Cited by 204Plant-Microbe Interactions and ImmunityPolysaccharides and Plant Cell WallsImmune Response and Inflammation

Sulfated Fucan Oligosaccharides Elicit Defense Responses in Tobacco and Local and Systemic Resistance Against Tobacco Mosaic Virus

Klarzynski, Olivier, Descamps, Valérie, Plesse, Bertrand, Yvin, Jean-Claude, Kloareg, Bernard, Fritig, Bernard·2003·Molecular Plant-Microbe Interactions

Abstract

Sulfated fucans are common structural components of the cell walls of marine brown algae. Using a fucan-degrading hydrolase isolated from a marine bacterium, we prepared sulfated fucan oligosaccharides made of mono- and disulfated fucose units alternatively bound by alpha-1,4 and alpha-1,3 glycosidic linkages, respectively. Here, we report on the elicitor activity of such fucan oligosaccharide preparations in tobacco. In suspension cell cultures, oligofucans at the dose of 200 microg ml(-1) rapidly induced a marked alkalinization of the extracellular medium and the release of hydrogen peroxide. This was followed within a few hours by a strong stimulation of phenylalanine ammonia-lyase and lipoxygenase activities. Tobacco leaves treated with oligofucans locally accumulated salicylic acid (SA) and the phytoalexin scopoletin and expressed several pathogenesis-related (PR) proteins, but they displayed no symptoms of cell death. Fucan oligosaccharides also induced the systemic accumulation of SA and the acidic PR protein PR-1, two markers of systemic acquired resistance (SAR). Consistently, fucan oligosaccharides strongly stimulated both local and systemic resistance to tobacco mosaic virus (TMV). The use of transgenic plants unable to accumulate SA indicated that, as in the SAR primed by TMV, SA is required for the establishment of oligofucan-induced resistance.

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Crossref · 10.1094/mpmi.2003.16.2.115

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Authors
Klarzynski, O., Descamps, V., Plesse, B., Yvin, J.-C., Kloareg, B., & Fritig, B.
Affiliations
Centre National de la Recherche Scientifique (FR)
Venue
Molecular Plant-Microbe Interactions
Published
2003-02
Volume
16
Issue
2
Pages
115-122
ISSN
  • 0894-0282 (ISSN-L)
  • 1943-7706
OpenAlex
W2009051575
Cited by
204
References
51
Citations by year
2026: 6 · 2025: 7 · 2024: 8 · 2023: 9 · 2022: 20 · 2021: 12 · 2020: 15 · 2019: 11 · 2018: 10 · 2017: 5 · 2016: 11 · 2015: 7 · 2014: 14 · 2013: 9 · 2012: 10
FWCI
7.10
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Plant-Microbe Interactions (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
elicitors, plant defense response, systemic acquired resistance, tobacco mosaic virus, salicylic acid, scopoletin, pathogenesis-related proteins, phenylalanine ammonia-lyase, lipoxygenase, hydrogen peroxide, transgenic plants, plant virus resistance
MeSH terms
Phaeophyceae, Cells, Cultured, Hydrogen Peroxide, Immunity, Innate, Lipoxygenase, Oligosaccharides, Phenylalanine Ammonia-Lyase, Plant Diseases, Plant Proteins, Polysaccharides, Scopoletin, Nicotiana, Tobacco Mosaic Virus, Signal Transduction, Salicylic Acid, Plants, Genetically Modified

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