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Cited by 232

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

Nitric oxide triggers enhanced induction of vascular endothelial growth factor expression in cultured keratinocytes (HaCaT) and during cutaneous wound repair

Frank, Stefan, Stallmeyer, Birgit, Kämpfer, Heiko, Kolb, Nicole, Pfeilschifter, Josef·1999·The FASEB Journal
DOI 10.1096/fasebj.13.14.2002
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Abstract

Recently, we demonstrated a large induction of inducible nitric oxide synthase (iNOS) during cutaneous wound repair. In this study, we investigated the role of nitric oxide (NO) for the expression of vascular endothelial growth factor (VEGF), which represents the most important angiogenic factor during the proliferative phase of skin repair. Since keratinocytes are the major source of VEGF production during this process, we used cultured keratinocytes (HaCaT cell line) as an in vitro model to investigate NO action on growth factor‐ and cytokine‐stimulated VEGF expression. Exogenously added NO enhanced transforming growth factor‐β1‐, keratinocyte growth factor‐, interleukin‐1β‐, tumor necrosis factor‐α‐, and interferon‐γ‐induced VEGF mRNA and protein synthesis in keratinocytes. We could demonstrate that high‐level expression of cytokine‐induced VEGF mRNA in keratinocytes is dependent on endogenously produced NO, as inhibition of the coinduced iNOS by N G ‐monomethyl‐L‐arginine (L‐NMMA) markedly decreased cytokine‐triggered VEGF mRNA levels in the cells. We also established an in vivo model in mice to investigate the role of NO during wound healing. During excisional wound repair, mice were treated with L‐N 6 ‐(1‐iminoethyl)lysine (L‐NIL), a selective inhibitor of iNOS enzymatic activity. Compared to control mice, L‐NIL‐treated animals were characterized by markedly reduced VEGF mRNA levels during the inflammatory phase of repair. Immunohistochemistry demonstrated reduced VEGF protein expression and a completely disorganized pattern of VEGF‐expressing keratinocytes within the hyperproliferative epithelium at the wound edge in L‐NIL‐treated mice. We demonstrate that triggering of VEGF expression is a crucial molecular mechanism underlying NO function during wound healing.— Frank, S., Stallmeyer, B., Kämpfer, H., Kolb, N., Pfeilschifter, J. Nitric oxide triggers enhanced induction of vascular endothelial growth factor expression in cultured keratinocytes (HaCaT) and during cutaneous wound repair. FASEB J . 13, 2002–2014 (1999)

