DNA (Comet assay) and (functional) markers of oxidative stress (reduced/oxidized glutathione ratio, glutathione-S-transferase alpha, glutathione-S-transferase pi and nuclear transcription factor-kappaB). Apparently, these increased levels of antioxidants in serum were not sufficiently high to show beneficial changes with the selected biomarkers. Alternatively, oxidative stress in male smokers with a relatively low fruit and vegetable intake might have been still too low to demonstrate a beneficial effect of antioxidants.
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“Benefits were seen in healthy subjects and in those with existing metabolic risk factors.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [36]
In the citing paper
“Human intervention studies using chokeberries, cranberries, blueberries, and strawberries (either fresh, or as juice, or freeze-dried), or purified anthocyanin extracts have demonstrated significant improvements in LDL oxidation, lipid peroxidation, total plasma antioxidant capacity, dyslipidemia, and glucose metabolism.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [36]
In the citing paper
“The most significant outcomes of these clinical studies show an increase in plasma or urinary antioxidant capacity, a decrease in LDL oxidation and lipid peroxidation, a decrease in plasma glucose or total cholesterol, and an increase in HDL-cholesterol following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [36]
In the citing paper
“Berries were also shown to increase plasma antioxidant capacity36 and to decrease lipid peroxidation42 in smokers who are at high risk of developing CVD.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [36]
In the citing paper
“Clinical studies in healthy humans, subjects with diabetes mellitus, dyslipidemia, metabolic syndrome, hypertension, or in smokers, show a significant decrease in CVD risk factors, especially glucose, lipids and lipid peroxidation, and systolic blood pressure, following berry intervention.”
From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [36]
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Cites
Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972) · Clinical Chemistry · doi:10.1093/clinchem/18.6.499 · Cited by 32,423
Hayes, J. D., & Pulford, D. J. (1995) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239509083491 · Cited by 3,464
Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence.
Block, G., Patterson, B. H., & Subar, A. (1992) · Nutrition and Cancer · doi:10.1080/01635589209514201 · Cited by 3,321
Role of oxidative stress in diabetic complications: a new perspective on an old paradigm.
Baynes, J. W., & Thorpe, S. R. (1999) · Diabetes · doi:10.2337/diabetes.48.1.1 · Cited by 2,441
Antioxidant and redox regulation of gene transcription
Sen, C. K., & Packer, L. (1996) · The FASEB Journal · doi:10.1096/fasebj.10.7.8635688 · Cited by 1,972
Tomatoes, Tomato-Based Products, Lycopene, and Cancer: Review of the Epidemiologic Literature
Giovannucci, E. L. (1999) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/91.4.317 · Cited by 1,485
Morrow, J. D., Frei, B. B., Longmire, A. W., Gaziano, J. M., Lynch, S. M., Shyr, Y., Strauss, W., Oates, J. A., & Roberts, L. J. (1995) · New England Journal of Medicine · doi:10.1056/nejm199505043321804 · Cited by 1,340
Omenn, G. S., Goodman, G. E., Thornquist, M. D., Balmes, J., Cullen, M. R., Glass, A., Keogh, J. P., Meyskens, F. L., Valanis, B., Williams, J. H., et al. (1996) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/88.21.1550 · Cited by 1,255
Cancer risk and oxidative DNA damage in man
Loft, S. H., & Poulsen, H. E. (1996) · Journal of Molecular Medicine · doi:10.1007/bf00207507 · Cited by 964
Carr, A. C., & Frei, B. (1999) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/69.6.1086 · Cited by 940
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2 of the 63 references couldn’t be matched to a known paper.
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- Hulshof, K.F.A.M., Kistenmaker, C., Bouwmans, M. & Löwik, M.R.H. (1998) Energy and nutrient intake of the Dutch population—Dutch Nutrition Surveillance System 1997–1998. TNO report V98.805 (in Dutch), pp. 1–13.
