Light-Induced Artemisinin Biosynthesis Is Regulated by the bZIP Transcription Factor AaHY5 in Artemisia annua
Abstract
Artemisinin, the frontline drug against malaria, is a sesquiterpenoid extracted from Artemisia annua. Light has been proposed to play an important role in the activation of artemisinin biosynthesis. Here, we report the basic leucine zipper transcription factor (TF) AaHY5 as a key regulator of light-induced biosynthesis of artemisinin. We show that AaHY5 transcription overlaps with that of artemisinin biosynthesis genes in response to light and in A. annua tissues. Analysis of AaHY5 overexpression and RNAi-suppression lines suggests that AaHY5 is a positive regulator of the expression of artemisinin biosynthesis genes and accumulation of artemisinin. We show that AaHY5 complements the hy5 mutant in Arabidopsis thaliana. Our data further suggest that AaHY5 interacts with AaCOP1, the ubiquitin E3 ligase CONSTITUTIVE PHOTOMORPHOGENIC1 in A. annua. In yeast one-hybrid and transient expression assays, we demonstrate that AaHY5 acts via the TF GLANDULAR TRICHOME-SPECIFIC WRKY 1 (AaGSW1) in artemisinin regulation. In summary, we present a novel regulator of artemisinin gene expression and propose a model in which AaHY5 indirectly controls artemisinin production in response to changing light conditions.
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Various environmental stresses like heavy metal pollution, cold, water (drought and flooding), light, pest and disease damage affect accumulation of specialty metabolites in plants including artemisinin in A. annua.
“Besides elicitors and plant growth regulators, various environmental stresses like heavy metal pollution, cold, water (drought and flooding), light, pest and disease damage affect accumulation of specialty metabolites in plants including artemisinin in A. annua (Xiang et al. 2019; Hao et al. 2019; Soni and Abdin 2017; Yin et al. 2008).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Hao et al. 2019
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- Authors
- Hao, X., Zhong, Y., N�tzmann, H.-W., Fu, X., Yan, T., Shen, Q., Chen, M., Ma, Y., Zhao, J., Osbourn, A., Li, L., & Tang, K.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Department of Biology and Biochemistry, The Milner Centre for Evolution, University of Bath, Claverton Down, Bath, UK
- John Innes Centre (GB)
- Corresponding author
- Li, Ling
- Tang, Kexuan
- ORCID
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2019-05-10
- Volume
- 60
- Issue
- 8
- Pages
- 1747-1760
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2944489685
- Cited by
- 121
- References
- 54
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 26 · 2023: 21 · 2022: 21 · 2021: 15 · 2020: 13 · 2019: 1
- FWCI
- 4.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Plant and Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31770327)
- China National Transgenic Plant Research and Commercialization Project (2016ZX08002-001)
- Shanghai Natural Science Foundation (16ZR1418000)
- China Scholarship Council
- CSC
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Keywords
- artemisinin biosynthesis, Artemisia annua, bZIP transcription factors, light regulation, E3 ubiquitin ligase, WRKY transcription factors, sesquiterpene biosynthesis, gene expression regulation
- MeSH terms
- Light, Plant Proteins, Transcription Factors, Gene Expression Regulation, Plant, Plants, Genetically Modified, Artemisia annua, Artemisinins
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