The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
Open accessAbstract
The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.
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Citations
Cites
Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1
Tahiliani, M., Koh, K. P., Shen, Y., Pastor, W. A., Bandukwala, H., Brudno, Y., Agarwal, S., Iyer, L. M., Liu, D. R., Aravind, L., et al. (2009) · Science · doi:10.1126/science.1170116 · Cited by 5,836
The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain
Kriaucionis, S., & Heintz, N. (2009) · Science · doi:10.1126/science.1169786 · Cited by 2,700
Dnmt1 and Dnmt3a maintain DNA methylation and regulate synaptic function in adult forebrain neurons
Feng, J., Zhou, Y., Campbell, S. L., Le, T., Li, E., Sweatt, J. D., Silva, A. J., & Fan, G. (2010) · Nature Neuroscience · doi:10.1038/nn.2514 · Cited by 1,019
The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing
Huang, Y., Pastor, W. A., Shen, Y., Tahiliani, M., Liu, D. R., & Rao, A. (2010) · PLoS ONE · doi:10.1371/journal.pone.0008888 · Cited by 717
Acquired mutations in TET2 are common in myelodysplastic syndromes
Langemeijer, S. M. C., Kuiper, R. P., Berends, M., Knops, R., Aslanyan, M. G., Massop, M., Stevens-Linders, E., van Hoogen, P., van Kessel, A. G., Raymakers, R. A. P., et al. (2009) · Nature Genetics · doi:10.1038/ng.391 · Cited by 680
Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., Shimotohno, K., Ishikawa, F., Li, E., Ueda, H. R., et al. (2006) · Genes to Cells · doi:10.1111/j.1365-2443.2006.00984.x · Cited by 542
Lorsbach, R. B., Moore, J., Mathew, S., Raimondi, S. C., Mukatira, S. T., & Downing, J. R. (2003) · Leukemia · doi:10.1038/sj.leu.2402834 · Cited by 416
Iyer, L. M., Tahiliani, M., Rao, A., & Aravind, L. (2009) · Cell Cycle · doi:10.4161/cc.8.11.8580 · Cited by 397
The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid
Penn, N. W., Suwalski, R., O'Riley, C., Bojanowski, K., & Yura, R. (1972) · Biochemical Journal · doi:10.1042/bj1260781 · Cited by 280
Ono, R., Taki, T., Taketani, T., Taniwaki, M., Kobayashi, H., & Hayashi, Y. (2002) · PubMed · Cited by 278
Cited by
DNA methylation and its basic function
Moore, L. D., Le, T., & Fan, G. (2013) · Neuropsychopharmacology · doi:10.1038/npp.2012.112 · Cited by 4,906
Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine
Song, C.-X., Szulwach, K. E., Fu, Y., Dai, Q., Yi, C., Li, X., Li, Y., Chen, C.-H., Zhang, W., Jian, X., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1732 · Cited by 1,085
Role of TET enzymes in DNA methylation, development, and cancer
Rasmussen, K. D., & Helin, K. (2016) · Genes & Development · doi:10.1101/gad.276568.115 · Cited by 1,040
“By comparison, the products of further TET-mediated oxidation, 5fC and 5caC, are observed at ∼10-fold to 100-fold lower levels than 5hmC and often close to or under the detection limit (Kriaucionis and Heintz 2009; Globisch et al. 2010; Szwagierczak et al. 2010; He et al. 2011; Ito et al. 2011; Pfaffeneder et al. 2011…”
BackgroundReversing DNA Methylation: Mechanisms, Genomics, and Biological Functions
Wu, H., & Zhang, Y. (2014) · Cell · doi:10.1016/j.cell.2013.12.019 · Cited by 1,065
“…Chemical Labeling 5hmCwith Biotin (hMe-Seal or GLIB) By exploiting the naturally occurring glucosylation process of 5hmC by b-GT, several methods were developed to chemically and enzymatically label 5hmC with the biotin tag (Pastor et al., 2011; Song et al., 2011; Szwagierczak et al., 2010).”
InfluentialTET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity
Williams, K., Christensen, J., Pedersen, M. T., Johansen, J. V., Cloos, P. A. C., Rappsilber, J., & Helin, K. (2011) · Nature · doi:10.1038/nature10066 · Cited by 1,021
Metadata
- Authors
- Szwagierczak, A., Bultmann, S., Schmidt, C. S., Spada, F., & Leonhardt, H.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-08-04
- Volume
- 38
- Issue
- 19
- Pages
- e181-e181
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2143798330
- Cited by
- 432
- References
- 15
- FWCI
- 17.37
- Type
- journal article
- Language
- en
- Open access
- gold
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- Szwagierczak, A., Bultmann, S., Schmidt, C. S., Spada, F., & Leonhardt, H. (2010). Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucleic Acids Research. https://doi.org/10.1093/nar/gkq684
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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