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Cited by 447Protein Kinase Regulation and GTPase SignalingGenomics and Phylogenetic StudiesCellular transport and secretion

Evolution of the Rho Family of Ras-Like GTPases in Eukaryotes

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Boureux, Anthony, Vignal, Emmanuel, Faure, Sandrine, Fort, Philippe·2007·Molecular Biology and Evolution
DOI 10.1093/molbev/msl145PMC PMC2665304
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Abstract

GTPases of the Rho family are molecular switches that play important roles in converting and amplifying external signals into cellular effects. Originally demonstrated to control the dynamics of the F-actin cytoskeleton, Rho GTPases have been implicated in many basic cellular processes that influence cell proliferation, differentiation, motility, adhesion, survival, or secretion. To elucidate the evolutionary history of the Rho family, we have analyzed over 20 species covering major eukaryotic clades from unicellular organisms to mammals, including platypus and opossum, and have reconstructed the ontogeny and the chronology of emergence of the different subfamilies. Our data establish that the 20 mammalian Rho members are structured into 8 subfamilies, among which Rac is the founder of the whole family. Rho, Cdc42, RhoUV, and RhoBTB subfamilies appeared before Coelomates and RhoJQ, Cdc42 isoforms, RhoDF, and Rnd emerged in chordates. In vertebrates, gene duplications and retrotranspositions increased the size of each chordate Rho subfamily, whereas RhoH, the last subfamily, arose probably by horizontal gene transfer. Rac1b, a Rac1 isoform generated by alternative splicing, emerged in amniotes, and RhoD, only in therians. Analysis of Rho mRNA expression patterns in mouse tissues shows that recent subfamilies have tissue-specific and low-level expression that supports their implication only in narrow time windows or in differentiated metabolic functions. These findings give a comprehensive view of the evolutionary canvas of the Rho family and provide guides for future structure and evolution studies of other components of Rho signaling pathways, in particular regulators of the RhoGEF family.

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Authors
Boureux, A., Vignal, E., Faure, S., & Fort, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Physiologie et Médecine Expérimentale du Coeur et des Muscles (FR)
ORCID
Venue
Molecular Biology and Evolution
Publisher
Oxford University Press (OUP)
Published
2006-10-11
Volume
24
Issue
1
Pages
203-216
ISSN
  • 0737-4038 (ISSN-L)
  • 1537-1719
DOI
10.1093/molbev/msl145
PMC
PMC2665304
OpenAlex
W1966201817
Cited by
447
References
2
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FWCI
5.79
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology and Evolution (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • HAL (submittedVersion, other-oa)
  • HAL (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
Rho GTPases, Ras-related GTPases, molecular switches, Rac, Cdc42, RhoH, RhoD, gene duplication, retrotransposition, horizontal gene transfer, alternative splicing, chordate evolution, eukaryotic evolution, Rho signaling pathway, tissue-specific gene expression, actin cytoskeleton
MeSH terms
Amino Acid Sequence, Animals, Fungi, Humans, Invertebrates, Molecular Sequence Data, Phylogeny, Plants, Pseudogenes, Vertebrates, Sequence Alignment, Evolution, Molecular, Gene Duplication, rho GTP-Binding Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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