The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks
Open accessAbstract
After the generation of DNA double-strand breaks (DSBs), poly(ADP-ribose) polymerase-1 (PARP-1) is one of the first proteins to be recruited and activated through its binding to the free DNA ends. Upon activation, PARP-1 uses NAD+ to generate large amounts of poly(ADP-ribose) (PAR), which facilitates the recruitment of DNA repair factors. Here, we identify the RNA-binding protein NONO, a partner protein of SFPQ, as a novel PAR-binding protein. The protein motif being primarily responsible for PAR-binding is the RNA recognition motif 1 (RRM1), which is also crucial for RNA-binding, highlighting a competition between RNA and PAR as they share the same binding site. Strikingly, the in vivo recruitment of NONO to DNA damage sites completely depends on PAR, generated by activated PARP-1. Furthermore, we show that upon PAR-dependent recruitment, NONO stimulates nonhomologous end joining (NHEJ) and represses homologous recombination (HR) in vivo. Our results therefore place NONO after PARP activation in the context of DNA DSB repair pathway decision. Understanding the mechanism of action of proteins that act in the same pathway as PARP-1 is crucial to shed more light onto the effect of interference on PAR-mediated pathways with PARP inhibitors, which have already reached phase III clinical trials but are until date poorly understood.
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Cites
The DNA Damage Response: Making It Safe to Play with Knives
Ciccia, A., & Elledge, S. J. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.09.019 · Cited by 4,368
The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway
Lieber, M. R. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.093131 · Cited by 2,882
ATM-Dependent Chromatin Changes Silence Transcription In cis to DNA Double-Strand Breaks
Shanbhag, N. M., Rafalska-Metcalf, I. U., Balane-Bolivar, C., Janicki, S. M., & Greenberg, R. A. (2010) · Cell · doi:10.1016/j.cell.2010.04.038 · Cited by 737
Chou, D. M., Adamson, B., Dephoure, N. E., Tan, X., Nottke, A. C., Hurov, K. E., Gygi, S. P., Colaiácovo, M. P., & Elledge, S. J. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1012946107 · Cited by 529 · cited 1×
“To date, many proteins have been shown to be recruited in a PAR-dependent manner by cell imaging techniques: MRE11 (6), NBS1 (6), APLF (46), XRCC1 (57), CHD4 (58), NuRD (59) and ALC1 (47).”
BackgroundAdamson, B., Smogorzewska, A., Sigoillot, F. D., King, R. W., & Elledge, S. J. (2012) · Nature Cell Biology · doi:10.1038/ncb2426 · Cited by 433
Polo, S. E., Kaidi, A., Baskcomb, L., Galanty, Y., & Jackson, S. P. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.188 · Cited by 374 · cited 1×
“To date, many proteins have been shown to be recruited in a PAR-dependent manner by cell imaging techniques: MRE11 (6), NBS1 (6), APLF (46), XRCC1 (57), CHD4 (58), NuRD (59) and ALC1 (47).”
BackgroundDNAPKcs-dependent arrest of RNA polymerase II transcription in the presence of DNA breaks
Pankotai, T., Bonhomme, C., Chen, D., & Soutoglou, E. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2224 · Cited by 239
Cited by
The DBHS proteins SFPQ, NONO and PSPC1: a multipurpose molecular scaffold
Knott, G. J., Bond, C. S., & Fox, A. H. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw271 · Cited by 326
Role for DNA Damage Signaling in Pulmonary Arterial Hypertension
Meloche, J., Pflieger, A., Vaillancourt, M., Paulin, R., Potus, F., Zervopoulos, S., Graydon, C., Courboulin, A., Breuils-Bonnet, S., Tremblay, È., et al. (2014) · Circulation · doi:10.1161/circulationaha.113.006167 · Cited by 254
From “Cellular” RNA to “Smart” RNA: Multiple Roles of RNA in Genome Stability and Beyond
Michelini, F., Jalihal, A. P., Francia, S., Meers, C., Neeb, Z. T., Rossiello, F., Gioia, U., Aguado, J., Jones-Weinert, C., Luke, B., et al. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00487 · Cited by 85
Poly(ADP-Ribose) Polymerases in Host-Pathogen Interactions, Inflammation, and Immunity
Brady, P. N., Goel, A., & Johnson, M. A. (2019) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00038-18 · Cited by 58
Metadata
- Authors
- Krietsch, J., Caron, M.-C., Gagné, J.-P., Ethier, C., Vignard, J., Vincent, M., Rouleau, M., Hendzel, M. J., Poirier, G. G., & Masson, J.-Y.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-08-30
- Volume
- 40
- Issue
- 20
- Pages
- 10287-10301
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2048465335
- Cited by
- 163
- References
- 7
- FWCI
- 3.95
- Type
- journal article
- Language
- en
- Open access
- gold
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- Krietsch, J., Caron, M.-C., Gagné, J.-P., Ethier, C., Vignard, J., Vincent, M., Rouleau, M., Hendzel, M. J., Poirier, G. G., & Masson, J.-Y. (2012). PARP activation regulates the RNA-binding protein NONO in the DNA damage response to DNA double-strand breaks. Nucleic Acids Research. https://doi.org/10.1093/nar/gks798
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