The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
SLiCE: a novel bacterial cell extract-based DNA cloning method
Open accessAbstract
We describe a novel cloning method termed SLiCE (Seamless Ligation Cloning Extract) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (≥15 bp) with or without flanking heterologous sequences and provides an effective strategy for directional subcloning of DNA fragments from Bacteria Artificial Chromosomes (BACs) or other sources. SLiCE is highly cost effective as a number of standard laboratory bacterial strains can serve as sources for SLiCE extract. In addition, the cloning efficiencies and capabilities of these strains can be greatly improved by simple genetic modifications. As an example, we modified the DH10B Escherichia coli strain to express an optimized λ prophage Red recombination system. This strain, termed PPY, facilitates SLiCE with very high efficiencies and demonstrates the versatility of the method.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.14.4121-4130.1995 · Cited by 4,935
Construction of Biologically Functional Bacterial Plasmids In Vitro
Cohen, S. N., Chang, A. C. Y., Boyer, H. W., & Helling, R. B. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.11.3240 · Cited by 1,802
An efficient recombination system for chromosome engineering in Escherichia coli
Yu, D., Ellis, H. M., Lee, E.-C., Jenkins, N. A., Copeland, N. G., & Court, D. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.100127597 · Cited by 1,688
Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome
Gibson, D. G., Benders, G. A., Andrews-Pfannkoch, C., Denisova, E. A., Baden-Tillson, H., Zaveri, J., Stockwell, T. B., Brownley, A., Thomas, D. W., Algire, M. A., et al. (2008) · Science · doi:10.1126/science.1151721 · Cited by 1,243
A new logic for DNA engineering using recombination in Escherichia coli
Zhang, Y., Buchholz, F., Muyrers, J. P. P., & Stewart, A. F. (1998) · Nature Genetics · doi:10.1038/2417 · Cited by 1,222
Self-citationLigation-independent cloning of PCR products (LIC-PCR)
Aslanidis, C., & de Jong, P. J. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.20.6069 · Cited by 1,204
Lee, E.-C., Yu, D., Martinez de Velasco, J., Tessarollo, L., Swing, D. A., Court, D. L., Jenkins, N. A., & Copeland, N. G. (2001) · Genomics · doi:10.1006/geno.2000.6451 · Cited by 1,178
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
Li, M. Z., & Elledge, S. J. (2007) · Nature Methods · doi:10.1038/nmeth1010 · Cited by 953
Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ
Kuzminov, A. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.4.751-813.1999 · Cited by 896
A Restriction enzyme from Hemophilus influenzae
Smith, H. O., & Welcox, K. W. (1970) · Journal of Molecular Biology · doi:10.1016/0022-2836(70)90149-x · Cited by 884
Cited by
Large-scale de novo DNA synthesis: technologies and applications
Kosuri, S., & Church, G. M. (2014) · Nature Methods · doi:10.1038/nmeth.2918 · Cited by 869
Synthetic biology to access and expand nature's chemical diversity
Smanski, M. J., Zhou, H., Claesen, J., Shen, B., Fischbach, M. A., & Voigt, C. A. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2015.24 · Cited by 481
Genome‐scale engineering for systems and synthetic biology
Esvelt, K. M., & Wang, H. H. (2013) · Molecular Systems Biology · doi:10.1038/msb.2012.66 · Cited by 320
Xia, Y., Li, K., Li, J., Wang, T., Gu, L., & Xun, L. (2019) · Nucleic Acids Research · doi:10.1093/nar/gky1169 · Cited by 317
Global spread of three multidrug-resistant lineages of Staphylococcus epidermidis
Lee, J. Y. H., Monk, I. R., Gonçalves da Silva, A., Seemann, T., Chua, K. Y. L., Kearns, A., Hill, R., Woodford, N., Bartels, M. D., Strommenger, B., et al. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0230-7 · Cited by 299
Metadata
- Authors
- Zhang, Y., Werling, U., & Edelmann, W.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-01-11
- Volume
- 40
- Issue
- 8
- Pages
- e55-e55
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2078290510
- Cited by
- 493
- References
- 22
- FWCI
- 9.87
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: SLiCE: a novel bacterial cell extract-based DNA cloning method. https://app.cochat.ai/papers/10.1093/nar/gkr1288
- The paper
- Zhang, Y., Werling, U., & Edelmann, W. (2012). SLiCE: a novel bacterial cell extract-based DNA cloning method. Nucleic Acids Research. https://doi.org/10.1093/nar/gkr1288
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