- Van den Berg, 2000, The potential for the improvement of carotenoid levels in food and the likely systemic effects, J. Sci. Food Agric, 80, 880, 10.1002/(SICI)1097-0010(20000515)80:7<880::AID-JSFA646>3.0.CO;2-1 · DOI
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Sorted by citations
Items 101–143 of 143
Kim, H.-Y., Kim, J., Lee, S.-A., Chang, H.-E., Park, M.-H., Hwang, S., Lee, J.-Y., Mok, C., & Hong, S.-G. (2008) · Journal of Medicinal Food · doi:10.1089/jmf.2007.549 · Cited by 12
Ibrahim, M., Ismail, A., Al‐Sheraji, S. H., Azlan, A., & Hamid, A. A. (2013) · Journal of Functional Foods · doi:10.1016/j.jff.2013.09.011 · Cited by 9
Biochemical Measures of Antioxidant Status
Prior, R. L. (2004) · Topics in Clinical Nutrition · doi:10.1097/00008486-200407000-00007 · Cited by 9
Biomarkers of oxidative stress in overweight men are not influenced by a combination of antioxidants
Rytter, E., Johansson, C., Vessby, B. O., Sjödin, A. M., Möller, L., Åkesson, B., & Basu, S. (2010) · Free Radical Research · doi:10.3109/10715761003647101 · Cited by 8
“The 6-a-day study from Denmark, where healthy subjects consumed daily six servings of fruit and vegetables [41] and another study supplementing male smokers with a vegetable burger and a fruit drink rich in antioxidants [42], did not alter the levels of 8-iso-PGF 2α, while 500 ml high-pressurized orange juice reduced…”
MethodFruit and vegetables, cardiovascular disease, diabetes and obesity
Amiot, M. J., & Lairon, D. (2008) · Elsevier eBooks · doi:10.1533/9781845694289.2.95 · Cited by 9
Plant Polyphenol Intake Alters Gene Expression in Canine Leukocytes
Salas, A., Subirada, F., Miguel Pérez-enciso, A., Blanch, F., Jeusette, I. C., Romano, V., & Torre, C. (2009) · Lifestyle Genomics · doi:10.1159/000200018 · Cited by 7
The Biochemistry Behind the Potential Cardiovascular Protection by Dietary Flavonoids
Loke, W. M., Hodgson, J. M., & Croft, K. D. (2009) · doi:10.1002/9780470531792.ch5 · Cited by 7
Dallinga, J. W., Haenen, G. R. M. M., Bast, A., & van Schooten, F. (2002) · Biomarkers · doi:10.1080/13547500210148306 · Cited by 6
“Recently it has been shown that nuclear factor-µB, an oxidative stress-sensitive transcription factor, is higher in the peripheral blood mononuclear cells of smokers compared with non-smokers (Van den Berg et al. 2001a).”
BackgroundBhatnagar, D. (2002) · Current Opinion in Lipidology · doi:10.1097/00041433-200202000-00012 · Cited by 6
Vauzour, D., Vafeiadou, K., & Spencer, J. P. E. (2012) · Phytonutrients · doi:10.1002/9781118253649.ch4 · Cited by 5
International Validation of the Comet Assay and a Human Intervention Study.
Ersson, C. (2011) · Cited by 4
Quiroga, A., Colussi, C., Odetti, L. M., Loteste, A., Paonessa, A. M., Mastandrea, C. R., Grigolato, R., Poletta, G. L., Sigrist, M. E., & Simoniello, M. F. (2024) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2024.503787 · Cited by 3
Comet Assays in Dietary Intervention Trials
Nersesyan, A. K., Hoelzl, C., Ferk, F., Mišík, M., & Knasmüller, S. (2009) · Royal Society of Chemistry eBooks · doi:10.1039/9781847559746-00267 · Cited by 3
Use of Single-cell Gel Electrophoresis Assays in Dietary Intervention Trials
Nersesyan, A. K., Hoelzl, C., Ferk, F., Mišík, M., Al-Serori, H., Setayesh, T., & Knasmüller, S. (2016) · Royal Society of Chemistry eBooks · doi:10.1039/9781782622895-00314 · Cited by 3
Possible action of grape seed oil on brain toxicity induced by methomyl or imidacloprid of male rats
Moeen, D., Amer, A., Ismail, N., & Ali, E. H. A. (2018) · Journal of Scientific Research in Science /Journal of Scientific Research in Science · doi:10.21608/jsrs.2018.25535 · Cited by 3
“…authors found no effect on the activity of erythrocyte antioxidant enzymes in human intervention studies after administration of diets enriched in PP from red wine, beer and spirits (Van der Gaag et al., 2000) from vegetable and fruit (Van der Berg et al., 2001) or green tea (Young et al., 2002).”
