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Analysis of translation using polysome profiling
Open accessAbstract
During the past decade, there has been growing interest in the role of translational regulation of gene expression in many organisms. Polysome profiling has been developed to infer the translational status of a specific mRNA species or to analyze the translatome, i.e. the subset of mRNAs actively translated in a cell. Polysome profiling is especially suitable for emergent model organisms for which genomic data are limited. In this paper, we describe an optimized protocol for the purification of sea urchin polysomes and highlight the critical steps involved in polysome purification. We applied this protocol to obtain experimental results on translational regulation of mRNAs following fertilization. Our protocol should prove useful for integrating the study of the role of translational regulation in gene regulatory networks in any biological model. In addition, we demonstrate how to carry out high-throughput processing of polysome gradient fractions, for the simultaneous screening of multiple biological conditions and large-scale preparation of samples for next-generation sequencing.
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Global quantification of mammalian gene expression control
Schwanhäusser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., & Selbach, M. (2011) · Nature · doi:10.1038/nature10098 · Cited by 6,566 · cited 2×
“Accordingly, almost half of the variation of protein concentration is due to translational control (3).”
BackgroundMethodGlobal signatures of protein and mRNA expression levels
de Sousa Abreu, R., Penalva, L. O., Marcotte, E. M., & Vogel, C. (2009) · Molecular BioSystems · doi:10.1039/b908315d · Cited by 1,248
Cell type–specific mRNA purification by translating ribosome affinity purification (TRAP)
Heiman, M., Kulicke, R., Fenster, R. J., Greengard, P., & Heintz, N. (2014) · Nature Protocols · doi:10.1038/nprot.2014.085 · Cited by 498 · cited 1×
“Tagged ribosomes are affinity-purified and associated mRNAs are identified by microarray or deep-sequencing (10).”
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Research works printed but not linked: 39
- 10.1038/nrm2838 · DOI · Paper page
- 10.1101/cshperspect.a011536 · DOI · Paper page
- 10.1093/nar/gkt178 · DOI · Paper page
- 10.1038/nrg3185 · DOI · Paper page
- 10.1016/j.cell.2014.02.033 · DOI · Paper page
- 10.1093/bfgp/elu045 · DOI
- 10.1016/j.cell.2016.02.066 · DOI · Paper page
- 10.1091/mbc.E11-09-0768 · DOI
- 10.1261/rna.79407 · DOI
- 10.1371/journal.pone.0150318 · DOI
- 10.1101/gad.2028911 · DOI · Paper page
- Larsson O. Tian B. Sonenberg N. Toward a genome-wide landscape of translational control. Cold Spring Harb. Perspect. Biol. 2012:209-223. · DOI
- Kuersten, 2013, Translation regulation gets its ‘omics’ moment, Wiley Interdiscip. Rev. RNA, 4, 617, 10.1002/wrna.1173 · DOI
- 10.1016/j.celrep.2014.05.002 · DOI
- 10.1101/cshperspect.a011536 · DOI · Paper page
- Davidson E.H. Gene Activity in Early Development. 3rd Edn. NY: Academic Press; 1986.
- 10.1002/dvdy.21454 · DOI · Paper page
- 10.1016/j.ydbio.2006.07.036 · DOI
- Cormier P. Chassé H. Cosson B. Mulner-Lorillon O. Morales J. Translational control in echinoderms: the calm before the storm. In: Hernández G Jagus R , editors. Evolution of the Protein Synthesis Machinery and its Regulation. Switzerland: Springer; 2016. p. 413-434. · doi:10.1007/978-3-319-39468-8_16
- 10.1016/0012-1606(85)90470-1 · doi:10.1016/0012-1606(85)90470-1
- 10.1002/dvg.1020140510 · doi:10.1002/dvg.1020140510
- 10.1038/sj.embor.7401043 · DOI · Paper page
- 10.1016/0022-2836(67)90352-X · doi:10.1016/0022-2836(67)90352-x
- 10.1016/S0091-679X(04)74023-6 · doi:10.1016/s0091-679x(04)74023-6
- 10.1083/jcb.49.3.692 · doi:10.1083/jcb.49.3.692
- 10.1371/journal.pone.0000242 · DOI · Paper page
- 10.1016/0040-8166(94)90013-2 · doi:10.1016/0040-8166(94)90013-2
- 10.1093/nar/19.21.6051 · DOI
- Morrison, 1977, Quantitation and characterisation of poly(A)-containing messenger RNAs from mouse neuroblastoma cells, BBA Sect. Nucleic Acids Protein Synth., 476, 228
- 10.1073/pnas.1307747110 · DOI · Paper page
- 10.1073/pnas.70.12.3866 · DOI
- 10.1073/pnas.68.2.390 · DOI · Paper page
- 10.1152/ajpcell.00166.2010 · DOI
- 10.1016/0092-8674(83)90420-8 · DOI · Paper page
- Kelso-Winemiller, 1991, ‘Unmasking’ of stored maternal mRNAs and the activation of protein synthesis at fertilization in sea urchins, Development, 111, 623, 10.1242/dev.111.2.623 · doi:10.1242/dev.111.2.623
- 10.1083/jcb.100.6.1968 · DOI
- 10.1093/nar/gkn887 · DOI · Paper page
- 10.1016/j.crvi.2011.02.009 · DOI · Paper page
- 10.1091/mbc.E15-10-0682 · DOI
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Suppression of Exosomal PD-L1 Induces Systemic Anti-tumor Immunity and Memory
Poggio, M., Hu, T., Pai, C.-C., Chu, B., Belair, C. D., Chang, A., Montabana, E., Lang, U. E., Fu, Q., Fong, L., et al. (2019) · Cell · doi:10.1016/j.cell.2019.02.016 · Cited by 1,376
Detection of immunogenic cell death and its relevance for cancer therapy
Fucikova, J., Kepp, O., Kasikova, L., Petroni, G., Yamazaki, T., Liu, P., Zhao, L., Spisek, R., Kroemer, G., & Galluzzi, L. (2020) · Cell Death & Disease · doi:10.1038/s41419-020-03221-2 · Cited by 1,138
Changes in m6A RNA Methylation Contribute to Heart Failure Progression by Modulating Translation
Berulava, T., Buchholz, E., Elerdashvili, V., Pena, T., Islam, M. R., Lbik, D., Mohamed, B. A., Renner, A., von Lewinski, D., Sacherer, M., et al. (2020) · European Journal of Heart Failure · doi:10.1002/ejhf.1672 · Cited by 323
Jian, D., Wang, Y., Jian, L., Tang, H., Rao, L., Chen, K., Jia, Z., Zhang, W., Liu, Y., Chen, X., et al. (2020) · Theranostics · doi:10.7150/thno.45178 · Cited by 281
Variant ribosomal RNA alleles are conserved and exhibit tissue-specific expression
Parks, M. M., Kurylo, C. M., Dass, R. A., Bojmar, L., Lyden, D., Vincent, C. T., & Blanchard, S. C. (2018) · Science Advances · doi:10.1126/sciadv.aao0665 · Cited by 260
Metadata
- Authors
- Chassé, H., Boulben, S., Costache, V., Cormier, P., & Morales, J.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-10-07
- Volume
- 45
- Issue
- 3
- Pages
- gkw907
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2529733312
- Cited by
- 202
- References
- 3
- FWCI
- 5.72
- Type
- journal article
- Language
- en
- Open access
- gold
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