The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Automated physics-based design of synthetic riboswitches from diverse RNA aptamers
Open accessAbstract
Riboswitches are shape-changing regulatory RNAs that bind chemicals and regulate gene expression, directly coupling sensing to cellular actuation. However, it remains unclear how their sequence controls the physics of riboswitch switching and activation, particularly when changing the ligand-binding aptamer domain. We report the development of a statistical thermodynamic model that predicts the sequence-structure-function relationship for translation-regulating riboswitches that activate gene expression, characterized inside cells and within cell-free transcription-translation assays. Using the model, we carried out automated computational design of 62 synthetic riboswitches that used six different RNA aptamers to sense diverse chemicals (theophylline, tetramethylrosamine, fluoride, dopamine, thyroxine, 2,4-dinitrotoluene) and activated gene expression by up to 383-fold. The model explains how aptamer structure, ligand affinity, switching free energy and macromolecular crowding collectively control riboswitch activation. Our model-based approach for engineering riboswitches quantitatively confirms several physical mechanisms governing ligand-induced RNA shape-change and enables the development of cell-free and bacterial sensors for diverse applications.
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Macromolecular crowding: obvious but underappreciated
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Automated design of synthetic ribosome binding sites to control protein expression
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Self-citationHigh-Resolution Molecular Discrimination by RNA
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Gene regulation by riboswitches
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Toehold Switches: De-Novo-Designed Regulators of Gene Expression
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Self-citationWidespread genetic switches and toxicity resistance proteins for fluoride
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Cell-free gene expression: an expanded repertoire of applications
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Metadata
- Authors
- Espah Borujeni, A., Mishler, D. M., Wang, J., Huso, W., & Salis, H. M.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-11-30
- Volume
- 44
- Issue
- 1
- Pages
- 1-13
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2230709708
- Cited by
- 264
- References
- 42
- FWCI
- 11.06
- Type
- journal article
- Language
- en
- Open access
- gold
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