BackgroundLorenzoni, G., Minto, C., Vecchio, M. G., Zec, S., Paolin, I., Lamprecht, M., Mestroni, L., & Gregori, D. (2019) · Journal of Clinical Medicine · doi:10.3390/jcm8111914 · Cited by 4
“Two studies associated the fruit and/or vegetable concentrate to a specific diet: van den Berg includes one controlled meal with a vegetable burger, while patients enrolled by Antal had a low-fattydiet prescription [22,51].”
InfluentialAjuwon, O. R., Oguntibeju, O. O., & Marnewick, J. L. (2016) · African Journal of Traditional Complementary and Alternative Medicines · doi:10.21010/ajtcam.v13i3.1 · Cited by 2
Wisnuwardani, R. W., Henauw, S. D., Forsner, M., Gottrand, F., Huybrechts, I., Kafatos, A., Kersting, M., Knaze, V., Μanios, Y., Nova, E., et al. (2021) · International Journal of Food Sciences and Nutrition · doi:10.1080/09637486.2021.1910631 · Cited by 2
“…contributor of hesperidin, higher consumption of fruits and vegetables (Wisnuwardani et al. 2019) was found for greater TAC from diet and supplementation in U.S. adults (Kim et al. 2016), but fruit and vegetables consumption did not affect plasma TAC (Record et al. 2001; van den Berg et al. 2001).”
BackgroundHealth effects of quercetin : from mechanism to nutraceutical
Boots, A. W. (2006) · doi:10.26481/dis.20061221ab · Cited by 2
Shahin, A., Ross, A. B., Turner, R. M., Mann, J. I., & Reynolds, A. N. (2025) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.70334 · Cited by 1
Role of the Antioxidant Network in the Prevention of Age-Related Diseases
Serafini, M. (2008) · Humana Press eBooks · doi:10.1007/978-1-59745-420-9_15 · Cited by 1
“In healthy smoking subjects, plasma TAC increased after consumption of five portions of fruits and vegetables per day for 3 weeks and after consumption of vegetable burger plus a fruit drink for 3 weeks [50, 51].”
BackgroundAjuwon, O. R., Oguntibeju, O. O., & Marnewick, J. L. (2016) · African Journal of Traditional Complementary and Alternative Medicines · doi:10.4314/ajtcam.v13i3.1 · Cited by 1
The Role of Antioxidants in the Prevention of Oxidative Damage to Nucleic Acids
Möller, P., & Loft, S. H. (2007) · Springer eBooks · doi:10.1007/978-0-387-72974-9_16 · Cited by 1
Qualidade sanitária e nutricional de hortícolas organicas
Arbos, K. A. (2009) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) · Cited by 1
Dugo, L., Neğiş, Y., & Azzi, A. (2011) · Encyclopedia of Life Sciences · doi:10.1002/9780470015902.a0023204 · Cited by 1
Measurement and evaluation of antioxidant status and relation to oxidative stress in humans.
