The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs
Open accessAbstract
Intercellular exchange of protein and RNA-containing microparticles is an increasingly important mode of cell-cell communication. Here we investigate if mesenchymal stem cells (MSCs) known for secreting therapeutic paracrine factors also secrete RNA-containing microparticles. We observed that human embryonic stem cell (hESC)-derived MSC conditioned medium contained small RNAs (less than 300 nt) encapsulated in cholesterol-rich phospholipid vesicles as evidenced by their RNase sensitivity only in the presence of a sodium dodecyl sulfate-based cell lysis buffer, phospholipase A2 and a chelator of cholesterol, cyclodextrin and the restoration of their lower than expected density by detergent or phospholipase A2 treatment. MicroRNAs (miRNAs) such as hsa-let-7b and hsa-let-7g were present in a high precursor (pre)- to mature miRNA ratio by microarray analysis and quantitative reverse transcription-polymerase chain reaction. The pre-miRNAs were cleaved to mature miRNA by RNase III in vitro. High performance liquid chromatography-purified RNA-containing vesicles have a hydrodynamic radius of 55-65 nm and were readily taken up by H9C2 cardiomyocytes. This study suggests that MSCs could facilitate miRNA-mediated intercellular communication by secreting microparticles enriched for pre-miRNA.
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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
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B lymphocytes secrete antigen-presenting vesicles.
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Cited by
Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Biological properties of extracellular vesicles and their physiological functions
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Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury
Lai, R. C., Arslan, F., Lee, M. M., Sze, N. S. K., Choo, A., Chen, T. S., Salto-Tellez, M., Timmers, L., Lee, C. N., El Oakley, R. M., et al. (2010) · Stem Cell Research · doi:10.1016/j.scr.2009.12.003 · Cited by 2,215
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Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy
Lee, Y., EL Andaloussi, S., & Wood, M. J. A. (2012) · Human Molecular Genetics · doi:10.1093/hmg/dds317 · Cited by 938
Metadata
- Authors
- Chen, T. S., Lai, R. C., Lee, M. M., Choo, A. B. H., Lee, C. N., & Lim, S. K.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-10-22
- Volume
- 38
- Issue
- 1
- Pages
- 215-224
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2119376007
- Cited by
- 658
- References
- 6
- FWCI
- 10.32
- Type
- journal article
- Language
- en
- Open access
- gold
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