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Show all 5 references

Research works printed but not linked: 52

  1. Clark R. A. F., 1996, The Molecular and Cellular Biology of Wound Repair, 3
  2. 10.1096/fasebj.6.12.1381691 · DOI · Paper page
  3. 10.1146/annurev.bi.63.070194.001135 · DOI · Paper page
  4. 10.1016/S0021-9258(17)36703-0 · DOI · Paper page
  5. 10.1515/bchm3.1995.376.3.179 · DOI · Paper page
  6. 10.1016/S0021-9258(18)82401-2 · DOI · Paper page
  7. 10.1111/1523-1747.ep12328204 · DOI
  8. 10.1111/1523-1747.ep12363167 · DOI · Paper page
  9. 10.1172/JCI119206 · DOI · Paper page
  10. 10.1172/JCI2067 · DOI · Paper page
  11. 10.1046/j.1523-1747.1998.00434.x · DOI
  12. 10.1242/jeb.202.6.645 · DOI · Paper page
  13. 10.1038/sj.onc.1201201 · DOI · Paper page
  14. 10.1038/nm0897-879 · DOI · Paper page
  15. 10.1002/jlb.59.4.471 · DOI
  16. Frank S., 1999, Differential regulation of vascular endothelial growth factor and its receptor fms‐like‐tyrosine kinase is mediated by nitric oxide in rat renal mesangial cells, Biochem. J., 338, 367, 10.1042/bj3380367 · DOI
  17. 10.1210/mend-5-12-1806 · DOI · Paper page
  18. 10.1126/science.2479986 · DOI · Paper page
  19. 10.1126/science.2479987 · DOI · Paper page
  20. 10.1073/pnas.86.19.7311 · DOI · Paper page
  21. 10.1172/JCI114322 · DOI · Paper page
  22. Brown L. F., 1988, Fibrinogen influx and accumulation of cross‐linked fibrin in healing wounds and in tumor stroma, Am. J. Pathol., 130, 455
  23. Nissen N. N., 1998, Vascular endothelial growth factor mediates angiogenic activity during the proliferative phase of wound healing, Am. J. Pathol., 152, 1445
  24. 10.1074/jbc.270.21.12607 · DOI · Paper page
  25. 10.2337/diab.31.1.S1 · DOI · Paper page
  26. 10.1021/jm00049a007 · DOI · Paper page
  27. 10.1016/0014-2999(95)00618-4 · DOI
  28. 10.1016/S1074-7613(00)80460-4 · DOI · Paper page
  29. 10.1016/0003-2697(87)90021-2 · DOI · Paper page
  30. 10.1073/pnas.89.15.6896 · DOI · Paper page
  31. 10.1242/dev.114.2.521 · DOI · Paper page
  32. 10.1016/S0006-291X(05)80313-4 · DOI · Paper page
  33. 10.1016/S0040-4039(00)98368-0 · DOI · Paper page
  34. 10.1046/j.1523-1747.1998.00433.x · DOI
  35. 10.3109/08977199309046931 · DOI
  36. 10.1073/pnas.86.3.802 · DOI · Paper page
  37. 10.1126/science.7973639 · DOI · Paper page
  38. 10.1016/S0002-9440(10)65362-X · DOI
  39. 10.1126/science.276.5309.75 · DOI · Paper page
  40. 10.1111/j.1346-8138.1997.tb02748.x · DOI
  41. 10.1046/j.1365-2133.1997.d01-1136.x · DOI
  42. 10.1084/jem.184.5.2007 · DOI · Paper page
  43. 10.1111/j.1365-2133.1996.tb06963.x · DOI
  44. 10.1007/s004030050268 · DOI
  45. Barbul A. S., 1990, Arginine enhances wound healing and lymphocyte immune responses in humans, Surgery, 108, 331
  46. Kirk S. J., 1993, Arginine stimulates wound healing and immune function in elderly human beings, Surgery, 114, 155
  47. Seifter E., 1978, Arginine: an essential amino acid for injured rats, Surgery, 84, 224
  48. Albina J. E., 1990, Temporal expression of different pathways of 1‐arginine metabolism in healing wounds, J. Immunol., 144, 3877, 10.4049/jimmunol.144.10.3877 · DOI · Paper page
  49. 10.1126/science.1312256 · DOI · Paper page
  50. 10.1111/1523-1747.ep12312542 · DOI · Paper page
  51. Wong H. L., 1989, Human Monocytes, 383
  52. 10.1016/S0002-9440(10)65601-5 · DOI · Paper page

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Metadata

Authors
Frank, S., Stallmeyer, B., Kämpfer, H., Kolb, N., & Pfeilschifter, J.
Venue
The FASEB Journal
Publisher
Wiley
Published
1999-11
Volume
13
Issue
14
Pages
2002-2014
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
DOI
10.1096/fasebj.13.14.2002
OpenAlex
W1934754959
Cited by
232
References
5
FWCI
12.40
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Funders
Deutsche Forschungsgemeinschaft (SFB 553)

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CoChat. (2026). Citation check: Nitric oxide triggers enhanced induction of vascular endothelial growth factor expression in cultured keratinocytes (HaCaT) and during cutaneous wound repair. https://app.cochat.ai/papers/10.1096/fasebj.13.14.2002
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Frank, S., Stallmeyer, B., Kämpfer, H., Kolb, N., & Pfeilschifter, J. (1999). Nitric oxide triggers enhanced induction of vascular endothelial growth factor expression in cultured keratinocytes (HaCaT) and during cutaneous wound repair. The FASEB Journal. https://doi.org/10.1096/fasebj.13.14.2002

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.