Nälsén, C. (2006) · KTH Publication Database DiVA (KTH Royal Institute of Technology) · Cited by 1
Modulation of Cigarette Smoke Effects by Antioxidants: Oxidative Stress and Degenerative Diseases
Kaikkonen, J., & Salonen, J. T. (2007) · Springer eBooks · doi:10.1007/3-540-32232-9_8
Antioxidant activity: from model to man
Balk, J. M. (2010) · doi:10.26481/dis.20100520jb
Baktiyani, S. C. W. (2013) · Jurnal Kedokteran Brawijaya · doi:10.21776/ub.jkb.2007.023.03.6
Oxidative Stress in Breast Cancer Carcinogenesis
Martin, L. J., & Boyd, N. F. (2009) · doi:10.1002/9783527628100.ch26
NEW METHODS FOR ASSESSMENT OF NUTRITIONAL AND HEALTH EFFECTS OF CEREAL PRODUCTS
van der Kamp, J. W., van den Berg, R., Venema, K., & Havenaar, R. (2005) · Elsevier eBooks · doi:10.1533/9781845690632.4.118
Pincemail, Paquot, N., Cillard, J., Hininger‐Favier, I., Iuliano, L., Cazaubiel, M., Guéraud, F., Chapelle, J.-P., Kevers, C., Charlier, C., et al. (2013) · Journal of Pharmacy and Nutrition Sciences · doi:10.6000/1927-5951.2013.03.03.3
“Globally, our findings are in agreement with those of previous studies indicating that a decrease in the biomarkers of oxidative stress did not occur after increased fruits and vegetables intake [16-24,49,50] despite or not an improvement of the plasma antioxidant status.”
ResultTHE EFFECT OF ENCAPSULATION ON THE IN VITRO ANTI-OXIDATIVE ACTIVITY OF RUTIN
Jantrawut, P. (2014)
The effect of Protandim supplementation on oxidative damage and athletic performance.
Ueberschlag, S. L. (2015) · doi:10.18297/etd/2209
DANOS OXIDATIVOS NO DNA EM LINFÓCITOS DE SANGUE PERIFÉRICO
Filho, M. (2009)
Antioxidative Nutrients and Oxidative DNA Damage
Umegaki, K. (2004) · The Japanese Journal of Nutrition and Dietetics · doi:10.5264/eiyogakuzashi.62.65
UNIVERSIDAD NACIONAL DE CÓRDOBA FACULTAD DE CIENCIAS MÉDICAS
Barrionuevo, O. T., & Argentina, R. (2012)
Clinical evidence for biological activity of fruit and vegetable phytochemicals
Johnson, I. T. (2008) · Elsevier eBooks · doi:10.1533/9781845694289.3.279 · Cited by 1
DNA Strand Breaks and Tomatoes
Briviba, K. (2008) · CRC Press eBooks · doi:10.1201/9781439843390-19
DNA Strand Breaks and Tomatoes
Briviba, K. (2008) · Science Publishers eBooks · doi:10.1201/9781439843390-c19
(2005) · Wiley eBooks · doi:10.1002/9780470774663.refs
Prado, R. P. (2009) · Americanae (AECID Library)
Metadata
- Authors
- van den Berg, R., van Vliet, T., Broekmans, W. M. R., Cnubben, N. H. P., Vaes, W. H. J., Roza, L., Haenen, G. R. M. M., Bast, A., & van den Berg, H.
- Affiliations
- Maastricht University (NL)
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2001-06
- Volume
- 131
- Issue
- 6
- Pages
- 1714-1722
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2099422363
- Cited by
- 144
- References
- 63
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 3 · 2019: 3 · 2018: 4 · 2016: 3 · 2015: 4 · 2014: 5 · 2013: 10 · 2012: 8
- FWCI
- 6.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, other-oa)
- TNO Repository (submittedVersion)
- Repository hosted by TU Delft Library (TU Delft) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Keywords
- antioxidant status, oxidative stress, smokers, lipid peroxidation, protein carbonylation, DNA damage, glutathione, glutathione S-transferase, NF-κB, carotenoids, vitamin C, dietary supplementation
- MeSH terms
- Adult, Antioxidants, Blood Pressure, Body Weight, Carotenoids, Cholesterol, DNA Damage, Fruit, Humans, Cholesterol, HDL, Male, Malondialdehyde, Middle Aged, Monocytes, Smoking, Time Factors, Triglycerides, Vegetables, Vitamin A, Vitamin E, Oxidative Stress, Cross-Over Studies
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