The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Silver Nanoparticle Induced Blood-Brain Barrier Inflammation and Increased Permeability in Primary Rat Brain Microvessel Endothelial Cells
Abstract
The current report examines the interactions of silver nanoparticles (Ag-NPs) with the cerebral microvasculature to identify the involvement of proinflammatory mediators that can increase blood-brain barrier (BBB) permeability. Primary rat brain microvessel endothelial cells (rBMEC) were isolated from adult Sprague-Dawley rats for an in vitro BBB model. The Ag-NPs were characterized by transmission electron microscopy (TEM), dynamic light scattering, and laser Doppler velocimetry. The cellular accumulation, cytotoxicity (6.25-50 μg/cm(3)) and potential proinflammatory mediators (interleukin [IL]-1β, IL-2, tumor necrosis factor [TNF] α, and prostaglandin E(2) [PGE(2)]) of Ag-NPs (25, 40, or 80 nm) were determined spectrophotometrically, cell proliferation assay (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) and ELISA. The results show Ag-NPs-induced cytotoxic responses at lower concentrations for 25 and 40 nm when compared with 80-nm Ag-NPs. The proinflammatory responses in this study demonstrate both Ag-NPs size and time-dependent profiles, with IL-1B preceding both TNF and PGE(2) for 25 nm. However, larger Ag-NPs (40 and 80 nm) induced significant TNF responses at 4 and 8 h, with no observable PGE(2) response. The increased fluorescein transport observed in this study clearly indicates size-dependent increases in BBB permeability correlated with the severity of immunotoxicity. Together, these data clearly demonstrate that larger Ag-NPs (80 nm) had significantly less effect on rBMEC, whereas the smaller particles induced significant effects on all the end points at lower concentrations and/or shorter times. Further, this study suggests that Ag-NPs may interact with the cerebral microvasculature producing a proinflammatory cascade, if left unchecked; these events may further induce brain inflammation and neurotoxicity.
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Self-citationInflammatory Mediators and Stroke: New Opportunities for Novel Therapeutics
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The influence of cytokines on the integrity of the blood-brain barrier in vitro
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Antiangiogenic properties of silver nanoparticles
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Self-citationDistribution, Translocation and Accumulation of Silver Nanoparticles in Rats
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Self-citationBlood-Brain Barrier Tight Junction Disruption in Human Immunodeficiency Virus-1 Encephalitis
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Metadata
- Authors
- Trickler, W. J., Lantz, S. M., Murdock, R. C., Schrand, A. M., Robinson, B. L., Newport, G. D., Schlager, J. J., Oldenburg, S. J., Paule, M. G., Slikker, W., Hussain, S. M., & Ali, S. F.
- Venue
- Toxicological Sciences
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-08-16
- Volume
- 118
- Issue
- 1
- Pages
- 160-170
- ISSN
- 1096-0929 (ISSN-L)
- 1096-6080
- OpenAlex
- W2124693318
- Cited by
- 346
- References
- 58
- FWCI
- 9.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Trickler, W. J., Lantz, S. M., Murdock, R. C., Schrand, A. M., Robinson, B. L., Newport, G. D., Schlager, J. J., Oldenburg, S. J., Paule, M. G., Slikker, W., Hussain, S. M., & Ali, S. F. (2010). Silver Nanoparticle Induced Blood-Brain Barrier Inflammation and Increased Permeability in Primary Rat Brain Microvessel Endothelial Cells. Toxicological Sciences. https://doi.org/10.1093/toxsci/kfq244
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